3-tert-butyl-1-(3,5-dicarboxyphenyl)-1H-pyrazol-5-aminium chloride

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11th July 2019 to 8th August 2019

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2019

Report date:

2019

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 D (Ready Biodegradability: Closed Bottle Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

3-tert-butyl-1-(3,5-dicarboxyphenyl)-1H-pyrazol-5-aminium chloride

EC Number:

941-661-3

Molecular formula:

C15H18ClN3O4

IUPAC Name:

3-tert-butyl-1-(3,5-dicarboxyphenyl)-1H-pyrazol-5-aminium chloride

impurity 1

Reference substance name:

Unidentified impurity

IUPAC Name:

Unidentified impurity

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

Secondary effluent, microorganisms from a domestic waste water treatment plant.
The secondary effluent was supplied from the sewage plant for domestic sewage in Veszprém, Hungary.
The secondary effluent used for this study was allowed to settle approximately for an hour, then the decanted effluent was aerated until use (not later than an hour).

Duration of test (contact time):

28 d

Initial test substance concentration

Initial conc.:

ca. 3 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

O2 consumption

Reference substance

Reference substance:

benzoic acid, sodium salt

Results and discussion

% Degradation

Key result

Parameter:

% degradation (O2 consumption)

Value:

23.6

Sampling time:

28 d

Details on results:

Under the test conditions the percentage biodegradation of S194997 reached a mean of 23.6 % after 28 days based on the ThOD of the test item.

BOD5 / COD results

Results with reference substance:

The reference item Sodium benzoate was sufficiently degraded to a mean of 79.2 % after 14 days, and to a mean of 81.7 % after 28 days of incubation, based on ThOD.
In the toxicity control containing both, the test item and the reference item Sodium benzoate, a mean of 45.3 % biodegradation was noted within 14 days and 44.1% biodegradation after 28 days of incubation.
According to the test guidelines the test item can be assumed to be not inhibitory at the applied concentration level of 3.0 mg/L on the secondary effluent microorganisms because degradation was >25 % within 14 days

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Based on the results of this study, the test item is considered not readily biodegradable.