Fatty acids, C18-unsatd., trimers, 2-ethylhexyl esters

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

29 Apr - 13 May 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1997

Report date:

1997

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

Version / remarks:

(adopted in 1996)

Deviations:

no

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

2-ethylhexyl 8-[7,8-bis({8-[(2-ethylhexyl)oxy]-8-oxooctyl})-4,5-dihexyl-6-[(1E)-oct-1-en-1-yl]-1,2,5,6,7,8-hexahydronaphthalen-1-yl]octanoate; 8-[1,8-bis({8-[(2-ethylhexyl)oxy]-8-oxooctyl})-4,5-dihexyl-3-[(1E)-oct-1-en-1-yl]-1,2,3,4,7,8-hexahydronaphthalen-2-yl]octanoic acid; 8-[2,8-bis({8-[(2-ethylhexyl)oxy]-8-oxooctyl})-4,5-dihexyl-3-[(1E)-oct-1-en-1-yl]-1,2,3,4,7,8-hexahydronaphthalen-1-yl]octanoic acid; 8-[7,8-bis({8-[(2-ethylhexyl)oxy]-8-oxooctyl})-4,5-dihexyl-6-[(1E)-oct-1-en-1-yl]-1,2,5,6,7,8-hexahydronaphthalen-1-yl]octanoic acid

EC Number:

605-694-4

Cas Number:

173832-46-7

Molecular formula:

uvcb not available

IUPAC Name:

2-ethylhexyl 8-[7,8-bis({8-[(2-ethylhexyl)oxy]-8-oxooctyl})-4,5-dihexyl-6-[(1E)-oct-1-en-1-yl]-1,2,5,6,7,8-hexahydronaphthalen-1-yl]octanoate; 8-[1,8-bis({8-[(2-ethylhexyl)oxy]-8-oxooctyl})-4,5-dihexyl-3-[(1E)-oct-1-en-1-yl]-1,2,3,4,7,8-hexahydronaphthalen-2-yl]octanoic acid; 8-[2,8-bis({8-[(2-ethylhexyl)oxy]-8-oxooctyl})-4,5-dihexyl-3-[(1E)-oct-1-en-1-yl]-1,2,3,4,7,8-hexahydronaphthalen-1-yl]octanoic acid; 8-[7,8-bis({8-[(2-ethylhexyl)oxy]-8-oxooctyl})-4,5-dihexyl-6-[(1E)-oct-1-en-1-yl]-1,2,5,6,7,8-hexahydronaphthalen-1-yl]octanoic acid

Details on test material:

- Name of test material (as cited in study report): [trade name]
- Physical state/appearance: brown liquid
- Analytical purity: no data
- Batch No.: N558331
- Densitiy: 930 kg/m³
- Storage: room temperature, in the dark

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Strain as cited in study report: Wistar strain Crl:(WI) BR (outbred, SPF-Quality)
- Source: Charles River, Sulzfeld, Germany
- Age at study initiation: approx. 8 weeks old
- Mean weight at study initiation: 267 g (males); 177 g (females)
- Fasting period before study: yes (overnight)
- Housing: 3 per cage in polycarbonate cages
- Diet: standard pelleted laboratory animal diet (Carfil Quality BVBA, Out-Turnhout, Belgium); ad libitum
- Water: tap water; ad libitum
- Acclimation period: at least 5 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21
- Humidity (%): 50
- Air changes (per hr): approx. 15
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Details on oral exposure:

Animals were fasted overnight prior to dosing until approx. 3-4 hours after administration of the test substance.

Doses:

2000 mg/kg bw (2.15 mL/kg bw)
(Dose volume calculated as follows: dose level (g/kg) : densitiy (0.93 g/mL))

No. of animals per sex per dose:

- Group 1: 3 males
- Group 2: 3 females

Control animals:

no

Details on study design:

- Duration of observation period following administration: 15 days
- Frequency of observations: daily
- Frequency of weighing: day 1 (pre-administration), day 8 and day 15
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight

Statistics:

No statistical analysis was performed.

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: LD50 experimental

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 5 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: LD50 cut-off according to OECD 423

Mortality:

No mortality occurred.

Clinical signs:

other: No clinical signs were observed during the study period.

Gross pathology:

No abnormalities were found at macroscopic post mortem examination of the animals.

Applicant's summary and conclusion

Interpretation of results:

not classified

Remarks:

Migrated information Criteria used for interpretation of results: EU

Conclusions:

CLP: not classified
DSD: not classified