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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
05FEB2008 to 07FEB2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2008
Report date:
2008

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Qualifier:
according to guideline
Guideline:
EU Method C.2 (Acute Toxicity for Daphnia)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Tris(methylphenyl) phosphite
EC Number:
247-119-6
EC Name:
Tris(methylphenyl) phosphite
Cas Number:
25586-42-9
Molecular formula:
C21H21O3P
IUPAC Name:
tris(methylphenyl) phosphite
Constituent 2
Reference substance name:
Tris(methylphenyl) phosphate
EC Number:
215-548-8
EC Name:
Tris(methylphenyl) phosphate
Cas Number:
1330-78-5
IUPAC Name:
tris(4-methylphenyl) phosphate
Details on test material:
- Name of test material (as cited in study report): Tritolyl phosphite (BU)
- Analytical purity: 91.8%
- Lot/batch No.: 11237993-7079
- Expiration date of the lot/batch: 23-November-2008
- Storage condition of test material: The test item was stored at room temperature (approx. 20 °C) in the dark.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
- Age at study initiation (mean and range, SD): 6-24 hours

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20-21 °C during the test period
pH:
7.3-7.8
Dissolved oxygen:
7.6-8.5 mg/L
Nominal and measured concentrations:
Nominal concentrations: 3.2, 10, 32 and 100 mg/L
Details on test conditions:
A 16-hour light to 8-hour dark photoperiod, light intensity during the light period was between 200 and 1200 Lux
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

Table 1: Effect of Tritolyl Phosphite (BU) on the mobility ofDaphnia magna,Test Part I

Loading rate

 

No. of

daphnids

tested

 

Immobilized

daphnids after

24 hours

 

Immobilized

daphnids after

48 hours

mg/L

No.

%

No.

%

Control

20

0

0

0

0

3.2

20

0

0

0

0

10

20

0

0

0

0

100

20

0

0

1

5

EC50 (mg/L)

>100

>100

95% confidence limits

Could not be determined

Could not be determined

Table 1: Effect of Tritolyl Phosphite (BU) on the mobility ofDaphnia magna,Test Part II

Loading rate

 

No. of

daphnids

tested

 

Immobilized

daphnids after

24 hours

 

Immobilized

daphnids after

48 hours

mg/L

No.

%

No.

%

Control

20

0

0

0

0

3.2

20

0

0

0

0

10

20

0

0

0

0

100

20

0

0

0

0

EC50 (mg/L)

>100

>100

95% confidence limits

Could not be determined

Could not be determined

Applicant's summary and conclusion