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EC number: 249-528-5 | CAS number: 29232-93-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 30 Jan 1978 to 3 Feb 1978
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study with acceptable restrictions
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The acute toxicity of the test substance to rainbow trout of was determined in freshwater at 12°C at for 96 hours. LC50 values and 95% confidence intervals were derived by probit analysis.
- GLP compliance:
- no
- Analytical monitoring:
- yes
- Details on sampling:
- Analytical samples were taken after 24, 48, 72 and 96 hour exposure. Samples and standards held at 4 °C in a refrigerator are stable for at least 24 hours.
- Vehicle:
- yes
- Remarks:
- Ethanol
- Details on test solutions:
- 10.00 g of the test substance was weighed and dissolved in 95% purity ethanol. The ethanol concentration in each exposure vessel was adjusted to 2000 mg/L. The dilution ratio of the stock concentrates to freshwater was 1:190 in this study.
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- TEST ORGANISM
- Common name: Rainbow trout
- Source: Samaaki Trout Farm, Wiltshire.
- Acclimation period: 1 day
- Holding condition: Temperature 8.5°C in stock ranks prior testing and in the test vessel at a temperature of 12°C-0.5°C.
- Weight of control fish: The fish ranged in weight from 5.10g - 10.59g with a mean weight of 7.49g.
- Length of control fish: The range in length was 77 to 104 mm with a mean length of 86 mm. - Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Hardness:
- Series I and II: 30-32 mg/L as CaCO3
Series III: 40-53 mg/L as CaCO3 - Test temperature:
- 12°C - 0.5°C
- pH:
- 7.5 to 7.65
- Dissolved oxygen:
- 87-96%
- Nominal and measured concentrations:
- - Nominal concentration: 0.18, 0.32, 0.42, 0.56, 0.75, 1.0, 2.4 and 5.6 mg/L
- Mean measured concentrations: 0.18, 0.306, 0.33, 0.51, 0.56, 0.74, 2.3, NA mg/L, respectively - Details on test conditions:
- TEST SYSTEM
- Test vessel: Twenty liter glass vessels
- Type: Open
- Type of flow-through: The concentrated stock solutions were fed to the system by a series of peristaltic pumps and a further series of these pumps was used to supply freshwater. The concentrated solutions of the test substance and freshwater were fed to glass splash heads where mixing occurred before the test solution p assed int-o the fish test vessels.
- No. of organisms per vessel: 20
- No. of vessels per concentration: 1
- No. of vessels per control: 1 vessel for the freshwater only control
- No. of vessels per vehicle control: 3 vessels for the solvent control
- Biomass loading rate: Not reported
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Distilled water
- Intervals of water quality measurement: Daily
OTHER TEST CONDITIONS
- Photoperiod: Not reported
- Light intensity: Not reported
EFFECT PARAMETERS MEASURED: Mortality
The mortalities of the fish in each concentration tested were recorded at 24, 48, 72 and 96 hours. - Reference substance (positive control):
- no
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 0.404 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality (fish)
- Details on results:
- Overview of the results are provided in the 'Any other information on results incl. tables'.
There were no mortalities at the lowest concentration tested, 0.18 mg/L of the test substance as the fish did not exhibit any toxic symptoms at 96 hours, this may be regarded as a no effect level under the test conditions. After 96 hours, no mortality was observed in any of these controls. Atthe lowest test concentration of 0.18 mg/L fish did not exhibit any toxic symptoms at 96 hours.The mortality increased to 30% in 0.32 mg/L and 100% to 0.75mg/L after 96h exposure. Based on these findings, the LC50 was determined to be 0.404 mg/L with 95% confidence limits of 0.364 to 0.448 mg/L. - Reported statistics and error estimates:
- 24, 48, 72 and 96 hour LC 50 values and 95% confidence intervals were determined by probit analysis.
- Sublethal observations / clinical signs:
Table 1. Concentrations tested and corresponding observed percentage moratilities for the rainbow trout
Nominal concentration mg/L test substance
Mortality observed
%
24 hours
48
hours
72
hours
96
hours
5.6
2.4
1.0
0.75
0.56
o.42
0.32
0.18
100
100
80
45
10
0
10
0
100
100
95
85
50
15
20
0
100
100
100
100
85
30
30
0
100
100
100
100
90
40
30
0
*Control SeriesI
*Control SeriesII
*Control SeriesIII
0
0
0
0
0
0
0
0
0
0
0
0
Freshwateronly
Control series III
0
0
0
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The LC50 value as determined to be 0.404 mg/L test substance corresponding 95% confidence limits of 0.364 to 0.448 mg/L.
- Executive summary:
The acute toxicity of the test substance to rainbow trout (Oncorhynchus mykiss) was studied in a 96-hour flow-through test in freshwater at 12°C.The study did not follow established guidelines and was not in compliance with GLP. Still, the study followed the correct study principles and thetest procedures and results are well documented. The study can therefore be considered reliable (with restrictions) and fully valid for the hazard and risk characterisation.In this study, the fish (twenty organisms per concentration) were exposed to nominal concentrations of 5.6, 2.4, 1.0, 0.75, 0.56, 0.42, 0.32 and 0.18 mg/L (mean measured concentrations were determined to be NA,2.3, 0.74, 0.56, 0.51, 0.33, 0.306 and 0.18 mg/L, respectively). The mortalities of the fish in each concentration tested were recorded at 24, 48, 72 and 96 hours. A blank control and a solvent control (containing 2000 ppm of ethanol) were also tested with each series.
After 96 hours, no mortality was observed in any of these controls. Atthe lowest test concentration of 0.18 mg/L fish did not exhibit any toxic symptoms at 96 hours.The mortality increased to 30% in 0.32 mg/L and 100% to 0.75mg/L after 96h exposure. Based on these findings, the LC50 was determined to be 0.404 mg/L with 95% confidence limits of 0.364 to 0.448 mg/L.
Reference
Description of key information
All available data was assessed and the study representing the worst-case effects are included here as key. The results can be considered worst-case and are selected for the CSA.
96-h LC50 = 0.404 mg/L, Oncorhynchus mykiss, flow-through regime, no guideline followed, Hill 1978
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 0.404 mg/L
Additional information
The acute toxicity of the test substance to rainbow trout (Oncorhynchus mykiss) was studied in a 96-hour flow-through test in freshwater at 12°C. The study did not follow established guidelines and was not in compliance with GLP. Still, the study followed the correct study principles and the test procedures and results are well documented. The study can therefore be considered reliable (with restrictions) and fully valid for the hazard and risk characterisation. In this study, the fish (twenty organisms per concentration) were exposed to nominal concentrations of 5.6, 2.4, 1.0, 0.75, 0.56, 0.42, 0.32 and 0.18 mg/L (mean measured concentrations were determined to be NA, 2.3, 0.74, 0.56, 0.51, 0.33, 0.306 and 0.18 mg/L, respectively). The mortalities of the fish in each concentration tested were recorded at 24, 48, 72 and 96 hours. A blank control and a solvent control (containing 2000 ppm of ethanol) were also tested with each series.
After 96 hours, no mortality was observed in any of these controls. At the lowest test concentration of 0.18 mg/L fish did not exhibit any toxic symptoms at 96 hours. The mortality increased to 30% in 0.32 mg/L and 100% to 0.75 mg/L after 96h exposure. Based on these findings, the LC50 was determined to be 0.404 mg/L with 95% confidence limits of 0.364 to 0.448 mg/L.
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