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Diss Factsheets
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EC number: - | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 19.11.2010
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Report date:
- 2010
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- Accordint to OECD 202 resp. EU C.2
- GLP compliance:
- yes (incl. QA statement)
Test material
Reference
- Name:
- Unnamed
- Type:
- Constituent
- Specific details on test material used for the study:
- The water-accommodated fraction was prepared by weighing of the nominal load, adding the corresponding amount of dilution water and shaking vigorously for 24 hours. The resulting solution was filtrated through 0.45 µm filters
Sampling and analysis
- Analytical monitoring:
- not specified
Test solutions
- Details on test solutions:
- Sample Preparation: 25 ml filtrated solution was filled up to 100 ml. After addition of 1 ml 2m NaOH and 7 g NaCl, the solution was extracted twice with iso-hexane (4 ml and 3 ml). The organic phase was dried with Na2SO4 and transferred in a 10 ml volumetric flask. Then 1 ml ISTD Diphenylamine (1000 mg/l in iso-hexane) was added and the volumetric flask was filled up with iso-Hexane.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species: Daphnia magna
Variety: STRAUS
Strain: Berlin
Sex: female
Age: between 0 and 24 hours
Origin: Umweltbundesamt Berlin
Arrival of Strain: 27. Sep. 2007
Selection of the test system was made following the proposal af the guidelines.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- The test vessels were left to stand for 48 hours. After 24 and 48 hours, the immobilised daphnia were counted. Daphnia are considered immobilised if they perform no movements or are only able to move their antennae when the beaker is gently agitated.
Test conditions
- Test temperature:
- 19.7 - 20.6 °C
- pH:
- 7.7 - 8.0
- Dissolved oxygen:
- The concentration of dissolved oxygen stayed above 7.9 mg/L throughout the test.
- Nominal and measured concentrations:
- Nominal Conc. 100 mg/L
Measured Conc. 0h: 99.6 mg/L
Measured Conc 48h: 93.2 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate K2Cr2O7 was used as positive control. the 24h-EC50i of potassium dichromate was determined as 1.7 mg/L, lying within the demanded range of 0.6 - 1.7 mg/L.
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- The main study was performed using one concentration containing 100 mg/L. Twenty daphnia were exposed to the test item for 48 hours in a static test system. After 24 and 48 hours, the immobilised daphnia were counted.
The concentration showed no toxicity. None of the animals were immobilised in the control.
The 24h-EC50i of potassium dichromate was tested in a current positive control test. The value was determined as 1.7 mg/L, lying within the demanded range of 0.6 - 1.7 mg/L.
At the beginning and at the end of the test, the content of the test item in the test solutions was determined by measurement of Dicyclohexylamine after saponification via GC. The recovery after 48 hours was 94% of the start concentration, the correlation between nominal and measured concentration was very good. Therefore, the determination of the biological results was based on the nominal concentration.
The following results were determined for the test item Dicyclohexylammonium Isostearat (species: Daphnia magna).
24h-NOEC = 100 mg/L
48h-NOEC = 100 mg/L
24h-EC50i > 100 mg/L
48h-EC50i > 100 mg/L - Executive summary:
The following results were determined for the test item Dicyclohexylammonium Isostearat (species: Daphnia magna).
24h-NOEC = 100 mg/L
48h-NOEC = 100 mg/L
24h-EC50i > 100 mg/L
48h-EC50i > 100 mg/L
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