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EC number: 266-257-8 | CAS number: 66215-27-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: short-term
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Study period:
- 1986/01/09 to 1986/01/23
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Justification for type of information:
- Study performed under GLP conditions. The guidelines followed in this study are OECD Guideline No 207 (1984). No deviations occurred from these guidelines
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 986
- Report date:
- 1986
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
- Version / remarks:
- 1984
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- EC Number:
- 266-257-8
- EC Name:
- N-cyclopropyl-1,3,5-triazine-2,4,6-triamine
- Cas Number:
- 66215-27-8
- Molecular formula:
- C6H10N6
- IUPAC Name:
- N2-cyclopropyl-1,3,5-triazine-2,4,6-triamine
Constituent 1
- Specific details on test material used for the study:
- The test material is Cyromazine. It is a white to beige powder.
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Details on preparation and application of test substrate:
- A stock mixture was prepared for each test concentration by premixing the corresponding amount of test substance with 200 g fine quartz sand. The dry stock mixtures were mixed with the dry premixed artificial soil in a HOBART—blender. Then the required amount of water to obtain the specified moisture content was added. After dispensing the blend into the test containers the worms (Eisenia fetida) were transferred into the containers.
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ANIMALS
- Source: External Non-Lab source
- Number of worms (Eisenia fetida): 40 worms per concentration and control, 10 worms per test container
- Age at study initiation: more than 2 months old with clitellum
- Weight: Refer to table 1 in any other information on results incl. tables'
- Feeding: None
- Acclimation period: 24 hours in artificial soil
Study design
- Study type:
- laboratory study
- Substrate type:
- artificial soil
- Remarks:
- The test material was first added to fine quartz sand, mixed and then added to dry premixed artificial soil.
- Total exposure duration:
- 14 d
Test conditions
- Test temperature:
- Maintained at 20 ± 2°C
- pH:
- At test initiation: 5.8 to 6.1
- Moisture:
- At test initiation: 38 to 40%
At test end: 33 to 37% - Details on test conditions:
- Artificial soil used
4 vessels in total for each concentration and control
10 worms (Eisenia fetida) per vessel
40 worms in total tested for each concentration and control
The effect of cyromazine on the survival and condition of the earthworm (Eisenia fetida) was investigated by the addition of 62.5, 125, 250, 500, and 100mg/kg (dry weight of soil) to an artificial soil.
The test material was first added to fine quartz sand, mixed and then added to dry premixed artificial soil. Mixture was then homogenized. Afterwards mixture was hydrated. 750g of the treated soil was added to a 1.5L crystallizing dish with a watch glass cover, this served as the test chamber.
Worms were weighed at start and after 14 days.
Condition of worms was recorded after 7 and 14 days
Worms were maintained under continuous illumination (400 - 600 lux) for entire 14 day test duration - Nominal and measured concentrations:
- Nominal concentrations (mg/kg): 62.5, 125, 250, 500, and 1000
Results and discussion
Effect concentrations
- Key result
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 000 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- act. ingr.
- Basis for effect:
- mortality
- Details on results:
- The pH of the soil at test initiation ranged from 5.8 to 6.1 and the soil moisture content at the end of the 14-day exposure phase was 33-37 % (the initial moisture level was 38-40%) The worms (Eisenia fetida) were maintained at 20 ± 2°C under continuous illumination (400-600 lux) for the entire 14 day duration of the test. The mortality observed in the 125 mg/kg treatment must be treated with caution as no mortalities were observed in either the 250 or 500 mg/kg treatment In the 1000 mg/kg treatment 2 worms (Eisenia fetida) were recorded as being flaccid after 7 days. After 14-days exposure 2, 1, 2, 4 and 9 worms were recorded as flaccid in the 62 5, 125, 250, 500 and 1000 mg/kg treatments respectively.
Any other information on results incl. tables
Table 1
Acute toxicity of test material on the earthworm (Eisenia fetida) in artificial soil
Treatment (mg/kg) | Mortality | Average live weight of worms (g) | % Change in weight of worms over 14 days | ||
7 day | 14 day | 0 day | 14 day | ||
% | % | ||||
Control | 0 | 0 | 420 | 360 | -14 3 |
62 5 | 0 | 0 | 420 | 310 | -26 2 |
125 | 0 | 5 | 410 | 380 | -7 3 |
250 | 0 | 0 | 440 | 420 | -4 5 |
500 | 0 | 0 | 450 | 390 | -13 3 |
1000 | 0 | 2 5 | 440 | 380 | -13 6 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 14-day LC50 for cyromazine with the earthworm (Eisenia fetida) is >1000 mg/kg.
- Executive summary:
The effect of technical cyromazine on the survival and condition of the earthworm (Eisenia fetida) was investigated by the addition of 62 5, 125, 250, 500, and 100mg/kg (dry weight of soil) cyromazine an artificial soil. Study performed under GLP conditions. The guidelines followed in this study are OECD Guideline No 207 (1984). No deviations occurred from these guidelines.
The mortality observed in the 125 mg/kg treatment must be treated with caution as no mortalities were observed in either the 250 or 500 mg/kg treatment In the 1000 mg/kg treatment 2 worms were recorded as being flaccid after 7 days After 14-days exposure 2, 1, 2, 4 and 9 worms were recorded as flaccid in the 62 5, 125, 250, 500 and 1000 mg/kg treatments respectively. The 14-day LC50 for cyromazine with the earthworm (Eisenia fetida) is >1000 mg/kg.
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