Registration Dossier

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1991-06-10
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP Guideline study, no deficiencies

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
sodium/triethanolamine 4-((2-hydroxyethyl)amino)-3-pentaproenyl-4-oxobutanoate
EC Number:
800-765-8
Cas Number:
1424149-03-0
Molecular formula:
C21H40NO4.1/2Na.1/2C6H15NO3
IUPAC Name:
sodium/triethanolamine 4-((2-hydroxyethyl)amino)-3-pentaproenyl-4-oxobutanoate
Details on test material:
Lot OP/1 4/91
ca. 80% solid test item
ca. 10% olefin
ca. 10% water
brown liquid
pH 9 at ca. 10 g/L

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge, domestic
Details on inoculum:
non adapted inoculum from municpal sewage treatment plant Hildesheim
washed 2x
dry weight 2,59 g/L

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Hardness:
not given
Test temperature:
18-22 deg c
pH:
continously mesured, guideline conform
Dissolved oxygen:
saturation monitored
Nominal and measured concentrations:
100-180-320-580-1000 mg/L
Reference substance (positive control):
yes
Remarks:
Copper (II) sulfate

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
180 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 1 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
inhibition of total respiration
Remarks:
respiration rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The registration substance was tested in an OECD 209 Respiration inhibition test and from the results a EC50(3h) of > 1000 mg/L was derived. The NOEC (3h) is 180 mg/L.