L-Valine, N-[(1,1-dimethylethoxy)carbonyl]-, 2-[(2-amino-1,6-dihydro-6-oxo-9H-purin-9-yl)methoxy]ethyl ester

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2011

Report date:

2011

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: guidance document on the grouping of chemicals (Joint meeting of the chemicals committee and the working party on chemical, pesticides and biotechnology, ENV/JM/MONO(2007)28)

Principles of method if other than guideline:

The endpoint information of the Valacyclovir, selected as source chemical or analog, was used to predict the same endpoints for the target chemical, i.e. L-Valine, N-[(1,1-dimethylethoxy)carbonyl]-, 2-[(2-amino-1,6-dihydro-6-oxo-9H-purin-9-yl)methoxy]ethyl ester, which was considered to be similar to Valacyclovir on the basis of structural similarity.

GLP compliance:

no

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

124832-26-4

EC Number:

603-015-6

Cas Number:

124832-26-4

IUPAC Name:

124832-26-4

Constituent 2

Reference substance name:

Valacyclovir

IUPAC Name:

Valacyclovir

Details on test material:

- Name of test material (as cited in study report): Valacyclovir
- Molecular formula (if other than submission substance): C13H20N6O4

Results and discussion

Test results

Species / strain:

not specified

Metabolic activation:

not specified

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

not specified

Vehicle controls validity:

not specified

Untreated negative controls validity:

not specified

Positive controls validity:

not specified

Additional information on results:

No evidence of DNA damage was reported in the Ames bacterial reverse mutation assay performed on source chemical Valacyclovir.

Any other information on results incl. tables

The read across approach could be applied because it was noted that the target carbamate is more precisely a tert-butyl carbamate, known to be very stable towards most nucleophiles and bases, very little reactive and thus not likely to play an important role on the genotoxicity of the target chemical.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

It is concluded that the two structures are similar enough with respect to the physicochemical properties relevant to mutagenicity to support the read-across.
Therefore the experimental test result of the source chemical can be read-across to L-Valine, N-[(1,1-dimethylethoxy)carbonyl]-, 2-[(2-amino-1,6-dihydro-6-oxo-9H-purin-9-yl)methoxy]ethyl ester, concluding that L-Valine, N-[(1,1-dimethylethoxy)carbonyl]-, 2-[(2-amino-1,6-dihydro-6-oxo-9H-purin-9-yl)methoxy]ethyl ester is NOT MUTAGEN.