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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
-
EC Number:
413-890-4
EC Name:
-
Cas Number:
82469-79-2
Molecular formula:
C28H50O8
IUPAC Name:
1,2,3-trihexyl 2-(butanoyloxy)propane-1,2,3-tricarboxylate

Method

Species / strain
Species / strain / cell type:
bacteria, other: Salmonella typhimurium TA 98, TA 100, TA 1535, TA 1538 strains Escherichia coli strain WP2 uvra (WP2A)
Metabolic activation system:
Arochlor 1254 induced S-9 Mix from male SD rats.
Test concentrations with justification for top dose:
Concentration range in the main test (with metabolic activation): 667 ... 10000 µg/plate
Concentration range in the main test (without metabolic activation): 667 ... 10000 µg/plate
Vehicle / solvent:
Solvent: ethanol (100%)
Details on test system and experimental conditions:
Concentration of the test substance resulting in precipitation: 667 µg/plate

Results and discussion

Test resultsopen allclose all
Species / strain:
other: S. typhimurium TA98, TA100, TA1535, TA1537, TA1538
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not determined
Vehicle controls validity:
valid
Positive controls validity:
valid
Species / strain:
E. coli WP2 uvr A
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
not determined
Vehicle controls validity:
valid
Positive controls validity:
valid
Additional information on results:
Observations:
No positive responses seen with any of the tester strains in
the presence or absence of microsomal enzymes.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation

Citroflex B-6 did not cause a positive response with any tester strains in the presence and absence of microsomal enzymes prepared from Arochlor-induced rat liver.