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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1983
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Endpoint only value. The document is insufficient for assessment
Cross-reference
Reason / purpose for cross-reference:
reference to same study

Data source

Reference
Reference Type:
secondary source
Title:
Unnamed
Year:
1983

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Deviations:
not specified
Principles of method if other than guideline:
Not applicable
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Sodium 2-chloropropionate
EC Number:
241-067-8
EC Name:
Sodium 2-chloropropionate
Cas Number:
16987-02-3
Molecular formula:
C3H5ClO2.Na
IUPAC Name:
sodium 2-chloropropanoate
Details on test material:
The test was conducted with the sodium salt of 2-chloropropionic acid, CAS number 16987-02-3, EC number 241-067-8

Method

Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Details on mammalian cell type (if applicable):
No additional data presented
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Test concentrations with justification for top dose:
20 µg to 500 µg per plate
Vehicle / solvent:
Not available
Controls
Untreated negative controls:
not specified
Negative solvent / vehicle controls:
not specified
True negative controls:
not specified
Positive controls:
not specified
Positive control substance:
not specified
Details on test system and experimental conditions:
Standard plate and preincubation test
Evaluation criteria:
No additional data presented
Statistics:
No additional data presented

Results and discussion

Test results
Key result
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
not specified
Untreated negative controls validity:
not specified
True negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
No additional data presented

Any other information on results incl. tables

None

Applicant's summary and conclusion

Conclusions:
Interpretation of results:
negative

A negative result was obtained in an Ames test conducted on the sodium salt of 2-chloropropionic acid.
Executive summary:

The sodium salt of 2-chloropropionic acid was subject to an Ames test according to OECD guideline 471. The test was carried out according to GLP. A negative result was obtained both with and without metabolic activation.