Registration Dossier

Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
6 Jun 1975 - 24 Mar 1976
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Basic information is available

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Circular on Test Methods of New Chemical Substances (Japan), Biodegradation test
Deviations:
not specified
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Chloroacetic acid
EC Number:
201-178-4
EC Name:
Chloroacetic acid
Cas Number:
79-11-8
Molecular formula:
C2H3ClO2
IUPAC Name:
chloroacetic acid
Test material form:
not specified
Details on test material:
- Name of test material (as cited in study report): Chloroacetic acid
- Molecular formula (if other than submission substance): C2H2O2Cl
- Analytical purity: No data

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
not specified
Details on inoculum:
m
Duration of test (contact time):
3 wk
Parameter followed for biodegradation estimationopen allclose all
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
TOC removal
Parameter followed for biodegradation estimation:
test mat. analysis
Details on study design:
TEST CONDITIONS
- pH adjusted: yes

TEST SYSTEM
- Culturing apparatus: closed aotumatic oxgen consmuption aparatus
- Number of culture flasks/concentration: 2 abiotic control (water + test substance), 2 test suspenion (sludge + test substance), 1 inoculum blank (sludge) adn 2 medium blank (water)
- Measuring equipment: O2 control, TOC and GC

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
Reference substance
Reference substance:
not specified

Results and discussion

% Degradationopen allclose all
Parameter:
% degradation (O2 consumption)
Value:
65
Sampling time:
3 wk
Parameter:
% degradation (TOC removal)
Value:
98.8
Sampling time:
3 wk
Parameter:
% degradation (test mat. analysis)
Value:
100
Sampling time:
3 wk
Remarks on result:
other: GC
Details on results:
According to the result of coulometer after 14 days, the oxygen absorbtion curve was changed. Therefore, the test was prolonged to 3 weeks. The result was calculated based on the value of sample 3 which showed trend of degradability.

Any other information on results incl. tables

Table 1. Test result of TOC

Sample

Height of peak (mm)

Apparent Concentrationµg/ml

Residual quantity (300 ml)

test sub.

correction value by sludge

correction value by water

1. Sludge blank

5.4

 

 

 

 

3. Sludge + test sub.

5.6

0.2

 

1.0

0.3*

4. Sludge + test sub.

21.7

16.3

41.3

40.5

12.2

5. water + test sub.

44.2

 

34.3

101.0

30.3**

6. water + test sub.

37.2

 

 

85.0

25.5

water blank

2.9

 

 

 

 

* Result of this study was caluculated based on Sample 3.

** 5 was excluded because contamination was assumed.

Table 2. Result of GC determination

Sample

Mean of peak (µV sec)

Concentrationµg/ml

Residual quantity (30 ml)

Residue

Mean

(mg)

Degradation

(%)

1. Sludge blank

0

0

0

 

 

3. Sludge + test sub.

0

0

0*

-

-

4. Sludge + test sub.

279977

820

24.6

5. water + test sub.

268997

790

23.7

24.9

100

6. water + test sub.

295530

870

26.1

* Result of this study was calculated based on Sample 3.

Applicant's summary and conclusion

Interpretation of results:
readily biodegradable