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EC number: 700-527-2 | CAS number: 1271488-66-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 10 November - 15 December 2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted by GLP accredited laboratory. Method according to OECD guideline.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 011
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.1 tris (Acute Oral Toxicity - Acute Toxic Class Method)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: JMAFF guidelines (2000) including revisions
- Deviations:
- no
- GLP compliance:
- yes
- Test type:
- acute toxic class method
- Limit test:
- yes
Test material
- Reference substance name:
- methyl trans-3-oxo-2-pentylcyclopentanecarboxylate
- EC Number:
- 700-527-2
- Cas Number:
- 1271488-66-4
- Molecular formula:
- C12H20O3
- IUPAC Name:
- methyl trans-3-oxo-2-pentylcyclopentanecarboxylate
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- Wistar rats strain Crl:WI (Han) (outbred, SPF quality) from Charles River Deutschland, Sulzfeld, Germany. Animals 9-10 weeks old were selected having body weights +/-20% of the sex mean.
A controlled environment was maintained in the room with optimal conditions of approximately 15/h air changes, a temperature of 19.5-23.6ºC, a relative humidity of 32-60% and a 12 hour artificial fluorescent light/12 hour dark cycle per day.
3 animals were present per cage in labeled Macrolon cages containing sterilised sawdust as bedding material and paper as cage-enrichment. Animals were acclimitised for a period of 5 days prior to exposure. The animals had free access to tap water and pelleted rodent diet (SM R/M-Z from SSNIFF® Spezialdiäten GmbH, Soest , Germany.
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- unchanged (no vehicle)
- Details on oral exposure:
- Animals were orally exposed to a single dose using plastic feeding tubes. The OECD guideline prescribes that the test substance should be administered in a single dose by gavage using a single dose. Therefore no vehicle was necessary.
- Doses:
- The dose level was 2000 mg/kg (1.98 ml.kg) body weight on day 1. This dose level was selected based on the acute oral toxicity of the closely structure-related dihydro isojasmonate, which is also > 2000 mg/kg.
- No. of animals per sex per dose:
- 3 female rates per group. Two groups of rats.
- Control animals:
- yes
- Details on study design:
- The toxicity of the test substance was assessed by stepwise treating the two groups of animals (group 1: 18 November 2010; group 2: 1 December 2010) at a dose level of 2000 mg/kg. The absence or presence of mortality deteremined the dose of the next step.
- Statistics:
- The method used is not intended to calculate a precise LD50, hence no statistical analysis was performed. The oral LD50 was ranked and an LD50 cut-off value determined based on the OECD 423 guideline.
Results and discussion
- Preliminary study:
- The structure-related compound dihydro isojasmonate (CAS 37172-53-5) is non-toxic. The test substance and dihydro isojasmonate both belong to the aliphatic ketones and differ by only on carbon in the alkyl group. The LD50 of the dihydro isojasmonate in 10 male Wistar rats exceeds 5000 mg/kg.
Effect levels
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 2 000 mg/kg bw
- Based on:
- test mat.
- Mortality:
- No mortality occurred.
- Clinical signs:
- All animals showed hunched posture on day 1. In addition, four out of 6 animals showed piloerection and/or abnormal gait on day 1 and hunched posture on day 2 (Table 1).
- Body weight:
- The body weight gain during the study period was similar to the group of untreated animals (Table 2).
- Gross pathology:
- No abnormalities were found at the macroscopic post mortem examinations of the animals. The scheduled necropsy was on day 15 after treatment.
Any other information on results incl. tables
Table 1 Clinical signs of the female Wistar rats treated with 2000mg/kg test substance.
Test Day | 1 | 1 | 1 | 2 | 3-15 | ||
hours after treatment | 0 | 2 | 4 | ||||
Animal 1 | |||||||
Hunched posture | - | 1 | 1 | 1 | - | ||
Abnormal gait | - | 1 | 1 | - | - | ||
Piloerection | - | 1 | 1 | - | - | ||
Animal 2 | - | 1 | 1 | - | - | ||
Hunched posture | - | 1 | 1 | 1 | - | ||
Abnormal gait | - | 1 | 1 | - | - | ||
Piloerection | - | 1 | 1 | - | - | ||
Animal 3 | - | 1 | 1 | - | - | ||
Hunched posture | - | 1 | 1 | 1 | - | ||
Abnormal gait | - | 1 | 1 | - | - | ||
Piloerection | - | 1 | 1 | - | - | ||
Animal 4 | - | 1 | 1 | - | - | ||
Hunched posture | 1 | 1 | 1 | 1 | - | ||
Piloerection | - | - | 1 | - | - | ||
Animal 5 | - | - | |||||
Hunched posture | 1 | 1 | 1 | - | - | ||
Animal 6 | - | 1 | 1 | - | - | ||
Hunched posture | 1 | 1 | 1 | - | - |
- : sign not observed; Hunched posture and piloerection: 1 is present; Abnormal gait grading slight (1) to severe (3);
Table 2 Body weight in grams of the female Wistar rats treated with 2000 mg/kg test substance
Day 1 | Day 8 | Day 15 | Day 1 | Day 8 | Day 15 | |||
Animal | Animal | |||||||
1 | 179 | 198 | 205 | 4 | 175 | 207 | 219 | |
2 | 176 | 201 | 211 | 5 | 174 | 205 | 219 | |
3 | 175 | 202 | 209 | 6 | 164 | 190 | 202 | |
mean | 177 | 200 | 208 | mean | 171 | 201 | 213 | |
std | 2 | 2 | 3 | std | 6 | 9 | 10 | |
n | 3 | 3 | 3 | n | 3 | 3 | 3 |
std: standard deviation; n: number of animals
Applicant's summary and conclusion
- Interpretation of results:
- study cannot be used for classification
- Remarks:
- Migrated information: based on GHS (2007) and Regulation (EC) 1272/2008.
- Conclusions:
- The oral LD50 of the test substance in Wistar rats exceeds 2000 mg/kg body weight. The LD50 cut-off value was considered to exceed 5000 mg/kg body weight.
- Executive summary:
The acute toxicity class method (OECD 423 (2001)) was used to assess the acute toxicity of test substance in 6 female Wistar rats.
The test substance was administered by oral gavage to two subsequent groups of rats at a dose level of 2000 mg/kg body weight. Animals were observed daily and their body weights were weekly recorded. Macroscopic examination was performed after sacrifice.
No mortality occurred. All animal showed hunched posture on day 1 and four out of six animals showed piloerection and/or abnormal gait on day 1 and hunched posture on day 2.
The body weight gain was normal.
No abnormalities were found at post mortem examinations of the animals.
The LD50 of the test substance exceeds 2000 mg/kg body weight and the LD50 cut-off value exceeds 5000 mg/kg body weight.
Consequently, the test substance does not need to be classified according to the GHS and Regulation (EC) 1272/2008.
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