2-ethoxyethylamine

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study in compliance with GLP-criteria.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 A (Ready Biodegradability: DOC Die Away Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.4-A (Determination of the "Ready" Biodegradability - Dissolved Organic Carbon (DOC) Die-Away Test)

Test guideline 3

Qualifier:

according to guideline

Guideline:

ISO 7827 (Evaluation in an Aqueous Medium of the "Ultimate" Aerobic Biodegradability of Organic Compounds - Method by Analysis of Dissolved Organic Carbon (DOC))

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-ethoxyethylamine

EC Number:

203-801-5

EC Name:

2-ethoxyethylamine

Cas Number:

110-76-9

Molecular formula:

C4H11NO

IUPAC Name:

2-ethoxyethylamine

Details on test material:

- Name of test material (as cited in study report): 2-Ethoxyethylamin
- Physical state: liquid
- TOC: 558 mg/g
- DOC: 555 mg/g

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

30 mg/L (dry matter content) from laboratory wastewater treatment plants with municipal and synthetic sewage.

Duration of test (contact time):

17 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

37.3 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

20 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Composition of medium: inorganic medium according to guideline
- Test temperature: ambient
- pH adjusted: no
- Continuous darkness: yes

TEST SYSTEM
- Culturing apparatus: Shaken flasks cultured under aerobic conditions and constant temperature
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: continuous aeration as prescribed by the guideline
- Measuring equipment: DOC analyser (Shimadzu TOC 500 and TOC 5000)
- Test performed in open system: yes

SAMPLING
- Sampling frequency: day 0, 1, 3, 5, 7, 11, 14, 16 and 17

CONTROL AND BLANK SYSTEM
- Inoculum blank: BC, 2 replicates
- Abiotic sterile control: PC, 1 replicate
- Toxicity control: RS, 1 replicate
- Other: inhibition control (IH, 1 replicate); adsorption control (AC, 1 replicate)

Reference substance

Reference substance:

aniline

Results and discussion

% Degradation

Parameter:

% degradation (DOC removal)

Value:

90 - 100

Sampling time:

17 d

Details on results:

- Degradation of 90-100 % was observed after 7 days.
- Adaptation phase: 5 days.
- Degradation phase: 6 days.
- Phys.-Chem. elimination (% DOC): <10 after 17 days.
- Elimination (adsorption) (% DOC): < 10 after 7 days.

Any other information on results incl. tables

DOC decrease (%)

Day	RS	IH	PC	AC	TS1	TS2	TSmean
0	0	0	0	0	0	0	0
1	4	6	13	9	-2	2	0
3	4	-6	-7	-3	2	3	3
5	60	47	-3	-7	0	-1	-1
7	94	54	-1	-4	94	82	88
11	96	96	1	-	94	96	95
14	101	97	-4	-	97	94	96
16	99	98	-4	-	101	98	100
17	102	101	-12	-	102	104	103