Registration Dossier
Registration Dossier
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EC number: 204-682-2 | CAS number: 124-12-9
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study under GLP with minor deviations.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 1996
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- yes
- Principles of method if other than guideline:
- Reference substance: copper(II)sulfate-pentahydrate instead of 3,5-dichlorophenol due to carciogenic properties
Sludge was washed 2x instead of 3x. This has been proved to be sufficient.
Dry weight of sludge: 3g/l±20% instead of 4g/l±10% due to high activity of activated sludge from sewage plant Hildesheim. - GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Octanenitrile
- EC Number:
- 204-682-2
- EC Name:
- Octanenitrile
- Cas Number:
- 124-12-9
- Molecular formula:
- C8H15N
- IUPAC Name:
- octanenitrile
Constituent 1
Sampling and analysis
- Details on sampling:
- Ultrasound dispersing: 30 min at 40°C.
Test concentrations: 10 - 32 - 100 - 320 - 1000 [mg/L]
Single stock solution. No carrier was used.
Test solutions
- Details on test solutions:
- Ultrasound dispersing: 30 min at 40°C.
Test concentrations: 10 - 32 - 100 - 320 - 1000 [mg/L]
Single stock solution. No carrier was used.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Origin: domestic sewage plant Hildesheim, clarifier
washed two times with dechlorinated tap water
Sludge dry weight: 3,0 [g/L]
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
Test conditions
- Test temperature:
- 20°C
- pH:
- 7,11
- Nominal and measured concentrations:
- 10 - 32 - 100 - 320 - 1000 [mg/L]
- Details on test conditions:
- The respiration rates were determined after 3-hour contact time aby determination of the inhibitory effect of the test concentrations compared to the control system.
Concentrations: 10 - 32 - 100 - 320 - 1000 [mg/L].
Concentrations of referece substance: 58 - 100 - 180 [mg/L] - Reference substance (positive control):
- yes
- Remarks:
- Copper( Il)sulfate-pentahydrate p.a. (>99%)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 169 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Remarks on result:
- other: 11-1000
- Duration:
- 3 h
- Dose descriptor:
- other: EC20
- Effect conc.:
- 34 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 3 h
- Dose descriptor:
- other: EC80
- Effect conc.:
- 852 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- EC50 of reference substance = 99 [mg/L]
- Reported statistics and error estimates:
- The deviation of control respiratory rates was 3 [%].
Any other information on results incl. tables
| concentration (mg/L) | respiratory rates [mg 02/L h] | inhibition (%) | |
| Substance | 1000 | 5.6 |
84 |
| 320 | 2.8 | 64 | |
| 100 | 24.0 | 32 | |
| 32 | 28.4 | 20 | |
| 10 | 32.4 | 8 | |
| Control K1 | 0 | 36.0 | 0 |
| Control K2 | 0 | 34.8 | 0 |
| Reference substance | 180 | 6.4 | 80 |
| 100 | 15.6 | 51 | |
| 58 | 24.8 | 22 | |
| Control K1 | 0 | 30.0 | 0 |
| Control K2 | 0 | 33.6 | 0 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance shows in concentrations <34 [mg / L] no toxic effect to bacteria in activated sludge of a municipal wastewater treatment plant. The EC50 was determined to be EC50=169mg/l.
- Executive summary:
An activated sludge, respiratory inhibition test according to OECD209 guideline was performed under GLP. The test was performed with domestic activated sludge over a period of 3h.
The substance shows in concentrations <34 [mg / L] no toxic effect to bacteria in activated sludge of a municipal wastewater treatment plant. The EC50 was determined to be EC50=169mg/l.
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