Registration Dossier
Registration Dossier
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EC number: 214-406-2 | CAS number: 1125-21-9
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline Study. GLP. Scientifically acceptable.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 996
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2,6,6-trimethylcyclohex-2-ene-1,4-dione
- EC Number:
- 214-406-2
- EC Name:
- 2,6,6-trimethylcyclohex-2-ene-1,4-dione
- Cas Number:
- 1125-21-9
- Molecular formula:
- C9H12O2
- IUPAC Name:
- 2,6,6-trimethylcyclohex-2-ene-1,4-dione
- Details on test material:
- - Name of test material (as cited in study report): Oxoisophoron
- Analytical purity: > 98 %
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test solutions
- Vehicle:
- no
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Activated sludge taken from a WWTP treating municipal sewage.
- Initial biomass concentration: 1 g/l
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 30 min
Test conditions
- Test temperature:
- 20 +/- 2 °C
- Nominal and measured concentrations:
- Nominal test substance concentration: 1000 mg/l
- Details on test conditions:
- TEST SYSTEM
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3 - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 30 min
- Dose descriptor:
- EC0
- Effect conc.:
- >= 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC10
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 30 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 1 000 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
