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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2019
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Report date:
2019

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: Essai d’inhibition de la croissance des algues d’eau douce avec des algues vertes unicellulaires (Pseudokirchneriella subcapitata, NF EN ISO 8692, 2012
Principles of method if other than guideline:
The objective of this test is to determine the effects of the product tested on the growth of unicellular green algae after 72 h without change of medium (static test).
Algae of the species Pseudokirchneriella subcapitata, obtained from cultures in the exponential phase of growth, are placed in each of the concentrations of the sample to be tested as well as in the test medium (control).
After 72 h, the cell concentrations are determined in each concentration by microscopy (Malassez cell). The percentages of inhibition of the growth rate are then calculated relative to the average of the control tests.
The test takes place in two stages:
1) Screening test ("range-finding") in order to identify at least the concentrations of product tested which inhibit between 5% and 75% of the rate of algae growth, if possible Then
2) Complete test to determine if possible the CEr50-72h, CEr20-72h, CEr10-72h and the NOEC in mg / L of product.
Duration of the test: 72 h
Renewal of test solutions: none (static mode)
Test medium: ISO medium (composition in appendix 1).
Temperature: 21-25 ° C.
Illuminance: constant, daylight type (400-700 nm), approximately 7500 lux (55 microeinstein / m² / s).
Ventilation: none.
Agitation: constant, around 250 revolutions / min.
Initial cell concentration: approximately 104 cells per mL from a 3-4 day pre-culture.
Containers and test volume: 30 mL vials (20 mL test volume).
GLP compliance:
not specified
Remarks:
this test was not carried out initially to meet the REACH registration requirement, hence the fact that it is not carried out under GLP.

Test material

Constituent 1
Chemical structure
Reference substance name:
Dioctadecyl ether
Cas Number:
6297-03-6
Molecular formula:
C36H74O
IUPAC Name:
Dioctadecyl ether
Constituent 2
Chemical structure
Reference substance name:
Dieicosyl ether
Cas Number:
502696-65-3
Molecular formula:
C40H82O
IUPAC Name:
Dieicosyl ether
Constituent 3
Chemical structure
Reference substance name:
Didocosyl ether
Cas Number:
77456-42-9
Molecular formula:
C44H90O
IUPAC Name:
Didocosyl ether
Constituent 4
Chemical structure
Reference substance name:
Methanesulphonic acid
EC Number:
200-898-6
EC Name:
Methanesulphonic acid
Cas Number:
75-75-2
Molecular formula:
CH4O3S
IUPAC Name:
methanesulphonic acid
Constituent 5
Chemical structure
Reference substance name:
Octadecan-1-ol
EC Number:
204-017-6
EC Name:
Octadecan-1-ol
Cas Number:
112-92-5
Molecular formula:
C18H38O
IUPAC Name:
octadecan-1-ol
Constituent 6
Chemical structure
Reference substance name:
Icosan-1-ol
EC Number:
211-119-4
EC Name:
Icosan-1-ol
Cas Number:
629-96-9
Molecular formula:
C20H42O
IUPAC Name:
icosan-1-ol
Constituent 7
Chemical structure
Reference substance name:
Docosan-1-ol
EC Number:
211-546-6
EC Name:
Docosan-1-ol
Cas Number:
661-19-8
Molecular formula:
C22H46O
IUPAC Name:
docosan-1-ol
Constituent 8
Chemical structure
Reference substance name:
betaine glycine mesylate
Molecular formula:
C6NO5
IUPAC Name:
betaine glycine mesylate
Constituent 9
Chemical structure
Reference substance name:
Octadecyl betainate mesylate
Molecular formula:
C24NO4
IUPAC Name:
Octadecyl betainate mesylate
Constituent 10
Chemical structure
Reference substance name:
Eicosyl betainate mesylate
Molecular formula:
C26NO4
IUPAC Name:
Eicosyl betainate mesylate
Constituent 11
Chemical structure
Reference substance name:
Docosyl betainate mesylate
Cas Number:
Docosyl betainate mesylate
Molecular formula:
C28NO4
IUPAC Name:
Docosyl betainate mesylate

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
SFE: Solubilized fraction or forming a homogeneous suspension in water, obtained from a loading rate in mg / L of the product to be studied.
To prepare each ESF, a quantity of product to be studied is brought into contact with test medium at the load rate tested, in a closed container, with orbital agitation for 24 hours at approximately 110 rpm, at 20 ° +/- 2 ° C.
After shaking, the vials containing the loading levels are subjected to ultrasound for 1 minute. The aqueous fraction of each loading rate (FSE) is then recovered.
The SFE are used immediately for the performance of the test but the results are expressed as loading rate.

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
72 h

Test conditions

Test temperature:
21-25°C.
pH:
8.1 +/- 0.2
Nominal and measured concentrations:
Nominal: 100mg/L, 53mg/L, 31mg/L, 18mg/L, 11mg/L
Reference substance (positive control):
yes

Results and discussion

Effect concentrationsopen allclose all
Key result
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
ca. 31.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC20
Effect conc.:
ca. 15.9 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
EC10
Effect conc.:
ca. 10.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
growth rate
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
ca. 11 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat. (dissolved fraction)
Basis for effect:
growth rate
Results with reference substance (positive control):
Positive control: K2Cr2O7
EC50-72h = 0.99mg/L (to be valid, the value should be between 0.92 mg/L and 1.46mg/L)

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Executive summary:

The toxicity of the test item to aquatic algae was performed according to the French guideline:


"Essai d’inhibition de la croissance des algues d’eau douce avec des algues vertes unicellulaires (Pseudokirchneriella subcapitata, NF EN ISO 8692, 2012"


The results were the following:


EC50 72h=31.3mg/L


EC20 72h=15.9mg/L


EC10 72h=10.8mg/L


NOEC 72h=11mg/L


 


 

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