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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1980
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
publication
Title:
Screening of tobacco smoke constituents for mutagenicity using the ames test
Author:
Florin, I., et al.
Year:
1980
Bibliographic source:
Toxicology, volume 18, 1980, page 219-232

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: refer to article
Deviations:
no
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2-phenylethanol
EC Number:
200-456-2
EC Name:
2-phenylethanol
Cas Number:
60-12-8
Molecular formula:
C8H10O
IUPAC Name:
2-phenylethanol

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with
Metabolic activation system:
Histidine and biotin
Test concentrations with justification for top dose:
0,05 micromol histidine/biotin; 10 micromol histidine and 0,05 micromol biotin
Vehicle / solvent:
Revertants were scored on glucosenminimal salts medium supplemented with 0,05 micromol histidine and 0,05 micromol biotin. Plates used for viable counts contained 10 micromol histidine (and 0,05 micromol biotin).
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
yes
Positive control substance:
other: N-methyl-N-nitro-N-nitrosoguanidin and 2-aminoanthracene

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
not applicable
Untreated negative controls validity:
not applicable
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion