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Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From Feb. 1, 1988 to Apr. 25, 1988
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Well documented, according to accepted guideline, with GLP-like Quality Assurance

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1988
Report Date:
1988

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
Deviations:
yes
Remarks:
The volume of the test solution was reduced from 3.0 l to 1.5 l. Blank CO2 evolution: 27.3 mg/1.5 l.
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Analytical purity: commercial grade
- Lot/batch No.: EN.116905.72
- Expiration date of the lot/batch: 11/92
- Storage condition of test material: room temperature

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Bacteria collected from activated sludge of the sewage treatment plant of CH - 4153 Reinach on 08/03/88. The preparation was carried out according to the method described in the guideline.
Duration of test (contact time):
28 d
Initial test substance concentrationopen allclose all
Initial conc.:
10 mg/L
Based on:
test mat.
Initial conc.:
20 mg/L
Based on:
test mat.
Initial conc.:
7.024 mg/L
Based on:
ThIC
Initial conc.:
14.048 mg/L
Based on:
ThIC
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
CO2 evolution
Details on study design:
TEST CONDITIONS
- Composition of medium: The test medium was prepared according to the method described in the guideline

- Test temperature: 22 ± 2°C

- Aeration of dilution water: ~ 25 ml/min free of carbon dioxide

TEST SYSTEM
- Culturing apparatus: 2 Liter flasks equipped with gas inlet and magnetic stirrer.
- Number of culture flasks/concentration: one

- Number of culture flasks/negative control: one

- Number of culture flasks/positive control: one
Reference substance
Reference substance:
aniline

Results and discussion

Preliminary study:
not reported
Test performance:
No unusual observations during test or any other information affecting results.
% Degradationopen allclose all
Parameter:
% degradation (CO2 evolution)
Value:
8
Sampling time:
28 d
Remarks on result:
other: 10 mg test substance/l
Parameter:
% degradation (CO2 evolution)
Value:
0
Sampling time:
28 d
Remarks on result:
other: 20 mg test substance/l

BOD5 / COD results

Results with reference substance:
97% in 28 days (20 mg/L reference substance)

Applicant's summary and conclusion

Interpretation of results:
other: not readily biodegradable according to OECD criteria