[No public or meaningful name is available]

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

04.11.2020 - 16.06.2021

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Qualifier:

according to guideline

Guideline:

other: Commission Regulation (EC) No 440/2008 C.4-C

Qualifier:

according to guideline

Guideline:

other: International Standard ISO 9439

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Name of test substance: Emuldur 3643
Test substance No.: 19/0071-2
Batch identification: 27771924U0
Purity: 100 % UVCB
Storage stability: Expiry date: 15 Jul 2021

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal waste water treatment plant Lambsheim, Germany
- Preparation of inoculum for exposure: Inoculum was pre-aerated two days before the start of the test
- Initial cell/biomass concentration: 30 mg/L dry substance

Duration of test (contact time):

28 d

Initial conc.:

20 mg/L

Based on:

TOC

Initial conc.:

34 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

CO2 evolution

Details on study design:

TEST CONDITIONS
- Test temperature: 22 ± 2 °C

TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: pre-aeration for two days before test start
- Measuring equipment: carbon dioxide trap
- Test performed in open system: no

SAMPLING
- Sampling frequency: every other day

CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Other: Inhibition control

Reference substance:

aniline

Remarks:

20 mg TOC/L

Parameter:

% degradation (CO2 evolution)

Value:

5

St. dev.:

5

Sampling time:

28 d

Details on results:

Emuldur 3643 achieved a maximum degree of biodegradation 5 ±5% CO2/ThCO2
(mean of two replicates ±SD, one day after acidification) after an exposure period of
28 days.
Because the test substance is a UVCB, the criterion of the ten days window was not applied
in the evaluation of results.
The test substance did not reach 10% biodegradation even by the end of incubation, so
there is no adaptation period and 10-day window applicable in this test. A classification of
ready biodegradability is therefore not applicable.
Emuldur 3643 can therefore be evaluated as not readily biodegradable in this carbon
dioxide evolution test, based on the quantitative determination of the formed carbon dioxide
in the test substance assays by comparison with the calculated maximal theoretical carbon
dioxide production.
Based on the rate of biodegradation of 5% CO2/ThCO2 at the end of exposure, the Emuldur
3643 can be further evaluated as poorly biodegradable in this test.

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Emuldur 3643 can therefore be evaluated as not readily biodegradable in this carbon
dioxide evolution test, based on the quantitative determination of the formed carbon dioxide
in the test substance assays by comparison with the calculated maximal theoretical carbon
dioxide production.
Based on the rate of biodegradation of 5% CO2/ThCO2 at the end of exposure, the Emuldur
3643 can be further evaluated as poorly biodegradable in this test.

Description of key information

Not readily biodegradable (according to OECD criteria).

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater