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Diss Factsheets

Administrative data

Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
June 9, 2016 - June 13, 2016
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
no analytical monitoring

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Principles of method if other than guideline:
- Principle of test: mortality test to determline acute toxicity
- Parameters analysed / observed: mortality
GLP compliance:
not specified
Remarks:
Study conducted in Japan, not for REACH purposes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-[2-(2,4-dihydroxy-5-{2-[4-({[6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl]propan-2-yl}phenyl)propan-2-yl]phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate
Molecular formula:
C44H34N4O10S2
IUPAC Name:
4-[2-(2,4-dihydroxy-5-{2-[4-({[6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl]propan-2-yl}phenyl)propan-2-yl]phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate
Constituent 2
Chemical structure
Reference substance name:
4-(2-{2,4-dihydroxy-5-[2-(4-hydroxyphenyl)propan-2-yl]phenyl}propan-2-yl)phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate
Molecular formula:
C34H30N2O7S
IUPAC Name:
4-(2-{2,4-dihydroxy-5-[2-(4-hydroxyphenyl)propan-2-yl]phenyl}propan-2-yl)phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item and dilution water were mixed to prepare each nominal concentration and stirred for 48 h under shading. Then the suspension was filtered with a glass fiber (GB-140, 0.4 µm pore size, Toyo Roshi) by suction to prepare the test solution. The test item was treated under yellow fluorescent light.
- Evidence of undissolved material: Appearance of test solution: colorless without insoluble matter (at the start of the exposure: visual)

Test organisms

Test organisms (species):
Oryzias latipes
Details on test organisms:
TEST ORGANISM

- Length at study initiation: 2.5-3.0 cm
- Weight at study initiation: 0.15 - 0.29 g

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
24 ± 1 °C
pH:
7.7-8.0
Dissolved oxygen:
7.1-8.3
Nominal and measured concentrations:
nominal: 1, 10, 100 mg/l and control
Details on test conditions:
TEST SYSTEM
- Test vessel: Glass tank
- No. of organisms per concentration: 7

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality

TEST CONCENTRATIONS
- Test concentrations: 1, 10, 100 mg/l
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
No mortality observed

Any other information on results incl. tables

Sublethal observations / clinical signs:

 Test Level (mg/L)              Cumulative mortality (%)
 24h 48h  72h  96 h 
 1 0
 10
 100
 Control 0 0 0 0

Applicant's summary and conclusion