Tris(methylphenyl) phosphite

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05FEB2008 to 07FEB2008

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Guideline study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Tris(methylphenyl) phosphite

EC Number:

247-119-6

EC Name:

Tris(methylphenyl) phosphite

Cas Number:

25586-42-9

Molecular formula:

C21H21O3P

IUPAC Name:

tris(methylphenyl) phosphite

Constituent 2

Reference substance name:

Tris(methylphenyl) phosphate

EC Number:

215-548-8

EC Name:

Tris(methylphenyl) phosphate

Cas Number:

1330-78-5

IUPAC Name:

tris(4-methylphenyl) phosphate

Details on test material:

- Name of test material (as cited in study report): Tritolyl phosphite (BU)
- Analytical purity: 91.8%
- Lot/batch No.: 11237993-7079
- Expiration date of the lot/batch: 23-November-2008
- Storage condition of test material: The test item was stored at room temperature (approx. 20 °C) in the dark.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

- Age at study initiation (mean and range, SD): 6-24 hours

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20-21 °C during the test period

pH:

7.3-7.8

Dissolved oxygen:

7.6-8.5 mg/L

Nominal and measured concentrations:

Nominal concentrations: 3.2, 10, 32 and 100 mg/L

Details on test conditions:

A 16-hour light to 8-hour dark photoperiod, light intensity during the light period was between 200 and 1200 Lux

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Table 1: Effect of Tritolyl Phosphite (BU) on the mobility ofDaphnia magna,Test Part I
Loading rate	No. of daphnids tested	Immobilized daphnids after 24 hours		Immobilized daphnids after 48 hours
mg/L		No.	%	No.	%
Control	20	0	0	0	0
3.2	20	0	0	0	0
10	20	0	0	0	0
100	20	0	0	1	5
EC50 (mg/L)		>100		>100
95% confidence limits		Could not be determined		Could not be determined

Table 1: Effect of Tritolyl Phosphite (BU) on the mobility ofDaphnia magna,Test Part II
Loading rate	No. of daphnids tested	Immobilized daphnids after 24 hours		Immobilized daphnids after 48 hours
mg/L		No.	%	No.	%
Control	20	0	0	0	0
3.2	20	0	0	0	0
10	20	0	0	0	0
100	20	0	0	0	0
EC50 (mg/L)		>100		>100
95% confidence limits		Could not be determined		Could not be determined