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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1999
Report date:
1999

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes

Test material

Constituent 1
Reference substance name:
-
EC Number:
431-440-5
EC Name:
-
IUPAC Name:
(3-{5-[(E)-2-(3-{4-[(E)-2-{1-[3-(dimethylazaniumyl)propyl]-2-hydroxy-4-methyl-6-oxo-1,6-dihydropyridin-3-yl}diazen-1-yl]benzamido}phenyl)diazen-1-yl]-6-hydroxy-4-methyl-2-oxo-1,2-dihydropyridin-1-yl}propyl)dimethylazanium (3-{6-hydroxy-5-[(E)-2-(3-{4-[(E)-2-{2-hydroxy-4-methyl-1-[3-(methylazaniumyl)propyl]-6-oxo-1,6-dihydropyridin-3-yl}diazen-1-yl]benzamido}phenyl)diazen-1-yl]-4-methyl-2-oxo-1,2-dihydropyridin-1-yl}propyl)(methyl)azanium (3-{6-hydroxy-5-[(E)-2-(4-{3-[(E)-2-{2-hydroxy-4-methyl-1-[3-(methylazaniumyl)propyl]-6-oxo-1,6-dihydropyridin-3-yl}diazen-1-yl]benzamido}phenyl)diazen-1-yl]-4-methyl-2-oxo-1,2-dihydropyridin-1-yl}propyl)dimethylazanium hexaacetate

Test organisms

Test organisms (species):
activated sludge, domestic

Study design

Test type:
other: aerobic
Limit test:
no
Total exposure duration:
3 h

Test conditions

Test temperature:
21°C to 22°C
pH:
7.6 to 8.1
Dissolved oxygen:
7.8 to 8.6 mg O2/l /min
Nominal and measured concentrations:
nominal: 10, 32, 100, 320 and 1000 mg/l.
Reference substance (positive control):
yes

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
IC50
Effect conc.:
398 mg/L
Duration:
3 h
Dose descriptor:
NOEC
Effect conc.:
181 mg/L

Applicant's summary and conclusion