Registration Dossier
Registration Dossier
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EC number: 235-185-9 | CAS number: 12125-01-8
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Ammonium toxicity: A 28-day study on Pimephales promelas embryos with ammonium chloride is considered as a key study.
Fluoride toxicity: A 21-day study in Oncoryhychus mykiss with sodium fluoride is reviewed in the EU RAR and fluoride ICD (RIVM).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 4 mg/L
Additional information
As ammonium fluoride decomposes in an aqueous environment into ammonium and fluoride ions, toxicity of these two ions has been studied and the most conservative one has been kept for assessment.
Ammonium toxicity was studied in a chronic toxicity test, in which Pimephales promelas embryos were exposed to various concentrations of ammonium chloride under flow through conditions. The methodology followed was ASTM E729-8 (similar to OECD 212 guideline). The result obtained is a 28d NOEC of 0.17 mg/L (expressed as NH3-N) and recalculated this is 11.8 mg/L of NH4Cl.
Fluoride toxicity was studied in a 21 day test with Oncorhynchus mykiss, a LC5 value of 4 mg/L was reported (actual concentration). This value is considered to be equivalent to the NOEC for mortality. The test was conducted in very soft (12 mg CaCO3/L) natural water with daily renewal of the test water.
In conclusion, considering the respective toxicity to fish, the most conservative NOEC for fluoride is retained as key data for hazard assessment.
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