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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP study according to internationally accepted guideline. No deviations.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1992
Report Date:
1992

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
older version OECD guideline: Daphnia sp., Acute Immobilisation Test and Reproduction Test
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Lot/Batch number: 5 A 7990
Purity: 99.48%
Water solubility: 200 mg/l

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
HPLC analyses were performed at 0 and 48 h. Test solutions were analysed daily for pH, temperature and oxygen content

Test solutions

Vehicle:
yes
Details on test solutions:
Daily preparation of a stock solution containing 500 mg/l of substance and 0.01% of methylcellulose, used as solubility coadjuvant agent. Solution made with the aid of an ultrasonic device.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: Laboratory breeding
- Age at study initiation: less than 24 hours
- Method of breeding: no data
- Feeding during test: no
- Feed during breeding: S. capricornutum
- Amount: not stated
- Frequency: 3 times/week

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h

Test conditions

Hardness:
50 mg CaCO3/L
Test temperature:
22 °C
pH:
see table
Dissolved oxygen:
see table
Nominal and measured concentrations:
Nominal: 500 mg/L. HPLC analyses were performed at 0 and 48 h.
But no data of the results present in this study report.
Details on test conditions:
TEST SYSTEM
- Test vessel: no data
- Type (delete if not applicable): open
- Aeration: no
- No. of organisms per vessel: 5 animals/vessel
- No. of vessels per concentration (replicates): 4 vessels/concentration
- No. of vessels per vehicle control (replicates): 4 vessels water only + 4 vessels water + vehicle

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Tap water from the municipal main system.

OTHER TEST CONDITIONS
- Photoperiod: animals were ketp in the dark during test

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility

TEST CONCENTRATIONS
- Test concentrations: 500 mg/L (limit test)

Results and discussion

Effect concentrationsopen allclose all
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC100
Effect conc.:
> 500 mg/L
Nominal / measured:
nominal
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
500 mg/L
Nominal / measured:
nominal
Details on results:
No immobilisation observed.

Any other information on results incl. tables

Validity criteria for acute daphnia immobilisation test according to OECD Guideline 202.

 

fulfilled

Not fullfilled

Immobilisation of control animals <10%

YES

 

Control animals not staying at the surface

YES

 

Concentration of dissolved oxygen in all test vessels >3 mg/l

YES

 

Concentration of test substance³80% of initial concentration during test

YES

 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Remarks:
see above
Conclusions:
The test fulfilled the validity criteria. The test substance is not harmful to D. magna
Executive summary:

The acute toxicity of PAC in Daphnia magna was studied by means of a static test. 20 animals, divided over 4 replicates, were exposed to 500 mg/l PAC in aqueous 0.01% methylcellulose. Further 2 groups of 20 animals each one were exposed to water only or to the vehicle in water. The exposure lasted for 48 -hours. No immobility was observed. Therefore a concentration of 500 mg/L is a No Observed Effect Concentration.