Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 696-364-9 | CAS number: 133779-11-0
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The 21d EC50 daphnia magna of 566 µg/L (parental mortality, OECD 211, river water test, reliability 1) is used as key value for the acute toxicity to aquatic invertebrates in the chemical safety assessment.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 566 µg/L
Additional information
Acute toxicity tests are available for daphnia magna for various alkyl-1,3 -diamines with C12 -18 chains (reliability 2 and 3).
The lowest 48h EC50 for Daphnia magna of 6.2 ug/L was determined in a test with the glassware presoaked overnight. At that time it was thought that this approach would compensate the loss of the substance to glassware. Later on the use of LC-MS/MS techniques to quantify the substance down to 0.5 µg/L, showed that presaturation is leading to test concentrations which are higher than nominal. Therefore this value has not been selected as key value.
The acute toxicity tests were conducted in a period when no reliable specific method of analyses was available. The concentrations were therefore not analytically verified. The substance is known for its poor water solubility and the strong tendency to adsorb onto the walls of test vessels. Therefore it was decided to perform a number of higher tier chronic invertebrate studies with analytical monitoring of the test concentrations. These higher tier tests were performed in river water to allow a PECaquatic,bulk/PNECaquatic,bulk approach for the environmental risk assessment. The long term 21d EC50 for parental mortality determined in the OECD211 test with river water (reliability 1) was used as the key value for acute toxicity for daphnia in the chemical safety assessment.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
