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EC number: 249-528-5 | CAS number: 29232-93-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to microorganisms, other
- Remarks:
- Pseudomonas growth inhibition test (Bringmann and Kühn; 1980)
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 4 Nov 1988 to 8 Nov 1988
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- other: Bringmann G and Kühn R (1980): Comparison of the toxicity thresholds of water pollutants to bacteria, algae and protozoa in the cell multiplication inhibition test. Water Research 14, 231-241
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- The flasks were determined after 6 hours exposure to the test substance.
- Vehicle:
- no
- Details on test solutions:
- Deionised water was added to 5 mg of the test substance to give a total volume of 1 L. This was stirred in the dark for 3 days and then allowed to stand in the dark for a further day. Stock solution of 5 mg/L test substance was used for the preparation of the test exposure solution.
- Test organisms (species):
- Pseudomonas putida
- Details on inoculum:
- - Test organism: Pseudomonas putida, strain NCIB9494, was obtained as a freeze-dried culture from National Collections of Industrial and Marine Bacteria Ltd, Aberdeen, UK. This was stored at 4°C until use. The freeze-dried culture was rehydrated in 0-5 mL of nutrient broth - A loop of this suspension was streaked onto a nutrient agar slope in a universal bottle - This was incubated at 25°C for 24 hours, and then stored at 4°C , until use as the stock culture
- Preparation of inoculum: 18-20 hours before the start of the test 4 ml of test medium concentrate were added to 46 ml of deionised water in a sterile conical flask. A loop of Pseudomonas putida stock culture was added to this growth medium solution, and then incubated overnight on an orbital shaker (150 rpm). After this period the cells were diluted by addition of fresh growth medium solution at 25 ° C to an optical density was measured. This was used as the test inoculum. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 6 h
- Test temperature:
- 25 °C
- pH:
- Not reported
- Dissolved oxygen:
- Not reported
- Salinity:
- Not applicable
- Conductivity:
- Not reported
- Nominal and measured concentrations:
- Nominal concentrations: 1 and 4.5 mg/L of the test substance. See Table 1 in 'Any other information on materials and methods incl. tables'.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: Flasks
- Type: Closed
- Fill volume: 50 mL
- No. of vessels per concentration (replicates): 2
- No. of vessels per control : 3
- No. of vessels per reference control (replicates): 2
- No. of vessels without inoculum (replicates): 2
- No. of vessels per blank control: 1
TEST MEDIUM
A basal salt solution was prepared by dissolving
- 26.25 g dipotassium hydrogen phosphate,
- 11.25 g potassium dihydrogen phosphate,
- 1.175 g trisodium citrate dihydrate,
- 2.5 g ammonium sulphate and
- 0.25 g magnesium sulphate heptahydrate
in deionised water and making up to 1 litre with deionised water. This solution was autoclaved at 121°C for 15 minutes. A glucose solution was prepared by dissolving 6.25 g glucose in deionised water and making up to 1 L with deionised water solution. This was autoclaved at 121°C for 15 minutes. Equal volumes of the salt solution and the glucose solution were mixed together in a sterile flask to form a growth medium concentrate. This was stored at 4°C until use.
See Table 1 in 'Any other information inmaterial incl. tables' for an overview of the results - Reference substance (positive control):
- yes
- Remarks:
- The reference substance was 3,5 dichlorophenol (99%)
- Key result
- Duration:
- 6 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 4.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Key result
- Duration:
- 6 h
- Dose descriptor:
- NOEC
- Effect conc.:
- >= 4.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Duration:
- 6 h
- Dose descriptor:
- EC10
- Effect conc.:
- > 4.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth inhibition
- Details on results:
- 0% inhibition was found at a concentration of 1 and 4.5 mg/L of the test substance after 6h exposure. It was not possible to test at higher concentrations because of the low water solubility of the substance. See Table 1 in "Any other information in results incl. tables" for an overview of the results.
- Results with reference substance (positive control):
- 94% growth inhibition was found at a concentration of 18 mg/L the reference substance dichlorophenol indicating that the Pseudomonas putida
culture was responding normally to this known toxicant. - Conclusions:
- Based on the nominal concentrations, the EC50 and EC10 of the test substance were graphically determined to be >4.5 mg/L. The NOEC was determined to be ≥ 4.5 mg/L
- Executive summary:
The toxicity of the test substance was determined by measuring the bacterial growth inhibition of the test substance to the bacterium Pseudomonas putida. The study was conducted as described by Bringmann and Kühn (1980). The bacterium was exposed to nominal concentrations of 1 mg/L and 4.5 mg/L of the test substance for 6 hours. The optical density of each flask content was measured at 600 nm on a spectrophotometer. It was not possible to test at higher concentrations due to the low water solubility of the test substance. The nominal 1 and 4.5 mg/L solutions of the test substance both gave 0% inhibition of growth. Based on these findings, the EC10 and EC50 values were both determined to be >4.5 mg test material per L (nominal). The NOEC was determined to be ≥ 4.5 mg/L
Reference
Table 1. Optical denisity results
Flask number |
Contents |
Mean optical density at 600nm (4cm cells) |
Minus value for uninoculated solution |
% Inhibition |
1-3 |
Control |
0.553 |
0.509 |
|
4-6 |
18 mg/L 3,5 dichlorophenol |
0.031 |
|
94 |
11,12 |
4.5 mg/L test substance |
0.657 |
0.626 |
0 |
13,14 |
1 mg/L test substance |
0.654 |
0.624 |
0 |
15 |
Blank uninoculated |
0.044 |
|
|
18 |
4.5 mg/L test substance uninoculated |
0.031 |
|
|
19 |
1 mg/L test substance uninoculated |
0.030 |
|
|
Description of key information
6- h EC50 >4.5 mg/L, Pseudomonas putida, Bringmann and Kühn (1980), Tapp 1989
6- h NOEC ≥4.5 mg/L, Pseudomonas putida, Bringmann and Kühn (1980), Tapp 1989
Key value for chemical safety assessment
- EC10 or NOEC for microorganisms:
- 4.5 mg/L
Additional information
The toxicity of the test substance was determined by measuring the bacterial growth inhibition of the test substance to the bacterium Pseudomonas putida. The study was conducted as described by Bringmann and Kühn (1980). The bacterium was exposed to nominal concentrations of 1 mg/L and 4.5 mg/L of the test substance for 6 hours. The optical density of each flask content was measured at 600 nm on a spectrophotometer. It was not possible to test at higher concentrations due to the low water solubility of the test substance. The nominal 1 and 4.5 mg/L solutions of the test substance both gave 0% inhibition of growth. Based on these findings, the EC10 and EC50 values were both determined to be >4.5 mg test material per L (nominal). The NOEC was determined to be ≥ 4.5 mg/L
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