Ethanol, 2-amino-, reaction products with ammonia, by-products from, distn. Residues

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Feb 1992

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study performed according to guidelines and under GLP. No analytical monitoring of substance.

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

Direct dispersion in de-chlorinated tap water. The substance was known to adsorb to surfaces, so glass vessels were first saturated with substance, by soaking overnight with solution.

Test organisms (species):

Daphnia magna

Details on test organisms:

Laboratory culture originating from a strain supplied by the Institut National de Recherche Chimique Appliquee, {I.R.CH.A}, France.
Culture: At 21°C in polypropylene vessels containing two litres of dechlorinated and aged tap water. Cultures were fed daily with a suspension of mixed algae {predominantly Chlorella spp.}. Culture conditions ensure that reproduction is by parthenogenesis.
Gravid adults were isolated 24 hours prior to initiation of the test. Young daphnids produced overnight were used for testing.

Test type:

static

Water media type:

freshwater

Total exposure duration:

48 h

Hardness:

50 mg/L as CaCO3

Test temperature:

22 °C

pH:

7.9 - 9.7

Dissolved oxygen:

7.9 - 8.6 mg/L

Nominal and measured concentrations:

3.2, 5.6, 10, 18, 32, 56, 100, 180 and 320 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass jars each containing 200 ml test solution and covered to reduce evaporation
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
-
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: De-chlorinated tap-water

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16h light 8 h dark

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

64 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality

Remarks on result:

other: 95% CL 56 - 75 mg/L

Conclusions:

The substance can be considered harmful to Daphnia Magna.

Executive summary:

In a Acute toxicity test according to OECD 202 with Daphnia Magna, a 48 h EC50 of 64 mg/L was observed.

Description of key information

The substance can be considered harmful to aquatic invertebrates.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

64 mg/L

Additional information

In a 48 h acute toxicity study with the substance a EC50 value of 64 mg/L was obtained. For diethanolamine which is a large component of the multiconstituent substance a similar value is available.