Registration Dossier

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Fish:

No mortality in either the control or the test groups was noted throughout the exposure period, therefore the EC50 could not be calculated. Under the conditions of the test, the 96 hour LC50 value for Lipase, batch PPW 21180 with rainbow trout was > 262.3 mg enzyme concentrate dry matter/L equivalent to 37.4 mg active enzyme protein (aep)/L.

The 'no-observed effect concentration' for Lipase, batch PPW 21180 with rainbow trout was 54.8 mg enzyme concentrate dry matte/L, equivalent to 7.8 mg aep/L.

The results are based on the nominal concentrations - which are the lower values - as a worse case.

 

Invertebrates:
Under the conditions of the test, Lipase, batch PPW 21180 was not found to be acutely toxic to Daphnia magna up to 262.3 mg enyzme concentrate dry matter/L (equivalent to 37.4 mg active enzyme protein/L) and thus no 48-hr EC50 value could be determined and must be over the highest tested concentration. NOEC was 262.3 mg enyzme concentrate dry matter/L (equivalent to 37.4 mg active enzyme protein/L). The results are based on the nominal concentrations - which are the lower values - as a worse case.

 

Algae:
Lipase, batch PPW 21180 did not inhibit the growth of Selenastrum capricornutum, at any concentration under the conditions of this test. The ErC50 (0 – 72 hours) was > 262.3 mg enzyme concentrate dry matter/L (equivalent to 37.4 mg active enzyme protein/L).

The EbC50 (72 hours) was > 262.3 mg enzyme concentrate dry matter/L. The No-observed-effect concentration NOEC was 262.3 mg enzyme concentrate dry matter/L (equivalent to 37.4 mg active enzyme protein/L). The results are based on the nominal concentrations - which are the lower values - as a worse case.

 

Lipase, batch PPW3983 is inhibitory to the growth of Desmodesmus subspicatus. NOEC was 38.1 mg enzyme concentrate dry matter/L (equivalent to 6.2 mg active enzyme protein/L). The 72h ErC50 is 94.2 mg enzyme concentrate dry matter/L (equivalent to 15.5 mg active enzyme protein/L) and the EbC50 is 92.3 mg enzyme concentrate dry matter/L.

Additional information

The lowest EC50 value (of algae), 94.2 mg enzyme concentrate dry matter/L (equivalent to 15.5 mg active enzyme protein/L) was used in PNEC calculations.