Acetamide, N,N'-(ethenylmethylsilylene)bis[N-ethyl-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

30 July - 26 October, 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2010

Report date:

2010

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-[ethenyl(N-ethylacetamido)methylsilyl]-N-ethylacetamide

EC Number:

643-078-7

Cas Number:

87855-59-2

Molecular formula:

C11 H22 N2 O2 Si Si(CH3)(CH=CH2)[N(CH2CH3)(C(=O)CH3)]2

IUPAC Name:

N-[ethenyl(N-ethylacetamido)methylsilyl]-N-ethylacetamide

Test material form:

solid: compact

Details on test material:

Identification: Methylvinyl bis(n-ethylacetamido)silane (supplied as Dow Corning® 1-6008)
Lot No.: 091217
CAS No.: 87855-59-2
Expiration Date: 26 December 2010
Source: Korea Biogen Co. Ltd., 690 Sinduk-Ri, Sungnam-Myuan, Chunan-City, Chungnam, 330-893, Korea
Physical Description: Amber Liquid (StultS, 2010)
Stability; Stable, refer to MSDS
Purity: 77.9 ± 0.3% area % purity (Stuns, 2010), Tested as 100%
Solubility: Unknown, andlor may be soluble in other common organic solvents
Storage Conditions: Room Temperature













Room Temperature

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Results and discussion

Any other information on results incl. tables

The 48-hour EC50 value for methylvinyl his(n-ethy\acetamido )silane and Daplmia magna was determined using probit analysis to be 69 rng/L, with 95% confidence intervals of 57 to 88 mgIL. The highest concentration producing 0% immobilization was 25 rng/L and the lowest concentration producing 100% immobilization was> 100 mgIL. The No-Observed-Effect Concentration (NOEC) was determined to be 25 mgIL.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The No-Observed-Effect Concentration (NOEC) was determined to be 25 mg/L.

Executive summary:

The purpose of this study was to estimate the acute toxicity of the hydrolysis products of methyl vinyl bis(n-ethylacetamido )silane to daphnids (Daphnia magna) under static conditions. Based on preliminary range-finding test results and consultation with the Study Sponsor, the daphnids were exposed to nominal concentrations of 6.3, 13,25, 50 and 100 mg/L. Due to the rapid hydrolysis of the test substance (information based on previous work and consultation with the study monitor), no attempt was made to determine the toxicity of parent substance. The results of this study are based on nominal concentrations. Twenty daphnids were exposed for 48 hours in four replicate test vessels for each concentration and the control. The immobilization in each test vessel was monitored and recorded at test initiation and after 24 and 48 hours of exposure. Temperature, pH and dissolved oxygen were measured daily during the test and were within acceptable limits. Following 48 hours of exposure, 30 and 75% immobilization was observed among daphnids exposed to the 50 and 100 mg/L treatment levels, respectively. One surviving daphnid exposed to the 100 mgIL treatment level was observed to be lethargic at test termination. No immobilization or adverse effects were observed in the remaining treatment levels tested (6.3, 13 and 25 mglL) or the control. The 48-hour EC50 value for methylvinyl bis(n-ethylacetamido )silane and Daphnia magna was determined using probit analysis to be 69 mg/L, with 95% confidence intervals of 57 to 88 mglL. The highest concentration producing 0% immobilization was 25 mg/L and the lowest concentration producing 100% immobilization was> 100 mg/L. The No-Observed-Effect Concentration (NOEC) was determined to be 25 mg/L.