Registration Dossier
Registration Dossier
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EC number: 606-834-7 | CAS number: 21806-61-1
Hydrolysis
Administrative data
Link to relevant study record(s)
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 111 (Hydrolysis as a Function of pH)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
Ambient(21 to 29℃)
- Solubility and stability of the test material in the solvent/vehicle and the exposure medium:
soluble and stable
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
- Treatment of test material prior to testing (e.g. warming, grinding):
no
- Preliminary purification step (if any):
- Final concentration of a dissolved solid, stock liquid or gel:
not specified - Radiolabelling:
- no
- Analytical monitoring:
- yes
- Remarks:
- HPLC
- Details on sampling:
- - Sampling intervals for the parent/transformation products:
- Sampling method:
A duplicate sample of each pH buffers were taken immediately after the addition of the test item on 0 hour (pH 4.0, pH 7.0 and pH 9.0) and incubated at 15± 0.5ºC, 25 ± 0.5ºC and 50 ± 0.5ºC.
All the test samples were analyzed for active content concentration at each occasion as follows.
For 50ºC: 0th hour, 4th hour, 8th hour, 12th hour, 20th hour, 24th hour, 30th hour,48th hour (day 2), 72nd hour (day 3) for all three pH buffers.
For 25ºC: 0th hour, 4th hour, 8th hour, 12th hour, 20th hour, 24th hour, 30th hour, 48th hour (day 2), 72nd hour (day3), 144th hour (day 6), 192nd hour (day 8), 264th hour (day 11), 360th hour (day 15) for all three pH buffers.
For 15ºC: 0th hour, 4th hour, 8th hour, 12th hour, 20th hour, 24th hour (day 1), 30th hour, 48th hour (day 2), 72nd hour (day 3), 144th hour (day 6), 360th hour (day 15), 528th hour (day 22), 720th hour (day 30) for all three pH buffers. - Buffers:
- - Type and final molarity of buffer:
- Composition of buffer:
Buffer Solution of pH 4: An aliquot of 12.0 mL of 0.1 N sodium hydroxide solution and 1500.0 mL of 0.1 M potassium biphthalate solution was diluted to 3000 mL with Milli-Q water. The pH of the resulting buffer solution was found to be 3.96 and then pH of buffer solution was adjusted to 4.00 using 0.1 N sodium hydroxide solution.
Buffer Solution of pH 7: An aliquot of 889.90 mL of 0.1 N sodium hydroxide solution and 1500.0 mL of 0.1 M potassium phosphate solution was diluted to 3000 mL with Milli-Q water. The pH of the resulting buffer solution was found to be 6.97 and then pH of buffer solution was adjusted to 7.00 using 0.1 N sodium hydroxide solution.
Buffer Solution of pH 9: An aliquot of 639.0 mL of 0.1 N sodium hydroxide solution and 1500.0 mL of 0.1 M boric acid solution was diluted to 3000 mL with Milli-Q water. The pH of the resulting buffer solution was found to be 8.95 and then pH of buffer solution was adjusted to 9.00 using 0.1 N sodium hydroxide solution. - Details on test conditions:
- TEST SYSTEM
- Type, material and volume of test flasks, other equipment used:
50ml amber coloured glass bottle
- Sterilisation method:
The glassware (before use) and prepared buffer solutions were sterilized by autoclaving at 121.0°C for about 15 minutes and filtered passing through 0.2 µm sterilized filter and oxygen in the solutions was excluded by sonication for five minutes.
- Light:
dark
TEST MEDIUM
- Volume used/treatment
10 ml
- Kind and purity of water:
Milli-Q water
- Preparation of test medium:
The prepared test item solutions in each pH 4.0, 7.0 and 9.0 buffers was maintained at temperature of 50 ± 0.5°C for a period of 5 days duration of the study and it was continuously monitored and recorded during preliminary test in all working days (once in a day) throughout the course of the study.
- Renewal of test solution:
no - Duration:
- 30 d
- pH:
- 4
- Temp.:
- 15 °C
- Initial conc. measured:
- 9.37 mg/L
- Duration:
- 15 d
- pH:
- 4
- Temp.:
- 25 °C
- Initial conc. measured:
- 9.37 mg/L
- Duration:
- 3 d
- pH:
- 4
- Temp.:
- 50 °C
- Initial conc. measured:
- 9.37 mg/L
- Duration:
- 30 d
- pH:
- 7
- Temp.:
- 15 °C
- Initial conc. measured:
- 9.3 mg/L
- Duration:
- 15 d
- pH:
- 7
- Temp.:
- 25 °C
- Initial conc. measured:
- 9.3 mg/L
- Duration:
- 3 d
- pH:
- 7
- Temp.:
- 50 °C
- Initial conc. measured:
- 9.3 mg/L
- Duration:
- 30 d
- pH:
- 9
- Temp.:
- 15 °C
- Initial conc. measured:
- 9.38 mg/L
- Duration:
- 15 d
- pH:
- 9
- Temp.:
- 25 °C
- Initial conc. measured:
- 9.38 mg/L
- Duration:
- 3 d
- pH:
- 9
- Temp.:
- 50 °C
- Initial conc. measured:
- 9.38 mg/L
- Number of replicates:
- duplicate for each occasion
- Positive controls:
- no
- Negative controls:
- no
- Preliminary study:
- The rate of hydrolysis at 50 ± 0.5ºC was assessed for pH 4, pH 7 and pH 9. The percent degradation of the test item were found to be100% at each pH 4, pH 7 and pH 9 buffers over a period of 5 days of incubation. Hence, the test item was hydrolytically unstable in pH 4, pH 7 and pH 9 at 50 ± 0.5ºC after 5 days.
Based on the preliminary test results, the test item 1,3-Propene Sultone was hydrolytically unstable in pH 4, pH 7 and pH 9 at 50°C. - Transformation products:
- no
- Remarks:
- From the Tier 2 results, the HPLC chromatograms of the test item in 3 pH buffers solutions incubated at 3 temperatures evidenced that there were no peaks identified other than the test item and diluent peaks. There is no formation of hydrolyzed product.
- pH:
- 4
- Temp.:
- 15 °C
- Hydrolysis rate constant:
- 0.002 h-1
- DT50:
- 288.75 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 4
- Temp.:
- 25 °C
- Hydrolysis rate constant:
- 0.007 h-1
- DT50:
- 97.61 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 4
- Temp.:
- 50 °C
- Hydrolysis rate constant:
- 0.07 h-1
- DT50:
- 9.96 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 7
- Temp.:
- 15 °C
- Hydrolysis rate constant:
- 0.003 h-1
- DT50:
- 266.54 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 7
- Temp.:
- 25 °C
- Hydrolysis rate constant:
- 0.007 h-1
- DT50:
- 92.4 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 7
- Temp.:
- 50 °C
- Hydrolysis rate constant:
- 0.007 h-1
- DT50:
- 9.9 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 9
- Temp.:
- 15 °C
- Hydrolysis rate constant:
- 0.002 h-1
- DT50:
- 301.3 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 9
- Temp.:
- 25 °C
- Hydrolysis rate constant:
- 0.007 h-1
- DT50:
- 103.43 h
- Type:
- (pseudo-)first order (= half-life)
- pH:
- 9
- Temp.:
- 50 °C
- Hydrolysis rate constant:
- 0.071 h-1
- DT50:
- 9.71 h
- Type:
- (pseudo-)first order (= half-life)
- Details on results:
- TEST CONDITIONS
- pH, sterility, temperature, and other experimental conditions maintained throughout the study: Yes
MAJOR TRANSFORMATION PRODUCTS
No transformation products
MINOR TRANSFORMATION PRODUCTS
No transformation products
INDICATION OF UNSTABLE TRANSFORMATION PRODUCTS:
No transformation products
VOLATILIZATION (at end of study)
No volatilization
UNIDENTIFIED RADIOACTIVITY (at end of study)
No radioactivity identified - Validity criteria fulfilled:
- yes
- Conclusions:
- PST is hydrolysed, but no hydrolysis products are observed using HPLC method.
The preliminary test at 50± 0.5°C after 5 days, the test item 1,3-Propene Sultone was found to be hydrolytically unstable due to 100% hydrolyzed in pH 4.0, pH 7.0 and pH 9.0 buffer test solutions.
In further tests tier 2, the hydrolysis of the test item was investigated as a function of pH (4, 7 and 9) at 15± 0.5°C, 25± 0.5°C and 50± 0.5°C respectively.
At pH 4, the rate constant and half-life of the reactions at 25± 0.5°C was determined to be 0.0071 h-1 and 97.61 h, respectively.
At pH 7, the rate constant and half-life of the reactions at 25± 0.5°C was determined to be 0.0075 h-1 and 92.40 h, respectively.
At pH 9, the rate constant and half-life of the reactions at 25± 0.5°C was determined to be 0.0067 h-1 and 103.43 h, respectively. - Executive summary:
The hydrolysis of the test item as a function of pH in aqueous solution was determined according to OECD guideline 111.
A preliminary test (Tier 1) was performed. The rate of hydrolysis at 50 ± 0.5°C was assessed for pH 4, 7 and 9.The percent degradation of the test item were 100% at each pH 4, pH 7 and pH 9 buffers over a period of 5 days of incubation. Hence the test item was hydrolytically unstable in pH 4, pH 7 and pH 9 at 50± 0.5°Cafter 5 days.
Based on the results from the Preliminary test / Tier 1, further testing Tier 2 was conducted at pH 4, pH 7 and pH 9with solutions being maintained at 15.0 ± 0.5°C, 25.0 ± 0.5°C and 50.0 ± 0.5°C for various time periods.
Based on tier 2 results, the determined degradation rate constant and half-life values were provided below.
Sterile Buffer
Temperature (°C)
Rate constant kobs(hour-1)
Half Life t1/2(hours)
pH 4
15.0
0.0024
288.75
25.0
0.0071
97.61
50.0
0.0696
9.96
pH 7
15.0
0.0026
266.54
25.0
0.0075
92.40
50.0
0.0700
9.90
pH 9
15.0
0.0023
301.30
25.0
0.0067
103.43
50.0
0.0714
9.71
Based on Tier 2 results, no other breakdown products are detected, hence tier 3 test for the identification of other products was not performed.
The mean logarithms of the relative concentrations were plotted against time using linear regression analysis. At all temperatures of15± 0.5°C, 25± 0.5°C and 50± 0.5°C, linear relationships were obtained and the correlation coefficient (r2) were ranged between 0.9244 to 0.9974.
Conclusion:
The preliminarytest at 50± 0.5°Cafter 5 days, the test item 1,3-Propene Sultone was found to be hydrolytically unstable due to 100% hydrolyzed in pH 4.0, pH 7.0 and pH 9.0 buffer test solutions.
In further tests tier 2, the hydrolysis of the test item was investigated as a function of pH (4, 7 and 9) at 15± 0.5°C, 25± 0.5°C and 50± 0.5°C respectively.
AtpH 4, the rate constant and half-life of the reactions at 25± 0.5°Cwas determined to be 0.0071 h-1and 97.61 h, respectively.
At pH 7, the rate constant and half-life of the reactions at 25± 0.5°Cwas determined to be 0.0075 h-1and 92.40 h, respectively.
At pH 9, the rate constant and half-life of the reactions at 25± 0.5°Cwas determined to be 0.0067 h-1and 103.43 h, respectively.
Reference
TABLE 1. SPECIFICITY
Sample Code |
LOQ Area (mAU) |
Sample Area (mAU) |
% Interference |
||||
Diluent 1 |
69319 |
6561 |
9.47 |
||||
Diluent pH 4 |
69319 |
763 |
1.10 |
||||
Diluent pH 7 |
69319 |
1185 |
1.71 |
||||
Diluent pH 9 |
69319 |
526 |
0.76 |
Interference (%) |
= |
Sample Area × 100 |
|||
LOQArea |
TABLE 2. LIMIT OF DETECTION (LOD) AND LIMIT OF QUANTIFICATION (LOQ)
Sample Code |
LDQSample Conc. (mg/L) |
Sample Peak Area(mAU) |
Diluent Peak Area(mAU) |
Signal to Noise Ratio |
Identification |
||||||
LDQ 1 |
19.9200 |
1452600 |
6561 |
221.40 |
- |
||||||
LDQ 2 |
9.9600 |
719169 |
109.61 |
- |
|||||||
LDQ 3 |
4.9800 |
360266 |
54.91 |
- |
|||||||
LDQ 4 |
1.9920 |
161513 |
24.62 |
- |
|||||||
LDQ 5 |
0.9960 |
69319 |
10.57 |
LOQ |
|||||||
LDQ 6 |
0.4980 |
35021 |
5.34 |
- |
|||||||
LDQ 7 |
0.1992 |
22903 |
3.49 |
LOD |
|||||||
LDQ 8 |
0.0996 |
17689 |
2.70 |
- |
|||||||
LDQ 9 |
0.0498 |
2818 |
0.43 |
- |
Diluent :Acetonitrile
Signal to Noise Ratio |
= |
Sample Peak Area |
|||
Diluent Peak Area |
TABLE 3. LINEARITY CALCULATIONS(DATE OF ANALYSIS: 13/07/2020)
Concentration (mg/L) |
Peak Area (mAU) |
||
0.5015 |
31034 |
||
1.0030 |
68435 |
||
2.5075 |
183408 |
||
5.0150 |
347766 |
||
10.0300 |
693261 |
||
12.5375 |
874745 |
||
Intercept |
257.79 |
||
Slope |
69540.66 |
||
Correlation Coefficient (r) |
0.9999 |
TABLE 4. LINEARITY CALCULATIONS (DATE OF ANALYSIS: 14/07/2020)
Concentration (mg/L) |
Peak Area (mAU) |
||
0.5005 |
27476 |
||
1.0010 |
68907 |
||
2.5025 |
165564 |
||
5.0050 |
347909 |
||
10.0100 |
699716 |
||
12.5125 |
875217 |
||
Intercept |
-6222.98 |
||
Slope |
70473.24 |
||
Correlation Coefficient (r) |
1.0000 |
TABLE 5. LINEARITY CALCULATIONS (DATE OF ANALYSIS: 08/12/2020)
Standard Concentration (mg/L) |
Peak Area (mAU) |
||
0.5050 |
21914 |
||
1.0100 |
69299 |
||
2.5250 |
152620 |
||
5.0500 |
291470 |
||
10.1000 |
559332 |
||
12.6250 |
680070 |
||
Intercept |
10841.62 |
||
Slope |
53737.40 |
||
Correlation Coefficient (r) |
0.9993 |
TABLE 6. DETERMINATION OFACCURACY AND PRECISION OF TEST ITEM INpH 4.0 BUFFER SOLUTIONS
Sample Code |
Peak Area (mAU) |
Slope |
Intercept |
Dilution Factor |
NominalConc. (mg/L) |
Obtained Conc. (mg/L) |
Recovery (%) |
Mean (%) Recovery |
Mean Conc. (mg/L) |
SD (±) |
RSD (%) |
||||||||||||
Control 1 pH 4 |
BLOQ |
69540.66 |
257.79 |
1 |
- |
- |
- |
- |
- |
- |
- |
||||||||||||
Accuracy 1 R1 pH 4 |
71798 |
69540.66 |
257.79 |
1 |
0.9974 |
1.0288 |
103.15 |
104.57 |
1.0430 |
2.83 |
2.71 |
||||||||||||
Accuracy 1 R2 pH 4 |
71810 |
69540.66 |
257.79 |
1 |
0.9974 |
1.0289 |
103.16 |
||||||||||||||||
Accuracy 1 R3 pH 4 |
70626 |
69540.66 |
257.79 |
1 |
0.9974 |
1.0119 |
101.45 |
||||||||||||||||
Accuracy 1 R4 pH 4 |
74446 |
69540.66 |
257.79 |
1 |
0.9974 |
1.0668 |
106.96 |
||||||||||||||||
Accuracy 1 R5 pH 4 |
75255 |
69540.66 |
257.79 |
1 |
0.9974 |
1.0785 |
108.13 |
||||||||||||||||
Control 2 pH 4 |
BLOQ |
69540.66 |
257.79 |
1 |
- |
- |
- |
- |
- |
- |
- |
||||||||||||
Accuracy 2 R1 pH 4 |
706695 |
69540.66 |
257.79 |
1 |
9.9740 |
10.1586 |
101.85 |
100.86 |
10.0594 |
0.71 |
0.70 |
||||||||||||
Accuracy 2 R2 pH 4 |
700150 |
69540.66 |
257.79 |
1 |
9.9740 |
10.0645 |
100.91 |
||||||||||||||||
Accuracy 2 R3 pH 4 |
697208 |
69540.66 |
257.79 |
1 |
9.9740 |
10.0222 |
100.48 |
||||||||||||||||
Accuracy 2 R4 pH 4 |
701491 |
69540.66 |
257.79 |
1 |
9.9740 |
10.0838 |
101.10 |
||||||||||||||||
Accuracy 2 R5 pH 4 |
693418 |
69540.66 |
257.79 |
1 |
9.9740 |
9.9677 |
99.94 |
TABLE 7. DETERMINATION OFACCURACY AND PRECISION OF TEST ITEM INpH 7.0 BUFFER SOLUTIONS
Sample Code |
Peak Area (mAU) |
Slope |
Intercept |
Dilution Factor |
NominalConc. (mg/L) |
Obtained Conc. (mg/L) |
Recovery (%) |
Mean (%) Recovery |
Mean Conc. (mg/L) |
SD (±) |
RSD (%) |
Control 1 pH 7 |
BLOQ |
69540.66 |
257.79 |
1 |
- |
- |
- |
- |
- |
- |
- |
Accuracy 1 R1 pH 7 |
74997 |
69540.66 |
257.79 |
1 |
1.0034 |
1.0748 |
107.12 |
104.02 |
1.0438 |
4.14 |
3.98 |
Accuracy 1 R2 pH 7 |
76718 |
69540.66 |
257.79 |
1 |
1.0034 |
1.0995 |
109.58 |
||||
Accuracy 1 R3 pH 7 |
69824 |
69540.66 |
257.79 |
1 |
1.0034 |
1.0004 |
99.70 |
||||
Accuracy 1 R4 pH 7 |
71329 |
69540.66 |
257.79 |
1 |
1.0034 |
1.0220 |
101.85 |
||||
Accuracy 1 R5 pH 7 |
71339 |
69540.66 |
257.79 |
1 |
1.0034 |
1.0222 |
101.87 |
||||
Control 2 pH 7 |
BLOQ |
69540.66 |
257.79 |
1 |
- |
- |
- |
- |
- |
- |
|
Accuracy 2 R1 pH 7 |
687693 |
69540.66 |
257.79 |
1 |
10.0340 |
9.8854 |
98.52 |
98.90 |
9.9239 |
0.51 |
0.52 |
Accuracy 2 R2 pH 7 |
688958 |
69540.66 |
257.79 |
1 |
10.0340 |
9.9036 |
98.70 |
||||
Accuracy 2 R3 pH 7 |
687594 |
69540.66 |
257.79 |
1 |
10.0340 |
9.8839 |
98.50 |
||||
Accuracy 2 R4 pH 7 |
691446 |
69540.66 |
257.79 |
1 |
10.0340 |
9.9393 |
99.06 |
||||
Accuracy 2 R5 pH 7 |
696172 |
69540.66 |
257.79 |
1 |
10.0340 |
10.0073 |
99.73 |
TABLE 8. DETERMINATION OFACCURACY AND PRECISIONOF TEST ITEM INpH 9.0 BUFFER SOLUTIONS
Sample Code |
Peak Area (mAU) |
Slope |
Intercept |
Dilution Factor |
NominalConc. (mg/L) |
Obtained Conc. (mg/L) |
Recovery (%) |
Mean (%) Recovery |
Mean Conc. (mg/L) |
SD (±) |
RSD (%) |
Control 1 pH 9 |
BLOQ |
70473.24 |
-6222.98 |
1 |
- |
- |
- |
- |
- |
- |
- |
Accuracy 1 R1 pH 9 |
67984 |
70473.24 |
-6222.98 |
1 |
1.0038 |
1.0530 |
104.90 |
97.63 |
0.9800 |
5.82 |
5.96 |
Accuracy 1 R2 pH 9 |
57584 |
70473.24 |
-6222.98 |
1 |
1.0038 |
0.9054 |
90.20 |
||||
Accuracy 1 R3 pH 9 |
60163 |
70473.24 |
-6222.98 |
1 |
1.0038 |
0.9420 |
93.84 |
||||
Accuracy 1 R4 pH 9 |
65380 |
70473.24 |
-6222.98 |
1 |
1.0038 |
1.0160 |
101.22 |
||||
Accuracy 1 R5 pH 9 |
63093 |
70473.24 |
-6222.98 |
1 |
1.0038 |
0.9836 |
97.99 |
||||
Control 2 pH 9 |
BLOQ |
70473.24 |
-6222.98 |
1 |
- |
- |
- |
- |
- |
- |
- |
Accuracy 2 R1 pH 9 |
694703 |
70473.24 |
-6222.98 |
1 |
10.0380 |
9.9460 |
99.08 |
97.86 |
9.8234 |
2.24 |
2.29 |
Accuracy 2 R2 pH 9 |
698757 |
70473.24 |
-6222.98 |
1 |
10.0380 |
10.0035 |
99.66 |
||||
Accuracy 2 R3 pH 9 |
688214 |
70473.24 |
-6222.98 |
1 |
10.0380 |
9.8539 |
98.17 |
||||
Accuracy 2 R4 pH 9 |
689955 |
70473.24 |
-6222.98 |
1 |
10.0380 |
9.8786 |
98.41 |
||||
Accuracy 2 R5 pH 9 |
658694 |
70473.24 |
-6222.98 |
1 |
10.0380 |
9.4350 |
93.99 |
TABLE 1. CONCENTRATION OF1, 3-PROPENE SULTONEIN pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS ON DAY 0 AT 50°C-TIER 1 TEST
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 |
BLOQ |
346046 |
- |
- |
- |
|
Sample pH 4 R1 Rep 1 |
361514 |
5.0050 |
2.0 |
10.46 |
10.43 |
|
Sample pH 4 R1 Rep 2 |
358344 |
5.0050 |
2.0 |
10.37 |
||
Sample pH 4 R2 Rep 1 |
359750 |
5.0050 |
2.0 |
10.41 |
||
Sample pH 4 R2 Rep 2 |
361685 |
5.0050 |
2.0 |
10.46 |
||
Control pH 7 |
BLOQ |
- |
- |
- |
|
|
Sample pH 7 R1 Rep 1 |
347613 |
5.0050 |
2.0 |
10.06 |
10.11 |
|
Sample pH 7 R1 Rep 2 |
349003 |
5.0050 |
2.0 |
10.10 |
||
Sample pH 7 R2 Rep 1 |
349715 |
5.0050 |
2.0 |
10.12 |
||
Sample pH 7 R2 Rep 2 |
350922 |
5.0050 |
2.0 |
10.15 |
||
Control pH 9 |
BLOQ |
- |
- |
- |
|
|
Sample pH 9 R1 Rep 1 |
350273 |
5.0050 |
2.0 |
10.13 |
10.05 |
|
Sample pH 9 R1 Rep 2 |
346213 |
5.0050 |
2.0 |
10.01 |
||
Sample pH 9 R2 Rep 1 |
344617 |
5.0050 |
2.0 |
9.97 |
||
Sample pH 9 R2 Rep 2 |
348389 |
5.0050 |
2.0 |
10.08 |
TABLE 2. CONCENTRATION OF1, 3-PROPENE SULTONEIN pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS ON DAY 5 AT 50°C-TIER 1 TEST
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 |
BLOQ |
317843 |
- |
- |
- |
- |
Sample pH 4 R1 Rep 1 |
BLOQ |
5.0100 |
2.0 |
BLOQ |
BLOQ |
|
Sample pH 4 R1 Rep 2 |
BLOQ |
5.0100 |
2.0 |
BLOQ |
||
Sample pH 4 R2 Rep 1 |
BLOQ |
5.0100 |
2.0 |
BLOQ |
||
Sample pH 4 R2 Rep 2 |
BLOQ |
5.0100 |
2.0 |
BLOQ |
||
Control pH 7 |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 Rep 1 |
BLOQ |
5.0100 |
2.0 |
BLOQ |
BLOQ |
|
Sample pH 7 R1 Rep 2 |
BLOQ |
5.0100 |
2.0 |
BLOQ |
||
Sample pH 7 R2 Rep 1 |
BLOQ |
5.0100 |
2.0 |
BLOQ |
||
Sample pH 7 R2 Rep 2 |
BLOQ |
5.0100 |
2.0 |
BLOQ |
||
Control pH 9 |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 Rep 1 |
BLOQ |
5.0100 |
2.0 |
BLOQ |
BLOQ |
|
Sample pH 9 R1 Rep 2 |
BLOQ |
5.0100 |
2.0 |
BLOQ |
||
Sample pH 9 R2 Rep 1 |
BLOQ |
5.0100 |
2.0 |
BLOQ |
||
Sample pH 9 R2 Rep 2 |
BLOQ |
5.0100 |
2.0 |
BLOQ |
BLOQ: Below Limit of quantification
TABLE 3. PERCENTAGE DEGRADATION OF TEST ITEM CONCENTRATION AFTER 5 DAYS FROM INITIAL ANALYSIS-TIER 1
Sample Code |
On Day 0, Mean 1, 3-Propene Sultone Concentration (mg/L) |
On Day 5, Mean 1, 3-Propene Sultone Concentration (mg/L) |
Degradation (%) |
Sample pH 4 |
10.43 |
BLOQ |
100 |
Sample pH 7 |
10.11 |
BLOQ |
100 |
Sample pH 9 |
10.05 |
BLOQ |
100 |
BLOQ: Below Limit of quantification
TABLE 1. CONCENTRATION OF1, 3-PROPENE SULTONEIN pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 0 HOUR
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 0 hour |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 0 hour |
276473 |
5.0500 |
2.0 |
9.37 |
9.37 |
|
Sample pH 4 R2 0 hour |
276481 |
5.0500 |
2.0 |
9.37 |
||
Control pH 7 0 hour |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 0 hour |
275515 |
5.0500 |
2.0 |
9.33 |
9.30 |
|
Sample pH 7 R2 0 hour |
273364 |
5.0500 |
2.0 |
9.26 |
||
Control pH 9 0 hour |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 0 hour |
277751 |
5.0500 |
2.0 |
9.41 |
9.38 |
|
Sample pH 9 R2 0 hour |
275898 |
5.0500 |
2.0 |
9.35 |
TABLE 2. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 4thHOUR INCUBATED AT 15°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 4 hrs 15D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 4 hrs 15D |
274945 |
5.0500 |
2.0 |
9.31 |
9.33 |
|
Sample pH 4 R2 4 hrs 15D |
275672 |
5.0500 |
2.0 |
9.34 |
||
Control pH 7 4 hrs 15D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 4 hrs 15D |
272521 |
5.0500 |
2.0 |
9.23 |
9.23 |
|
Sample pH 7 R2 4 hrs 15D |
272408 |
5.0500 |
2.0 |
9.23 |
||
Control pH 9 4 hrs 15D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 4 hrs 15D |
276583 |
5.0500 |
2.0 |
9.37 |
9.33 |
|
Sample pH 9 R2 4 hrs 15D |
274265 |
5.0500 |
2.0 |
9.29 |
TABLE 3. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 4thHOUR INCUBATED AT 25°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 4 hrs 25 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 4 hrs 25 D |
272958 |
5.0500 |
2.0 |
9.25 |
9.21 |
|
Sample pH 4 R2 4 hrs 25 D |
270489 |
5.0500 |
2.0 |
9.16 |
||
Control pH 7 4 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 4 hrs 25 D |
269048 |
5.0500 |
2.0 |
9.12 |
9.13 |
|
Sample pH 7 R2 4 hrs 25 D |
269842 |
5.0500 |
2.0 |
9.14 |
||
Control pH 9 4 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 4 hrs 25 D |
272401 |
5.0500 |
2.0 |
9.23 |
9.26 |
|
Sample pH 9 R2 4 hrs 25 D |
274023 |
5.0500 |
2.0 |
9.28 |
TABLE 4. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 4thHOUR INCUBATED AT 50°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 4 hrs 50 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 4 hrs 50 D |
235552 |
5.0500 |
2.0 |
7.98 |
7.95 |
|
Sample pH 4 R2 4 hrs 50 D |
233917 |
5.0500 |
2.0 |
7.92 |
||
Control pH 7 4 hrs 50 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 4 hrs 50 D |
240817 |
5.0500 |
2.0 |
8.16 |
8.13 |
|
Sample pH 7 R2 4 hrs 50 D |
238652 |
5.0500 |
2.0 |
8.09 |
||
Control pH 9 4 hrs 50 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 4 hrs 50 D |
240595 |
5.0500 |
2.0 |
8.15 |
8.15 |
|
Sample pH 9 R2 4 hrs 50 D |
240183 |
5.0500 |
2.0 |
8.14 |
TABLE 5. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 8thHOUR INCUBATED AT 15°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 8 hrs 15 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 8 hrs 15 D |
270661 |
5.0500 |
2.0 |
9.17 |
9.19 |
|
Sample pH 4 R2 8 hrs 15 D |
271627 |
5.0500 |
2.0 |
9.20 |
||
Control pH 7 8 hrs 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 8 hrs 15 D |
271773 |
5.0500 |
2.0 |
9.21 |
9.21 |
|
Sample pH 7 R2 8 hrs 15 D |
271987 |
5.0500 |
2.0 |
9.21 |
||
Control pH 9 8 hrs 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 8 hrs 15 D |
273084 |
5.0500 |
2.0 |
9.25 |
9.25 |
|
Sample pH 9 R2 8 hrs 15 D |
272902 |
5.0500 |
2.0 |
9.25 |
TABLE 6. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 8thHOUR INCUBATED AT 25°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 8 hrs 25 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 8 hrs 25 D |
258287 |
5.0500 |
2.0 |
8.75 |
8.79 |
|
Sample pH 4 R2 8 hrs 25 D |
260302 |
5.0500 |
2.0 |
8.82 |
||
Control pH 7 8 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 8 hrs 25 D |
262270 |
5.0500 |
2.0 |
8.89 |
8.88 |
|
Sample pH 7 R2 8 hrs 25 D |
261664 |
5.0500 |
2.0 |
8.86 |
||
Control pH 9 8 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 8 hrs 25 D |
265632 |
5.0500 |
2.0 |
9.00 |
9.06 |
|
Sample pH 9 R2 8 hrs 25 D |
268952 |
5.0500 |
2.0 |
9.11 |
TABLE 7. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 8thHOUR INCUBATED AT 50°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 8 hrs 50 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 8 hrs 50 D |
212977 |
5.0500 |
2.0 |
7.22 |
7.27 |
|
Sample pH 4 R2 8 hrs 50 D |
215816 |
5.0500 |
2.0 |
7.31 |
||
Control pH 7 8 hrs 50 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 8 hrs 50 D |
228372 |
5.0500 |
2.0 |
7.74 |
7.76 |
|
Sample pH 7 R2 8 hrs 50 D |
229288 |
5.0500 |
2.0 |
7.77 |
||
Control pH 9 8 hrs 50 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 8 hrs 50 D |
224798 |
5.0500 |
2.0 |
7.62 |
7.61 |
|
Sample pH 9 R2 8 hrs 50 D |
224225 |
5.0500 |
2.0 |
7.60 |
TABLE 8. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 12thHOUR INCUBATED AT 15°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 12 hrs 15 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 12 hrs 15 D |
267634 |
5.0500 |
2.0 |
9.07 |
9.09 |
|
Sample pH 4 R2 12 hrs 15 D |
268690 |
5.0500 |
2.0 |
9.10 |
||
Control pH 7 12 hrs 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 12 hrs 15 D |
262699 |
5.0500 |
2.0 |
8.90 |
8.90 |
|
Sample pH 7 R2 12 hrs 15 D |
262428 |
5.0500 |
2.0 |
8.89 |
||
Control pH 9 12 hrs 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 12 hrs 15 D |
269245 |
5.0500 |
2.0 |
9.12 |
9.13 |
|
Sample pH 9 R2 12 hrs 15 D |
269807 |
5.0500 |
2.0 |
9.14 |
TABLE 9. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 12thHOUR INCUBATED AT 25°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 12 hrs 25 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 12 hrs 25 D |
250484 |
5.0500 |
2.0 |
8.49 |
8.59 |
|
Sample pH 4 R2 12 hrs 25 D |
256608 |
5.0500 |
2.0 |
8.69 |
||
Control pH 7 12 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 12 hrs 25 D |
248234 |
5.0500 |
2.0 |
8.41 |
8.36 |
|
Sample pH 7 R2 12 hrs 25 D |
245004 |
5.0500 |
2.0 |
8.30 |
||
Control pH 9 12 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 12 hrs 25 D |
253219 |
5.0500 |
2.0 |
8.58 |
8.62 |
|
Sample pH 9 R2 12 hrs 25 D |
255406 |
5.0500 |
2.0 |
8.65 |
TABLE 10. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 12thHOUR INCUBATED AT 50°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 12 hrs 50 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 12 hrs 50 D |
138825 |
5.0500 |
2.0 |
4.70 |
4.71 |
|
Sample pH 4 R2 12 hrs 50 D |
139310 |
5.0500 |
2.0 |
4.72 |
||
Control pH 7 12 hrs 50 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 12 hrs 50 D |
109447 |
5.0500 |
2.0 |
3.71 |
3.70 |
|
Sample pH 7 R2 12 hrs 50 D |
108532 |
5.0500 |
2.0 |
3.68 |
||
Control pH 9 12 hrs 50 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 12 hrs 50 D |
113817 |
5.0500 |
2.0 |
3.86 |
3.87 |
|
Sample pH 9 R2 12 hrs 50 D |
114652 |
5.0500 |
2.0 |
3.88 |
TABLE 11. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 20thHOUR INCUBATED AT 15°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 20 hrs 15 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 20 hrs 15 D |
265238 |
5.0500 |
2.0 |
8.99 |
9.02 |
|
Sample pH 4 R2 20 hrs 15 D |
266911 |
5.0500 |
2.0 |
9.04 |
||
Control pH 7 20 hrs 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 20 hrs 15 D |
258191 |
5.0500 |
2.0 |
8.75 |
8.83 |
|
Sample pH 7 R2 20 hrs 15 D |
262786 |
5.0500 |
2.0 |
8.90 |
||
Control pH 9 20 hrs 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 20 hrs 15 D |
265542 |
5.0500 |
2.0 |
9.00 |
8.97 |
|
Sample pH 9 R2 20 hrs 15 D |
263710 |
5.0500 |
2.0 |
8.93 |
TABLE 12. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 20thHOUR INCUBATED AT 25°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 20 hrs 25 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 20 hrs 25 D |
245068 |
5.0500 |
2.0 |
8.30 |
8.29 |
|
Sample pH 4 R2 20 hrs 25 D |
244439 |
5.0500 |
2.0 |
8.28 |
||
Control pH 7 20 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 20 hrs 25 D |
251930 |
5.0500 |
2.0 |
8.54 |
8.55 |
|
Sample pH 7 R2 20 hrs 25 D |
252686 |
5.0500 |
2.0 |
8.56 |
||
Control pH 9 20 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 20 hrs 25 D |
248840 |
5.0500 |
2.0 |
8.43 |
8.45 |
|
Sample pH 9 R2 20 hrs 25 D |
249657 |
5.0500 |
2.0 |
8.46 |
TABLE 13. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 20thHOUR INCUBATED AT 50°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 20 hrs 50 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 20 hrs 50 D |
38124 |
5.0500 |
2.0 |
1.29 |
1.28 |
|
Sample pH 4 R2 20 hrs 50 D |
37445 |
5.0500 |
2.0 |
1.27 |
||
Control pH 7 20 hrs 50 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 20 hrs 50 D |
36082 |
5.0500 |
2.0 |
1.22 |
1.22 |
|
Sample pH 7 R2 20 hrs 50 D |
35979 |
5.0500 |
2.0 |
1.22 |
||
Control pH 9 20 hrs 50 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 20 hrs 50 D |
33517 |
5.0500 |
2.0 |
1.14 |
1.14 |
|
Sample pH 9 R2 20 hrs 50 D |
33252 |
5.0500 |
2.0 |
1.13 |
TABLE 14. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 24thHOUR INCUBATED AT 15°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 24 hrs 15 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 24 hrs 15 D |
262425 |
5.0500 |
2.0 |
8.89 |
8.97 |
|
Sample pH 4 R2 24 hrs 15 D |
266796 |
5.0500 |
2.0 |
9.04 |
||
Control pH 7 24 hrs 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 24 hrs 15 D |
255572 |
5.0500 |
2.0 |
8.66 |
8.70 |
|
Sample pH 7 R2 24 hrs 15 D |
257620 |
5.0500 |
2.0 |
8.73 |
||
Control pH 9 24 hrs 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 24 hrs 15 D |
261338 |
5.0500 |
2.0 |
8.85 |
8.88 |
|
Sample pH 9 R2 24 hrs 15 D |
262856 |
5.0500 |
2.0 |
8.91 |
TABLE 15. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 24thHOUR INCUBATED AT 25°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 24 hrs 25 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 24 hrs 25 D |
232292 |
5.0500 |
2.0 |
7.87 |
7.92 |
|
Sample pH 4 R2 24 hrs 25 D |
235342 |
5.0500 |
2.0 |
7.97 |
||
Control pH 7 24 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 24 hrs 25 D |
217066 |
5.0500 |
2.0 |
7.35 |
7.47 |
|
Sample pH 7 R2 24 hrs 25 D |
224133 |
5.0500 |
2.0 |
7.59 |
||
Control pH 9 24 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 24 hrs 25 D |
235172 |
5.0500 |
2.0 |
7.97 |
7.90 |
|
Sample pH 9 R2 24 hrs 25 D |
231052 |
5.0500 |
2.0 |
7.83 |
TABLE 16. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 24thHOUR INCUBATED AT 50°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 24 hrs 50 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 24 hrs 50 D |
16113 |
5.0500 |
2.0 |
0.55 |
0.55 |
|
Sample pH 4 R2 24 hrs 50 D |
16073 |
5.0500 |
2.0 |
0.54 |
||
Control pH 7 24 hrs 50 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 24 hrs 50 D |
16974 |
5.0500 |
2.0 |
0.58 |
0.58 |
|
Sample pH 7 R2 24 hrs 50 D |
16805 |
5.0500 |
2.0 |
0.57 |
||
Control pH 9 24 hrs 50 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 24 hrs 50 D |
16523 |
5.0500 |
2.0 |
0.56 |
0.57 |
|
Sample pH 9 R2 24 hrs 50 D |
16732 |
5.0500 |
2.0 |
0.57 |
TABLE 17. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 30thHOUR INCUBATED AT 15°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 30 hrs 15 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 30 hrs 15 D |
248272 |
5.0500 |
2.0 |
8.41 |
8.33 |
|
Sample pH 4 R2 30 hrs 15 D |
243552 |
5.0500 |
2.0 |
8.25 |
||
Control pH 7 30 hrs 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 30 hrs 15 D |
245884 |
5.0500 |
2.0 |
8.33 |
8.38 |
|
Sample pH 7 R2 30 hrs 15 D |
248707 |
5.0500 |
2.0 |
8.43 |
||
Control pH 9 30 hrs 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 30 hrs 15 D |
253077 |
5.0500 |
2.0 |
8.57 |
8.58 |
|
Sample pH 9 R2 30 hrs 15 D |
253640 |
5.0500 |
2.0 |
8.59 |
TABLE 18. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 30thHOUR INCUBATED AT 25°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 30 hrs 25 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 30 hrs 25 D |
205478 |
5.0500 |
2.0 |
6.96 |
7.03 |
|
Sample pH 4 R2 30 hrs 25 D |
209595 |
5.0500 |
2.0 |
7.10 |
||
Control pH 7 30 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 30 hrs 25 D |
204334 |
5.0500 |
2.0 |
6.92 |
6.91 |
|
Sample pH 7 R2 30 hrs 25 D |
203456 |
5.0500 |
2.0 |
6.89 |
||
Control pH 9 30 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 30 hrs 25 D |
197928 |
5.0500 |
2.0 |
6.71 |
6.67 |
|
Sample pH 9 R2 30 hrs 25 D |
195425 |
5.0500 |
2.0 |
6.62 |
TABLE 19. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 30thHOUR INCUBATED AT 50°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 30 hrs 50 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 30 hrs 50 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
BLOQ |
|
Sample pH 4 R2 30 hrs 50 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
||
Control pH 7 30 hrs 50 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 30 hrs 50 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
BLOQ |
|
Sample pH 7 R2 30 hrs 50 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
||
Control pH 9 30 hrs 50 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 30 hrs 50 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
BLOQ |
|
Sample pH 9 R2 30 hrs 50 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
TABLE 20. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 48thHOUR INCUBATED AT 15°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 48 hrs 15 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 48 hrs 15 D |
242512 |
5.0500 |
2.0 |
8.22 |
8.18 |
|
Sample pH 4 R2 48 hrs 15 D |
240327 |
5.0500 |
2.0 |
8.14 |
||
Control pH 7 48 hrs 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 48 hrs 15 D |
235688 |
5.0500 |
2.0 |
7.98 |
7.94 |
|
Sample pH 7 R2 48 hrs 15 D |
232824 |
5.0500 |
2.0 |
7.89 |
||
Control pH 9 48 hrs 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 48 hrs 15 D |
242963 |
5.0500 |
2.0 |
8.23 |
8.20 |
|
Sample pH 9 R2 48 hrs 15 D |
240853 |
5.0500 |
2.0 |
8.16 |
TABLE 21. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 48thHOUR INCUBATED AT 25°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 48 hrs 25 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 48 hrs 25 D |
177072 |
5.0500 |
2.0 |
6.00 |
5.97 |
|
Sample pH 4 R2 48 hrs 25 D |
175195 |
5.0500 |
2.0 |
5.94 |
||
Control pH 7 48 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 48 hrs 25 D |
168500 |
5.0500 |
2.0 |
5.71 |
5.73 |
|
Sample pH 7 R2 48 hrs 25 D |
169504 |
5.0500 |
2.0 |
5.74 |
||
Control pH 9 48 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 48 hrs 25 D |
182696 |
5.0500 |
2.0 |
6.19 |
6.13 |
|
Sample pH 9 R2 48 hrs 25 D |
179009 |
5.0500 |
2.0 |
6.06 |
TABLE 22. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 48thHOUR INCUBATED AT 50°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 48 hrs 50 D |
BLOQ |
298122 |
- |
- |
- |
- |
Sample pH 4 R1 48 hrs 50 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
BLOQ |
|
Sample pH 4 R2 48 hrs 50 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
||
Control pH 7 48 hrs 50 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 48 hrs 50 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
BLOQ |
|
Sample pH 7 R2 48 hrs 50 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
||
Control pH 9 48 hrs 50 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 48 hrs 50 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
BLOQ |
|
Sample pH 9 R2 48 hrs 50 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
TABLE 23. SYSTEM SUITABILITY STANDARD (Date of Analysis: 11/12/2020)
Standard |
Peak Area (mAU) |
System Suitability Standard-Rep1 |
286751 |
System Suitability Standard-Rep2 |
285765 |
System Suitability Standard-Rep3 |
283806 |
System Suitability Standard-Rep4 |
284622 |
System Suitability Standard-Rep5 |
284386 |
Average |
285066 |
SD (±) |
1181 |
%RSD |
0.41 |
TABLE 24. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 72ndHOUR INCUBATED AT 15°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 72 hrs 15 D |
BLOQ |
285066 |
- |
- |
- |
- |
Sample pH 4 R1 72 hrs 15 D |
220822 |
5.0450 |
2.0 |
7.82 |
7.76 |
|
Sample pH 4 R2 72 hrs 15 D |
217508 |
5.0450 |
2.0 |
7.70 |
||
Control pH 7 72 hrs 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 72 hrs 15 D |
217869 |
5.0450 |
2.0 |
7.71 |
7.68 |
|
Sample pH 7 R2 72 hrs 15 D |
215937 |
5.0450 |
2.0 |
7.64 |
||
Control pH 9 72 hrs 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 72 hrs 15 D |
208082 |
5.0450 |
2.0 |
7.37 |
7.29 |
|
Sample pH 9 R2 72 hrs 15 D |
203783 |
5.0450 |
2.0 |
7.21 |
TABLE 25. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 72ndHOUR INCUBATED AT 25°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 72 hrs 25 D |
BLOQ |
285066 |
- |
- |
- |
- |
Sample pH 4 R1 72 hrs 25 D |
166713 |
5.0450 |
2.0 |
5.90 |
5.89 |
|
Sample pH 4 R2 72 hrs 25 D |
166175 |
5.0450 |
2.0 |
5.88 |
||
Control pH 7 72 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 72 hrs 25 D |
159118 |
5.0450 |
2.0 |
5.63 |
5.63 |
|
Sample pH 7 R2 72 hrs 25 D |
159196 |
5.0450 |
2.0 |
5.63 |
||
Control pH 9 72 hrs 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 72 hrs 25 D |
171494 |
5.0450 |
2.0 |
6.07 |
6.07 |
|
Sample pH 9 R2 72 hrs 25 D |
171370 |
5.0450 |
2.0 |
6.07 |
TABLE 26. SYSTEM SUITABILITY STANDARD (Date of Analysis: 14/12/2020)
Standard |
Peak Area (mAU) |
System Suitability Standard-Rep1 |
285943 |
System Suitability Standard-Rep2 |
285902 |
System Suitability Standard-Rep3 |
283806 |
System Suitability Standard-Rep4 |
284386 |
System Suitability Standard-Rep5 |
285382 |
Average |
285084 |
SD (±) |
951 |
%RSD |
0.33 |
TABLE 27. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 144thHOUR (DAY 6) INCUBATED AT 15°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 Day 6 15 D |
BLOQ |
285084 |
- |
- |
- |
- |
Sample pH 4 R1 Day 6 15 D |
184446 |
5.0400 |
2.0 |
6.52 |
6.43 |
|
Sample pH 4 R2 Day 6 15 D |
178900 |
5.0400 |
2.0 |
6.33 |
||
Control pH 7 Day 6 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 Day 6 15 D |
185136 |
5.0400 |
2.0 |
6.55 |
6.54 |
|
Sample pH 7 R2 Day 6 15 D |
184656 |
5.0400 |
2.0 |
6.53 |
||
Control pH 9 Day 6 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 Day 6 15 D |
197761 |
5.0400 |
2.0 |
6.99 |
6.92 |
|
Sample pH 9 R2 Day 6 15 D |
193463 |
5.0400 |
2.0 |
6.84 |
TABLE 28. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 144thHOUR (DAY 6) INCUBATED AT 25°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 Day 6 25 D |
BLOQ |
285084 |
- |
- |
- |
- |
Sample pH 4 R1 Day 6 25 D |
110527 |
5.0400 |
2.0 |
3.91 |
3.90 |
|
Sample pH 4 R2 Day 6 25 D |
109798 |
5.0400 |
2.0 |
3.88 |
||
Control pH 7 Day 6 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 Day 6 25 D |
82028 |
5.0400 |
2.0 |
2.90 |
2.95 |
|
Sample pH 7 R2 Day 6 25 D |
84670 |
5.0400 |
2.0 |
2.99 |
||
Control pH 9 Day 6 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 Day 6 25 D |
103795 |
5.0400 |
2.0 |
3.67 |
3.69 |
|
Sample pH 9 R2 Day 6 25 D |
105016 |
5.0400 |
2.0 |
3.71 |
TABLE 29. SYSTEM SUITABILITY STANDARD (Date of Analysis: 16/12/2020)
Standard |
Peak Area (mAU) |
System Suitability Standard-Rep1 |
248744 |
System Suitability Standard-Rep2 |
246580 |
System Suitability Standard-Rep3 |
243967 |
System Suitability Standard-Rep4 |
237911 |
System Suitability Standard-Rep5 |
245357 |
Average |
244512 |
SD (±) |
4086 |
%RSD |
1.67 |
TABLE 30. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 192ndHOUR (DAY 8) INCUBATED AT 25°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 Day 8 25 D |
BLOQ |
244512 |
- |
- |
- |
- |
Sample pH 4 R1 Day 8 25 D |
64676 |
4.9100 |
2.0 |
2.60 |
2.57 |
|
Sample pH 4 R2 Day 8 25 D |
62905 |
4.9100 |
2.0 |
2.53 |
||
Control pH 7 Day 8 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 Day 8 25 D |
56917 |
4.9100 |
2.0 |
2.29 |
2.26 |
|
Sample pH 7 R2 Day 8 25 D |
55529 |
4.9100 |
2.0 |
2.23 |
||
Control pH 9 Day 8 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 Day 8 25 D |
65684 |
4.9100 |
2.0 |
2.64 |
2.68 |
|
Sample pH 9 R2 Day 8 25 D |
67661 |
4.9100 |
2.0 |
2.72 |
TABLE 31. SYSTEM SUITABILITY STANDARD (Date of Analysis: 19/12/2020)
Standard |
Peak Area (mAU) |
System Suitability Standard-Rep1 |
291402 |
System Suitability Standard-Rep2 |
294584 |
System Suitability Standard-Rep3 |
287031 |
System Suitability Standard-Rep4 |
293566 |
System Suitability Standard-Rep5 |
289161 |
Average |
291149 |
SD (±) |
3107 |
%RSD |
1.07 |
TABLE 32. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 264thHOUR (DAY 11) INCUBATED AT 25°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 Day 11 25 D |
BLOQ |
291149 |
- |
- |
- |
- |
Sample pH 4 R1 Day 11 25 D |
34260 |
5.0300 |
2.0 |
1.18 |
1.19 |
|
Sample pH 4 R2 Day 11 25 D |
34796 |
5.0300 |
2.0 |
1.20 |
||
Control pH 7 Day 11 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 Day 11 25 D |
33278 |
5.0300 |
2.0 |
1.15 |
1.16 |
|
Sample pH 7 R2 Day 11 25 D |
33467 |
5.0300 |
2.0 |
1.16 |
||
Control pH 9 Day 11 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 Day 11 25 D |
34785 |
5.0300 |
2.0 |
1.20 |
1.24 |
|
Sample pH 9 R2 Day 11 25 D |
36906 |
5.0300 |
2.0 |
1.28 |
TABLE 33. SYSTEM SUITABILITY STANDARD (Date of Analysis: 23/12/2020)
Standard |
Peak Area (mAU) |
System Suitability Standard-Rep1 |
264071 |
System Suitability Standard-Rep2 |
261685 |
System Suitability Standard-Rep3 |
261730 |
System Suitability Standard-Rep4 |
253785 |
System Suitability Standard-Rep5 |
255037 |
Average |
259262 |
SD (±) |
4553 |
%RSD |
1.76 |
TABLE 34. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 360thHOUR (DAY 15) INCUBATED AT 15°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 Day 15 15 D |
BLOQ |
259262 |
- |
- |
- |
- |
Sample pH 4 R1 Day 15 15 D |
107531 |
5.0500 |
2.0 |
4.19 |
4.17 |
|
Sample pH 4 R2 Day 15 15 D |
106537 |
5.0500 |
2.0 |
4.15 |
||
Control pH 7 Day 15 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 Day 15 15 D |
105149 |
5.0500 |
2.0 |
4.10 |
4.10 |
|
Sample pH 7 R2 Day 15 15 D |
105126 |
5.0500 |
2.0 |
4.10 |
||
Control pH 9 Day 15 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 Day 15 15 D |
122089 |
5.0500 |
2.0 |
4.76 |
4.75 |
|
Sample pH 9 R2 Day 15 15 D |
121665 |
5.0500 |
2.0 |
4.74 |
TABLE 35. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 360thHOUR (DAY 15) INCUBATED AT 25°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 Day 15 25 D |
BLOQ |
259262 |
- |
- |
- |
- |
Sample pH 4 R1 Day 15 25 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
BLOQ |
|
Sample pH 4 R2 Day 15 25 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
||
Control pH 7 Day 15 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 Day 15 25 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
BLOQ |
|
Sample pH 7 R2 Day 15 25 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
||
Control pH 9 Day 15 25 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 Day 15 25 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
BLOQ |
|
Sample pH 9 R2 Day 15 25 D |
BLOQ |
5.0500 |
2.0 |
BLOQ |
TABLE 36. SYSTEM SUITABILITY STANDARD (Date of Analysis: 30/12/2020)
Standard |
Peak Area (mAU) |
System Suitability Standard-Rep1 |
281723 |
System Suitability Standard-Rep2 |
282081 |
System Suitability Standard-Rep3 |
285611 |
System Suitability Standard-Rep4 |
285876 |
System Suitability Standard-Rep5 |
284892 |
Average |
284037 |
SD (±) |
1986 |
%RSD |
0.70 |
TABLE 37. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 528thHOUR (DAY 22) INCUBATED AT 15°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 Day 22 15 D |
BLOQ |
284037 |
- |
- |
- |
- |
Sample pH 4 R1 Day 22 15 D |
77047 |
5.0550 |
2.0 |
2.74 |
2.72 |
|
Sample pH 4 R2 Day 22 15 D |
75740 |
5.0550 |
2.0 |
2.70 |
||
Control pH 7 Day 22 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 Day 22 15 D |
73276 |
5.0550 |
2.0 |
2.61 |
2.58 |
|
Sample pH 7 R2 Day 22 15 D |
71364 |
5.0550 |
2.0 |
2.54 |
||
Control pH 9 Day 22 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 Day 22 15 D |
75604 |
5.0550 |
2.0 |
2.69 |
2.71 |
|
Sample pH 9 R2 Day 22 15 D |
76635 |
5.0550 |
2.0 |
2.73 |
TABLE 38. SYSTEM SUITABILITY STANDARD (Date of Analysis: 07/01/2021)
Standard |
Peak Area (mAU) |
System Suitability Standard-Rep1 |
281272 |
System Suitability Standard-Rep2 |
280479 |
System Suitability Standard-Rep3 |
281673 |
System Suitability Standard-Rep4 |
277167 |
System Suitability Standard-Rep5 |
271710 |
Average |
278460 |
SD (±) |
4170 |
% RSD |
1.50 |
TABLE 39. CONCENTRATION OF1, 3-PROPENE SULTONEIN THE pH 4, pH 7 AND pH 9 BUFFER SOLUTIONS AT 720thHOUR (DAY 30) INCUBATED AT 15°C
Sample Code |
Peak Area (mAU) |
Mean Standard Peak Area (mAU) |
Standard Conc. (mg/L) |
Dilution Factor |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Control pH 4 Day 30 15 D |
BLOQ |
278460 |
- |
- |
- |
- |
Sample pH 4 R1 Day 30 15 D |
53354 |
5.0650 |
2.0 |
1.94 |
1.92 |
|
Sample pH 4 R2 Day 30 15 D |
52279 |
5.0650 |
2.0 |
1.90 |
||
Control pH 7 Day 30 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 7 R1 Day 30 15 D |
50461 |
5.0650 |
2.0 |
1.84 |
1.85 |
|
Sample pH 7 R2 Day 30 15 D |
51078 |
5.0650 |
2.0 |
1.86 |
||
Control pH 9 Day 30 15 D |
BLOQ |
- |
- |
- |
- |
|
Sample pH 9 R1 Day 30 15 D |
54636 |
5.0650 |
2.0 |
1.99 |
1.98 |
|
Sample pH 9 R2 Day 30 15 D |
54280 |
5.0650 |
2.0 |
1.97 |
TABLE 40. TIER 2 - HYDROLYSIS RATE CONSTANT AND HALF-LIFE OF1, 3-PROPENE SULTONE INpH 4 BUFFER SAMPLES INCUBATED AT 15°C
Occasion (Hours) |
Sample Code |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Log of Mean Concentration |
Rate Constant (Kobs)hour-1 |
0 |
Sample pH 4 R1 0 hour |
9.37 |
9.37 |
0.9717 |
- |
Sample pH 4 R2 0 hour |
9.37 |
||||
4.0 |
Sample pH 4 R1 4 hours |
9.31 |
9.33 |
0.9699 |
0.0011 |
Sample pH 4 R2 4 hours |
9.34 |
||||
8.0 |
Sample pH 4 R1 8 hours |
9.17 |
9.19 |
0.9633 |
0.0024 |
Sample pH 4 R2 8 hours |
9.20 |
||||
12.0 |
Sample pH 4 R1 12 hours |
9.07 |
9.09 |
0.9586 |
0.0025 |
Sample pH 4 R2 12 hours |
9.10 |
||||
20.0 |
Sample pH 4 R1 20 hours |
8.99 |
9.02 |
0.9552 |
0.0019 |
Sample pH 4 R2 20 hours |
9.04 |
||||
24.0 |
Sample pH 4 R1 24 hours |
8.89 |
8.97 |
0.9528 |
0.0018 |
Sample pH 4 R2 24 hours |
9.04 |
||||
30.0 |
Sample pH 4 R1 30 hours |
8.41 |
8.33 |
0.9206 |
0.0039 |
Sample pH 4 R2 30 hours |
8.25 |
||||
48.0 |
Sample pH 4 R1 48 hours |
8.22 |
8.18 |
0.9128 |
0.0028 |
Sample pH 4 R2 48 hours |
8.14 |
||||
72.0 |
Sample pH 4 R1 72 hours |
7.82 |
7.76 |
0.8899 |
0.0026 |
Sample pH 4 R2 72 hours |
7.70 |
||||
144.0 |
Sample pH 4 R1 144 hours |
6.52 |
6.43 |
0.8082 |
0.0026 |
Sample pH 4 R2 144 hours |
6.33 |
||||
360.0 |
Sample pH 4 R1 360 hours |
4.19 |
4.17 |
0.6201 |
0.0022 |
Sample pH 4 R2 360 hours |
4.15 |
||||
528.0 |
Sample pH 4 R1 528 hours |
2.74 |
2.72 |
0.4346 |
0.0023 |
Sample pH 4 R2 528 hours |
2.70 |
||||
720.0 |
Sample pH 4 R1 720 hours |
1.94 |
1.92 |
0.2833 |
0.0022 |
Sample pH 4 R2 720 hours |
1.90 |
||||
Mean Rate Constant (Kobs)hour-1 |
0.0024 |
||||
Half-life (t1/2) hour |
288.75 |
TABLE 41. TIER 2 - HYDROLYSIS RATE CONSTANT AND HALF-LIFE OF1, 3-PROPENE SULTONE INpH 7 BUFFER SAMPLES INCUBATED AT 15°C
Occasion (Hours) |
Sample Code |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Log of Mean Concentration |
Rate Constant (Kobs)hour-1 |
0 |
Sample pH 7 R1 0 hour |
9.33 |
9.30 |
0.9685 |
- |
Sample pH 7 R2 0 hour |
9.26 |
||||
4.0 |
Sample pH 7 R1 4 hours |
9.23 |
9.23 |
0.9652 |
0.0019 |
Sample pH 7 R2 4 hours |
9.23 |
||||
8.0 |
Sample pH 7 R1 8 hours |
9.21 |
9.21 |
0.9643 |
0.0012 |
Sample pH 7 R2 8 hours |
9.21 |
||||
12.0 |
Sample pH 7 R1 12 hours |
8.90 |
8.90 |
0.9494 |
0.0037 |
Sample pH 7 R2 12 hours |
8.89 |
||||
20.0 |
Sample pH 7 R1 20 hours |
8.75 |
8.83 |
0.9460 |
0.0026 |
Sample pH 7 R2 20 hours |
8.90 |
||||
24.0 |
Sample pH 7 R1 24 hours |
8.66 |
8.70 |
0.9395 |
0.0028 |
Sample pH 7 R2 24 hours |
8.73 |
||||
30.0 |
Sample pH 7 R1 30 hours |
8.33 |
8.38 |
0.9232 |
0.0035 |
Sample pH 7 R2 30 hours |
8.43 |
||||
48.0 |
Sample pH 7 R1 48 hours |
7.98 |
7.94 |
0.8998 |
0.0033 |
Sample pH 7 R2 48 hours |
7.89 |
||||
72.0 |
Sample pH 7 R1 72 hours |
7.71 |
7.68 |
0.8854 |
0.0027 |
Sample pH 7 R2 72 hours |
7.64 |
||||
144.0 |
Sample pH 7 R1 144 hours |
6.55 |
6.54 |
0.8156 |
0.0024 |
Sample pH 7 R2 144 hours |
6.53 |
||||
360.0 |
Sample pH 7 R1 360 hours |
4.10 |
4.10 |
0.6128 |
0.0023 |
Sample pH 7 R2 360 hours |
4.10 |
||||
528.0 |
Sample pH 7 R1 528 hours |
2.61 |
2.58 |
0.4116 |
0.0024 |
Sample pH 7 R2 528 hours |
2.54 |
||||
720.0 |
Sample pH 7 R1 720 hours |
1.84 |
1.85 |
0.2672 |
0.0022 |
Sample pH 7 R2 720 hours |
1.86 |
||||
Mean Rate Constant (Kobs)hour-1 |
0.0026 |
||||
Half-life (t1/2) hour |
266.54 |
TABLE 42. TIER 2 - HYDROLYSIS RATE CONSTANT AND HALF-LIFE OF1, 3-PROPENE SULTONE INpH 9 BUFFER SAMPLES INCUBATED AT 15°C
Occasion (Hours) |
Sample Code |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Log of Mean Concentration |
Rate Constant (Kobs)hour-1 |
0 |
Sample pH 9 R1 0 hour |
9.41 |
9.38 |
0.9722 |
- |
Sample pH 9 R2 0 hour |
9.35 |
||||
4.0 |
Sample pH 9 R1 4 hours |
9.37 |
9.33 |
0.9699 |
0.0013 |
Sample pH 9 R2 4 hours |
9.29 |
||||
8.0 |
Sample pH 9 R1 8 hours |
9.25 |
9.25 |
0.9661 |
0.0017 |
Sample pH 9 R2 8 hours |
9.25 |
||||
12.0 |
Sample pH 9 R1 12 hours |
9.12 |
9.13 |
0.9605 |
0.0023 |
Sample pH 9 R2 12 hours |
9.14 |
||||
20.0 |
Sample pH 9 R1 20 hours |
9.00 |
8.97 |
0.9528 |
0.0022 |
Sample pH 9 R2 20 hours |
8.93 |
||||
24.0 |
Sample pH 9 R1 24 hours |
8.85 |
8.88 |
0.9484 |
0.0023 |
Sample pH 9 R2 24 hours |
8.91 |
||||
30.0 |
Sample pH 9 R1 30 hours |
8.57 |
8.58 |
0.9335 |
0.0030 |
Sample pH 9 R2 30 hours |
8.59 |
||||
48.0 |
Sample pH 9 R1 48 hours |
8.23 |
8.20 |
0.9138 |
0.0028 |
Sample pH 9 R2 48 hours |
8.16 |
||||
72.0 |
Sample pH 9 R1 72 hours |
7.37 |
7.29 |
0.8627 |
0.0035 |
Sample pH 9 R2 72 hours |
7.21 |
||||
144.0 |
Sample pH 9 R1 144 hours |
6.99 |
6.92 |
0.8401 |
0.0021 |
Sample pH 9 R2 144 hours |
6.84 |
||||
360.0 |
Sample pH 9 R1 360 hours |
4.76 |
4.75 |
0.6767 |
0.0019 |
Sample pH 9 R2 360 hours |
4.74 |
||||
528.0 |
Sample pH 9 R1 528 hours |
2.69 |
2.71 |
0.4330 |
0.0024 |
Sample pH 9 R2 528 hours |
2.73 |
||||
720.0 |
Sample pH 9 R1 720 hours |
1.99 |
1.98 |
0.2967 |
0.0022 |
Sample pH 9 R2 720 hours |
1.97 |
||||
Mean Rate Constant (Kobs)hour-1 |
0.0023 |
||||
Half-life (t1/2) hour |
301.30 |
TABLE 43. TIER 2 - HYDROLYSIS RATE CONSTANT AND HALF-LIFE OF1, 3-PROPENE SULTONE INpH 4 BUFFER SAMPLES INCUBATED AT 25°C
Occasion (Hours) |
Sample Code |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Log of Mean Concentration |
Rate Constant (Kobs)hour-1 |
0 |
Sample pH 4 R1 0 hour |
9.37 |
9.37 |
0.9717 |
- |
Sample pH 4 R2 0 hour |
9.37 |
||||
4.0 |
Sample pH 4 R1 4 hours |
9.25 |
9.21 |
0.9643 |
0.0043 |
Sample pH 4 R2 4 hours |
9.16 |
||||
8.0 |
Sample pH 4 R1 8 hours |
8.75 |
8.79 |
0.9440 |
0.0080 |
Sample pH 4 R2 8 hours |
8.82 |
||||
12.0 |
Sample pH 4 R1 12 hours |
8.49 |
8.59 |
0.9340 |
0.0072 |
Sample pH 4 R2 12 hours |
8.69 |
||||
20.0 |
Sample pH 4 R1 20 hours |
8.30 |
8.29 |
0.9186 |
0.0061 |
Sample pH 4 R2 20 hours |
8.28 |
||||
24.0 |
Sample pH 4 R1 24 hours |
7.87 |
7.92 |
0.8987 |
0.0070 |
Sample pH 4 R2 24 hours |
7.97 |
||||
30.0 |
Sample pH 4 R1 30 hours |
6.96 |
7.03 |
0.8470 |
0.0096 |
Sample pH 4 R2 30 hours |
7.10 |
||||
48.0 |
Sample pH 4 R1 48 hours |
6.00 |
5.97 |
0.7760 |
0.0094 |
Sample pH 4 R2 48 hours |
5.94 |
||||
72.0 |
Sample pH 4 R1 72 hours |
5.90 |
5.89 |
0.7701 |
0.0064 |
Sample pH 4 R2 72 hours |
5.88 |
||||
144.0 |
Sample pH 4 R1 144 hours |
3.91 |
3.90 |
0.5911 |
0.0061 |
Sample pH 4 R2 144 hours |
3.88 |
||||
192.0 |
Sample pH 4 R1 192 hours |
2.60 |
2.57 |
0.4099 |
0.0067 |
Sample pH 4 R2 192 hours |
2.53 |
||||
264.0 |
Sample pH 4 R1 264 hours |
1.18 |
1.19 |
0.0755 |
0.0078 |
Sample pH 4 R2 264 hours |
1.20 |
||||
360.0 |
Sample pH 4 R1 360 hours |
0.00 |
0.00 |
0.0000 |
0.0000 |
Sample pH 4 R2 360 hours |
0.00 |
||||
Mean Rate Constant (Kobs)hour-1 |
0.0071 |
||||
Half-life (t1/2) hour |
97.61 |
TABLE 44. TIER 2 - HYDROLYSIS RATE CONSTANT AND HALF-LIFE OF1, 3-PROPENE SULTONE INpH 7 BUFFER SAMPLES INCUBATED AT 25°C
Occasion (Hours) |
Sample Code |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Log of Mean Concentration |
Rate Constant (Kobs)hour-1 |
0 |
Sample pH 7 R1 0 hour |
9.33 |
9.30 |
0.9685 |
- |
Sample pH 7 R2 0 hour |
9.26 |
||||
4.0 |
Sample pH 7 R1 4 hours |
9.12 |
9.13 |
0.9605 |
0.0046 |
Sample pH 7 R2 4 hours |
9.14 |
||||
8.0 |
Sample pH 7 R1 8 hours |
8.89 |
8.88 |
0.9484 |
0.0058 |
Sample pH 7 R2 8 hours |
8.86 |
||||
12.0 |
Sample pH 7 R1 12 hours |
8.41 |
8.36 |
0.9222 |
0.0089 |
Sample pH 7 R2 12 hours |
8.30 |
||||
20.0 |
Sample pH 7 R1 20 hours |
8.54 |
8.55 |
0.9320 |
0.0042 |
Sample pH 7 R2 20 hours |
8.56 |
||||
24.0 |
Sample pH 7 R1 24 hours |
7.35 |
7.47 |
0.8733 |
0.0091 |
Sample pH 7 R2 24 hours |
7.59 |
||||
30.0 |
Sample pH 7 R1 30 hours |
6.92 |
6.91 |
0.8395 |
0.0099 |
Sample pH 7 R2 30 hours |
6.89 |
||||
48.0 |
Sample pH 7 R1 48 hours |
5.71 |
5.73 |
0.7582 |
0.0101 |
Sample pH 7 R2 48 hours |
5.74 |
||||
72.0 |
Sample pH 7 R1 72 hours |
5.63 |
5.63 |
0.7505 |
0.0070 |
Sample pH 7 R2 72 hours |
5.63 |
||||
144.0 |
Sample pH 7 R1 144 hours |
2.90 |
2.95 |
0.4698 |
0.0080 |
Sample pH 7 R2 144 hours |
2.99 |
||||
192.0 |
Sample pH 7 R1 192 hours |
2.29 |
2.26 |
0.3541 |
0.0074 |
Sample pH 7 R2 192 hours |
2.23 |
||||
264.0 |
Sample pH 7 R1 264 hours |
1.15 |
1.16 |
0.0645 |
0.0079 |
Sample pH 7 R2 264 hours |
1.16 |
||||
360.0 |
Sample pH 7 R1 360 hours |
0.00 |
0.00 |
0.0000 |
0.0000 |
Sample pH 7 R2 360 hours |
0.00 |
||||
Mean Rate Constant (Kobs)hour-1 |
0.0075 |
||||
Half-life (t1/2) hour |
92.40 |
TABLE 45. TIER-2 - HYDROLYSIS RATE CONSTANT AND HALF-LIFE OF1, 3-PROPENE SULTONE INpH 9 BUFFER SAMPLES INCUBATED AT 25°C
Occasion (Hours) |
Sample Code |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Log of Mean Concentration |
Rate Constant (Kobs)hour-1 |
0 |
Sample pH 9 R1 0 hour |
9.41 |
9.38 |
0.9722 |
- |
Sample pH 9 R2 0 hour |
9.35 |
||||
4.0 |
Sample pH 9 R1 4 hours |
9.23 |
9.26 |
0.9666 |
0.0032 |
Sample pH 9 R2 4 hours |
9.28 |
||||
8.0 |
Sample pH 9 R1 8 hours |
9.00 |
9.06 |
0.9571 |
0.0043 |
Sample pH 9 R2 8 hours |
9.11 |
||||
12.0 |
Sample pH 9 R1 12 hours |
8.58 |
8.62 |
0.9355 |
0.0070 |
Sample pH 9 R2 12 hours |
8.65 |
||||
20.0 |
Sample pH 9 R1 20 hours |
8.43 |
8.45 |
0.9269 |
0.0052 |
Sample pH 9 R2 20 hours |
8.46 |
||||
24.0 |
Sample pH 9 R1 24 hours |
7.97 |
7.90 |
0.8976 |
0.0072 |
Sample pH 9 R2 24 hours |
7.83 |
||||
30.0 |
Sample pH 9 R1 30 hours |
6.71 |
6.67 |
0.8241 |
0.0114 |
Sample pH 9 R2 30 hours |
6.62 |
||||
48.0 |
Sample pH 9 R1 48 hours |
6.19 |
6.13 |
0.7875 |
0.0089 |
Sample pH 9 R2 48 hours |
6.06 |
||||
72.0 |
Sample pH 9 R1 72 hours |
6.07 |
6.07 |
0.7832 |
0.0060 |
Sample pH 9 R2 72 hours |
6.07 |
||||
144.0 |
Sample pH 9 R1 144 hours |
3.67 |
3.69 |
0.5670 |
0.0065 |
Sample pH 9 R2 144 hours |
3.71 |
||||
192.0 |
Sample pH 9 R1 192 hours |
2.64 |
2.68 |
0.4281 |
0.0065 |
Sample pH 9 R2 192 hours |
2.72 |
||||
264.0 |
Sample pH 9 R1 264 hours |
1.20 |
1.24 |
0.0934 |
0.0077 |
Sample pH 9 R2 264 hours |
1.28 |
||||
360.0 |
Sample pH 9 R1 360 hours |
0.00 |
0.00 |
0.0000 |
0.0000 |
Sample pH 9 R2 360 hours |
0.00 |
||||
Mean Rate Constant (Kobs)hour-1 |
0.0067 |
||||
Half-life (t1/2) hour |
103.43 |
TABLE 46. TIER 2 - HYDROLYSIS RATE CONSTANT AND HALF-LIFE OF1, 3-PROPENE SULTONE INpH 4 BUFFER SAMPLES INCUBATED AT 50°C
Occasion (Hours) |
Sample Code |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Log of Mean Concentration |
Rate Constant (Kobs)hour-1 |
0 |
Sample pH 4 R1 0 hour |
9.37 |
9.37 |
0.9717 |
- |
Sample pH 4 R2 0 hour |
9.37 |
||||
4.0 |
Sample pH 4 R1 4 hours |
7.98 |
7.95 |
0.9004 |
0.0411 |
Sample pH 4 R2 4 hours |
7.92 |
||||
8.0 |
Sample pH 4 R1 8 hours |
7.22 |
7.27 |
0.8615 |
0.0317 |
Sample pH 4 R2 8 hours |
7.31 |
||||
12.0 |
Sample pH 4 R1 12 hours |
4.70 |
4.71 |
0.6730 |
0.0573 |
Sample pH 4 R2 12 hours |
4.72 |
||||
20.0 |
Sample pH 4 R1 20 hours |
1.29 |
1.28 |
0.1072 |
0.0996 |
Sample pH 4 R2 20 hours |
1.27 |
||||
24.0 |
Sample pH 4 R1 24 hours |
0.55 |
0.55 |
-0.2596 |
0.1182 |
Sample pH 4 R2 24 hours |
0.54 |
||||
30.0 |
Sample pH 4 R1 30 hours |
0.00 |
0.00 |
0.0000 |
0.0000 |
Sample pH 4 R2 30 hours |
0.00 |
||||
48.0 |
Sample pH 4 R1 48 hours |
0.00 |
0.00 |
0.0000 |
0.0000 |
Sample pH 4 R2 48 hours |
0.00 |
||||
Mean Rate Constant (Kobs)hour-1 |
0.0696 |
||||
Half-life (t1/2) hour |
9.96 |
TABLE 47. TIER-2 - HYDROLYSIS RATE CONSTANT AND HALF-LIFE OF1, 3-PROPENE SULTONE INpH 7 BUFFER SAMPLES INCUBATED AT 50°C
Occasion (Hours) |
Sample Code |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Log of Mean Concentration |
Rate Constant (Kobs)hour-1 |
0 |
Sample pH 7 R1 0 hour |
9.33 |
9.30 |
0.9685 |
- |
Sample pH 7 R2 0 hour |
9.26 |
||||
4.0 |
Sample pH 7 R1 4 hours |
8.16 |
8.13 |
0.9101 |
0.0336 |
Sample pH 7 R2 4 hours |
8.09 |
||||
8.0 |
Sample pH 7 R1 8 hours |
7.74 |
7.76 |
0.8899 |
0.0226 |
Sample pH 7 R2 8 hours |
7.77 |
||||
12.0 |
Sample pH 7 R1 12 hours |
3.71 |
3.70 |
0.5682 |
0.0768 |
Sample pH 7 R2 12 hours |
3.68 |
||||
20.0 |
Sample pH 7 R1 20 hours |
1.22 |
1.22 |
0.0864 |
0.1016 |
Sample pH 7 R2 20 hours |
1.22 |
||||
24.0 |
Sample pH 7 R1 24 hours |
0.58 |
0.58 |
-0.2366 |
0.1156 |
Sample pH 7 R2 24 hours |
0.57 |
||||
30.0 |
Sample pH 7 R1 30 hours |
0.00 |
0.00 |
0.0000 |
0.0000 |
Sample pH 7 R2 30 hours |
0.00 |
||||
48.0 |
Sample pH 7 R1 48 hours |
0.00 |
0.00 |
0.0000 |
0.0000 |
Sample pH 7 R2 48 hours |
0.00 |
||||
Mean Rate Constant (Kobs)hour-1 |
0.0700 |
||||
Half-life (t1/2) hour |
9.90 |
TABLE 48. TIER-2 - HYDROLYSIS RATE CONSTANT AND HALF-LIFE OF1, 3-PROPENE SULTONE INpH 9 BUFFER SAMPLES INCUBATED AT 50°C
Occasion (Hours) |
Sample Code |
Obtained Concentration (mg/L) |
Mean Obtained Concentration (mg/L) |
Log of Mean Concentration |
Mean Rate Constant (Kobs)hour-1 |
0 |
Sample pH 9 R1 0 hour |
9.41 |
9.38 |
0.9722 |
- |
Sample pH 9 R2 0 hour |
9.35 |
||||
4.0 |
Sample pH 9 R1 4 hours |
8.15 |
8.15 |
0.9112 |
0.0351 |
Sample pH 9 R2 4 hours |
8.14 |
||||
8.0 |
Sample pH 9 R1 8 hours |
7.62 |
7.61 |
0.8814 |
0.0261 |
Sample pH 9 R2 8 hours |
7.60 |
||||
12.0 |
Sample pH 9 R1 12 hours |
3.86 |
3.87 |
0.5877 |
0.0738 |
Sample pH 9 R2 12 hours |
3.88 |
||||
20.0 |
Sample pH 9 R1 20 hours |
1.14 |
1.14 |
0.0569 |
0.1054 |
Sample pH 9 R2 20 hours |
1.13 |
||||
24.0 |
Sample pH 9 R1 24 hours |
0.56 |
0.57 |
-0.2441 |
0.1167 |
Sample pH 9 R2 24 hours |
0.57 |
||||
30.0 |
Sample pH 9 R1 30 hours |
0.00 |
0.00 |
0.0000 |
0.0000 |
Sample pH 9 R2 30 hours |
0.00 |
||||
48.0 |
Sample pH 9 R1 48 hours |
0.00 |
0.00 |
0.0000 |
0.0000 |
Sample pH 9 R2 48 hours |
0.00 |
||||
Mean Rate Constant (Kobs)hour-1 |
0.0714 |
||||
Half-life (t1/2) hour |
9.71 |
Description of key information
The hydrolysis of the test item as a function of pH in aqueous solution was determined according to OECD guideline 111.
A preliminary test (Tier 1) was performed. The rate of hydrolysis at 50 ± 0.5°C was assessed for pH 4, 7 and 9.
Based on the results from the Preliminary test / Tier 1, further testing Tier 2 was conducted at pH 4, pH 7 and pH 9 with solutions being maintained at 15.0 ± 0.5°C, 25.0 ± 0.5°C and 50.0 ± 0.5°C for various time periods.
The half-life for hydrolysis of PST is described as below:
pH=4.0: Half-life are 288.75, 97.61, 9.96 h at temperature of 15, 25, 50 ℃, respectively.
pH=7.0: Half-life are 266.54, 92.40, 9.90 h at temperature of 15, 25, 50 ℃, respectively.
pH=9.0: Half-life are 301.30, 103.43, 9.71 h at temperature of 15, 25, 50 ℃, respectively.
Based on Tier 2 results, no other breakdown products are detected, hence tier 3 test for the identification of other products was not performed.
Key value for chemical safety assessment
- Half-life for hydrolysis:
- 92.4 h
- at the temperature of:
- 25 °C
Additional information
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Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
