Phosphonic acid, P-phenyl-, zinc salt (1:1)

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

20 to 22 January 2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study undertaken to GLP using internationally accepted guidelines.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

other: stated in Method oj Testing New Chemical Substances (November 21,2003, Yakushoku hatsu No. 1121002, Nov. 13, 2003, Seikyoku No.2, Kanpoki hatsu No. 031121002)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

zinc(2+) phenylphosphonate

EC Number:

696-577-7

Cas Number:

34335-10-9

Molecular formula:

C6 H7 O3 P. Zn C6H5-P(=O)O2,2+Zn2-

IUPAC Name:

zinc(2+) phenylphosphonate

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): Phenylphosphonic acid zinc salt
- Substance type: Inorganic
- Physical state:Powder
- Analytical purity: > 99%
- Impurities (identity and concentrations):
- Purity test date:
- Lot/batch No.: 6381
- Expiration date of the lot/batch:
- Stability under test conditions: Stable under nonnal handling
- Storage condition of test material: Stored in desiccators in a dark place at room temperature
- Other:

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 100, 56, 31,17, and 9.5 mg/l
- Sampling method:
- Sample storage conditions before analysis:

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test sample and test water were mixed to make a 100 mg/L solution (nominal value), stirred for about 48 hours, and filtrated. The solution was diluted with the test water to prepare stock solution.
- Eluate: Dechlorinated tap water
- Differential loading:
- Controls: One

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Water flea (Daphnia magna)
- Strain: Clone A
- Source: The test organisms were descendants of Daphnia magna (Clone A) purchased from the University of Sheffield in the United Kingdom
- Method of breeding: parthenogenesis
- Feeding during test: no
- Food type: the test organisms were fed 0.1 - 0.2 mgC (amount of organic carbon content) of Chiarella vulgaris per flea per day.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Post exposure observation period:

Immobilization and conditions were observed at 24 and 48 hours after the start of exposure.

Test conditions

Hardness:

28 mg/l (CaCO3)

Test temperature:

20.1°C

pH:

7.6 (22°C)

Dissolved oxygen:

8.8 mg/l

Salinity:

9.8 mg/l (Na)

Nominal and measured concentrations:

nominal; content ratio ofthe stock solution: 100,56,31,17, and 9.5%
measured; 0.95, 1.6, 3.0, 5.4 and 9.5 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 100 ml glass beaker
- Type: closed
- Fill volume: 100 ml
- Aeration: no
- Type of flow-through (e.g. peristaltic or proportional diluter): n/a
- Renewal rate of test solution (frequency/flow rate): none
- No. of organisms per vessel: 4
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 1

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Dechlorinated tap water
- Total organic carbon: 0.9 mg/l
- Pesticides: < 0.0001 mg/l
- Chlorine: < 0.02 mg/l
- Alkalinity: 20 mg/l
- Ca/mg ratio: 8.2/1.9 mg/l
- Conductivity: 11.6 mSM
- Culture medium different from test medium: no
- Intervals of water quality measurement: 24h

OTHER TEST CONDITIONS
- Adjustment of pH: pH of the test solution was not adjusted.
- Photoperiod: Photocycle of l6-hour light and 8-hour darkness using room lights.
- Light intensity: no data

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) :

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

5.8 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% confidence limits: 4.7 -7.5 mg/L

Effect concentrations 2

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

3.3 mg/L

Nominal / measured:

meas. (initial)

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% confidence limits: 2.8 - 3.8 mgIL

Details on results:

24-hour EC,a of the test substance to the water fleas was 5.8 mg/L (95% confidence limits:
4.7 -7.5 mg/L), 48-hour EC,a was 3.3 mg/L (95% confidence limits: 2.8 - 3.8 mgIL).

Immobilization rate
The lowest concentration of 100% immobilization at 48 hour was 9.5 mgIL, and the highest concentration of0% immobilization was 1.6 mgIL. No water flea exhibited abnormal symptoms or behavior (such as discoloration or floating on the surface of the water) in the control group during exposure
period; therefore the immobilization ratio was 0%, and the validity criteria (less than 10%) ofthis test were also fulfilled.

symptom observation
No symptom was observed in the control group. The following observations are all based on comparison with the control group. Symptoms observed
during exposure period were lethargy, immobilization, and reduced activity.

Any other information on results incl. tables

Immoblization rate

Measured concentration * (mg/L)		Immobilisation rate (%)
		24 hours		48 Hours
		Individual test vessel	Individual test group	Individual test vessel	Individual test group
Control group	A	0	0	0	0
	B	0		0
	C	0		0
	D	0		0
0.95	A	0	0	0	0
	B	0		0
	C	0		0
	D	0		0
1.6	A	0	0	0	0
	B	0		0
	C	0		0
	D	0		0
3.0	A	60	20	80	40
	B	0		20
	C	0		40
	D	20		20
5.4	A	40	40	80	95
	B	20		100
	C	60		100
	D	40		100
9.5	A	100	80	100	100
	B	60		100
	C	80		100
	D	80		100

*: Geometric average of measured concentrations (hereinafter described as "measured concentration")

Observed symptoms

Content ratio of stock solution (%)	24 hour		48 hour
	Immobilisation rate (%)	Other symptoms	Immobilisation rate (%)	Other symptoms
Control group	-	-	-	-
0.95	-	-	0	-
1.6	-	-	0	RA (1)
3.0	IM (4)	RA (2)	IM (8) LETH (8)	RA (4)
5.4	IM (8) LETH (8)	-	IM (19) LETH (16)	RA (1)
9.5	IM (16) LETH (15)	-	IM (20) LETH (20)	-

"-" indicates that there was no symptom.

Abbreviations of symptoms

IM (Immobilization)

LETI-! (Lethargic)

RA (Reduced activity)

EC₅₀on the water fleas

Duration of exposure	EC50(mg/L)	95% confidence limits (mg/L) (Angle of regression line)	EC50calculation method
24 hours	5.8	4.7 – 7.5 (3.8)	Probit method
48 hours	3.3	2.8 – 3.8 (7.9)	Probit method

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

24-hour EC50, was 5.8 mg/L (95% confidence limits: 4.7 -7.5 mg/L),
48-hour EC50 was 3.3 mg/L (95% confidence limits: 2.8 - 3.8 mgIL).