Vinasses, residue of fermentation containing biomass of Corynebacterium glutamicum

Density

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

bulk density

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Principles of method if other than guideline:

VDLUFA, determination of volume, weighing

GLP compliance:

not specified

Type of method:

other: determination of volume, weighing

Type:

bulk density

Density:

>= 0.77 - <= 1.23 g/cm³

Remarks on result:

other: Temperature was not reported

Temperature was not reported.

Reference 2

Endpoint:

relative density

Type of information:

experimental study

Adequacy of study:

key study

Study period:

28 Jun - 27 Aug 2010

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 109 (Density of Liquids and Solids)

Deviations:

yes

Remarks:

: The mean volume of the test substance determined by the gas comparison pycnometer was with n=3 instead of n=4. The study integrity was not adversely affected by the deviations.

Qualifier:

according to guideline

Guideline:

EU Method A.3 (Relative Density)

Deviations:

yes

Remarks:

: The mean volume of the test substance determined by the gas comparison pycnometer was with n=3 instead of n=4. The study integrity was not adversely affected by the deviations.

Principles of method if other than guideline:

The mean volume of the test substance determined by the gas comparison pycnometer was with n=3 instead of n=4.
Evaluation: With the test substance at both tests, 3 instead of 4 accurate measurements on the volume were obtained. The values for the density calculated with the mean volumes were within the criterion range of < 0.02 g/cm3. Based on this it was considered that the mean volumes obtained were accurate for the determination of the density of the test substance.

The study integrity was not adversely affected by the deviations.

GLP compliance:

yes (incl. QA statement)

Remarks:

Food and Consumer Product Safety Authority, Prinses Beatrixlaan 2, 2595 AL Den Haag, Postbus 19506, 2500 CM Den Haag, The Netherlands, 30 March 2009

Type of method:

air comparison pycnometer (for solids)

Type:

density

Density:

1.53 g/cm³

Temp.:

20 °C

Remarks on result:

other: mean of 2 tests

Type:

relative density

Density:

1.53

Temp.:

20 °C

Performance of the study

The density and relative density of the test substance were determined using a gas comparison stereopycnometer (Pycnomatic, Porotec, Frankfurt, Germany). With this method the volume of the test substance is measured in helium gas (Air Products, Amsterdam, The Netherlands) in a calibrated cylinder of variable volume. After concluding the volume measurement, the mass of the sample is determined and the density calculated.

                       

The test substance was reduced by grinding using a mortar and dried in a desiccator under vacuum using silicagel as the drying agent at 20.2 ± 0.6°C in the dark for 44 hours.

 

The sample cell was filled with ground test substance, weighed and placed in the gas comparison pycnometer. The mean volume of the test substance was determined (n = 3). Thereafter the sample cell was weighed for the determination of the mass of the test substance. With this information, the density of the test substance was calculated. The test was performed in duplicate. The mean density and relative density of the test substance were calculated.

 

Handlings were performed at 20.0 ± 0.5°C. Weighing was performed on an analytical balance with an accuracy of 0.1 mg.

 

Calibrations       

On the day of measurement the validity of the gas comparison pycnometer was verified using a calibration ball with a known diameter of 31 mm ± 1.1 μm (calibrated by Kalibra International, Delft, The Netherlands). The reference volume, sample chamber volume and vessel volume of the system were determined. The values obtained were within the NOTOX criteria range. From this it was possible to apply the system for the determination of the density of the test substance.

 

Reference 3

Endpoint:

density, other

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

other: Only short summary available.

Guideline:

other: no data

Principles of method if other than guideline:

Data taken from Material Safety Data Sheet.

GLP compliance:

not specified

Type:

density

Density:

ca. 1.3 other: kg/L

Temp.:

20 °C

Description of key information

1) 1.53 g/cm³ at 20 °C (solid substance)
2) Range of bulk density: 0.77 - 1.23 g/cm³

Additional information

1) OECD Guideline 109, air comparison pycnometer

2) Determination of volume, weighing