1,2-Naphtoquinonediazide-5-sulfonylchloride (or sulfonic acid) esters with 4,6-bis(1-(4-hydroxyphenyl)-1-methylethyl)-1,3-dihydroxybenzene

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

June 9, 2016 - June 13, 2016

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

no analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Principles of method if other than guideline:

- Principle of test: mortality test to determline acute toxicity
- Parameters analysed / observed: mortality

GLP compliance:

not specified

Remarks:

Study conducted in Japan, not for REACH purposes

Test material

Test material information

Constituent 1

Reference substance name:

4-[2-(2,4-dihydroxy-5-{2-[4-({[6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl]propan-2-yl}phenyl)propan-2-yl]phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate

Molecular formula:

C44H34N4O10S2

IUPAC Name:

4-[2-(2,4-dihydroxy-5-{2-[4-({[6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl]propan-2-yl}phenyl)propan-2-yl]phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate

Constituent 2

Reference substance name:

4-(2-{2,4-dihydroxy-5-[2-(4-hydroxyphenyl)propan-2-yl]phenyl}propan-2-yl)phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate

Molecular formula:

C34H30N2O7S

IUPAC Name:

4-(2-{2,4-dihydroxy-5-[2-(4-hydroxyphenyl)propan-2-yl]phenyl}propan-2-yl)phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item and dilution water were mixed to prepare each nominal concentration and stirred for 48 h under shading. Then the suspension was filtered with a glass fiber (GB-140, 0.4 µm pore size, Toyo Roshi) by suction to prepare the test solution. The test item was treated under yellow fluorescent light.
- Evidence of undissolved material: Appearance of test solution: colorless without insoluble matter (at the start of the exposure: visual)

Test organisms

Test organisms (species):

Oryzias latipes

Details on test organisms:

TEST ORGANISM

- Length at study initiation: 2.5-3.0 cm
- Weight at study initiation: 0.15 - 0.29 g

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

24 ± 1 °C

pH:

7.7-8.0

Dissolved oxygen:

7.1-8.3

Nominal and measured concentrations:

nominal: 1, 10, 100 mg/l and control

Details on test conditions:

TEST SYSTEM
- Test vessel: Glass tank
- No. of organisms per concentration: 7

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality

TEST CONCENTRATIONS
- Test concentrations: 1, 10, 100 mg/l

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No mortality observed

Any other information on results incl. tables

Sublethal observations / clinical signs:

Test Level (mg/L)	Cumulative mortality (%)
	24h	48h	72h	96 h
1	0	0	0	0
10	0	0	0	0
100	0	0	0	0
Control	0	0	0	0