Registration Dossier
Registration Dossier
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EC number: 423-870-7 | CAS number: -
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- October 2001 till January 2002
- Reliability:
- 1 (reliable without restriction)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- Version / remarks:
- , 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- ,signed 1999-05-25
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of activated sludge: Neustadt, (ESN), Im Altenschemel, NW-Lachen-Speyerdorf, Germany
- Charge: 18102001
- Preparation of inoculum for exposure: The activated sludge was washed 3 x with drinking water, filtered, washed again and resuspended in medium and ventilated for 12 h.
- Concentration of sludge: the dry residue was 4840 mg solid / L. - Duration of test (contact time):
- 29 d
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
Stock solution (a)-(d) were prepared as described in OECD 301 (1992)
- Composition of medium: 30 mL (a), 3 mL (b), 3 mL (c), 3 mL (d) filled with aqua demin. ad up to 1000 mL
- Test temperature: 20 +-2°C
- pH adjusted: no
- Aeration of dilution water: yes
- Suspended solids concentration: 25.8 mg/L
- Continuous darkness: without direct lighting
TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration: double determination
Determination of TC and IC
SAMPLING
- Sampling frequency: 1, 3, 6, 8, 10, 13, 16, 20, 24, 28 , 29
CONTROL AND BLANK SYSTEM
- Reference substance: Aniline
- Inoculum blank: 2x
- Abiotic sterile control: 1x (HgCl2 - 1.5 g)
- Toxicity control: 1x (Testsubstance and Aniline) - Reference substance:
- aniline
- Key result
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 88
- Sampling time:
- 28 d
- Details on results:
- The IC value of the reference item measured at day 24 is considered as an outlier (based on dilution)
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The test item is readily biodegradable since a 88% degradation was observed after 28 days (10-day window passed). A toxicity control was performed.
Reference
Description of key information
readily biodegradable (88 % after 28 days; 10-d window passed)
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
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