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EC number: 220-489-6 | CAS number: 2783-17-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 07. Nov. - 20. Dec. 2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- SOURCE OF TEST MATERIAL
- Source and lot/batch number of test material: B1-5-10
- Expiration date of the lot/batch: 31. Jan. 2020
- Purity test date: not stated
STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material: The test item was stored in the test facility in a closed vessel at room temperature (16.2 –
- Stability under storage conditions: assumed stable, no indication of the contrary
- Stability under test conditions: assumed stable, no indication of the contrary
- Solubility and stability of the test substance in the solvent/dispersant/vehicle/test medium: assumed stable and soluble, no indication of the contrary
- Reactivity of the test substance with the solvent/vehicle /test medium (if applicable): assumed stable, no indication of the contrary
TREATMENT OF TEST MATERIAL PRIOR TO TESTING
Preparation
A saturated solution was prepared. This was done by mixing the real load of 100.8 mg/L
with the corresponding amount of dilution water and shaking vigorously for 23.5 hours. The
resulting solution was filtrated through 0.45 µm PTFE filters. The lower treatments (4.6 / 10
/ 22 / 46 mg/L nominal) were prepared by dilution of this stock solution with dilution water.
The measured concentrations showed, that complete dissolution of the test item was
reached. - Analytical monitoring:
- yes
- Details on sampling:
- The pH, the concentration of dissolved oxygen and the content of the test item in the test
vessels were measured at the beginning and at the end of the test. - Vehicle:
- no
- Details on test solutions:
- A saturated solution was prepared. This was done by mixing the real load of 100.8 mg/L
with the corresponding amount of dilution water and shaking vigorously for 23.5 hours. The
resulting solution was filtrated through 0.45 µm PTFE filters. The lower treatments (4.6 / 10
/ 22 / 46 mg/L nominal) were prepared by dilution of this stock solution with dilution water.
The measured concentrations showed, that complete dissolution of the test item was
reached. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species Daphnia magna
Authority STRAUS
Strain Berlin
Sex female
Age between 0 and 24 hours
Origin Umweltbundesamt Berlin
In-house breeding since 27. September 2007 - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- according to guideline
- Post exposure observation period:
- no
- Hardness:
- 356 mg/L CaCO3
- Test temperature:
- 19.1 – 21.2 °C
- pH:
- 7.7
- Dissolved oxygen:
- 8.4 - 8.6 mg/L
- Salinity:
- not applicable
- Conductivity:
- not determined
- Nominal and measured concentrations:
- 4.6 / 10 / 22 / 46 / 100 mg/L (nominal concentration)
At the beginning and at the end of the test, the content of the test item in the test solutions
was determined using LC/MS-MS-determination. The measured concentrations lay be-
tween 96 % and 101 % of the nominal concentrations at the beginning of the test and be-
tween 108 % and 118 % of the nominal concentrations at the end of the test. Therefore,
the determination of the biological results was based on the nominal concentration - Details on test conditions:
- Date of performance 12. – 14. Nov. 2019
Treatments 4.6 / 10 / 22 / 46 / 100 mg/L (nominal concentration)
The concentrations to be tested are based on the result of
a non-GLP pre-test.
Temperature 19.1 – 21.2 °C
Photo period 16/8 hours, using neon tubes
Duration 48 hours
Observation times 24.5 and 48 hours
Medium renewal none
Test vessels glass beakers, nominal volume 50 mL, tall shape
Replicates (Treatments) 4 vessels, each containing 20 ± 5 mL test solution and 5
daphnia
Replicates (Blank control) 4 vessels, each containing 20 ± 5 mL dilution water and 5
daphnia - Reference substance (positive control):
- yes
- Key result
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 25.19 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 13.21 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- <= 4.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Results with reference substance (positive control):
- Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a
current reference study. The 24h-EC50 value was determined as 2.07 mg/L.
The value was within the requested range for the 24h-EC50 between 0.6 - 2.1 mg/L of po-
tassium dichromate K2Cr2O7 as specified in the OECD guideline. - Reported statistics and error estimates:
- The estimation of the biological results was accomplished using the software ToxRat®
Professional, version 3.3.0. The details of calculation are stated in the Annex 3: Statistical
calculation using ToxRat® Professional 3.3.0. - Validity criteria fulfilled:
- yes
- Conclusions:
The following results were determined for the test item 1,12-Diaminododecane (species:
Daphnia magna).
48h-NOEC = < 4.6 mg/L
48h-LOEC = ≤ 4.6 mg/L
24h-EC50 = 25.19 mg/L
48h-EC50 = 13.21 mg/L- Executive summary:
One valid experiment was performed.
The study was performed using 5 concentrations ranging from 4.6 to 100 mg/L nominal
concentration. For each test concentration and the blank control, 20 daphnia were ex-
posed to the test item for 48 hours in a static test system. After 24.5 and 48 hours, the im-
mobilised daphnia were counted.
Five concentrations showed toxicity between 15 and 100% immobilisation. None of the
animals was immobilised in the blank control.
Potassium dichromate K2Cr2O7 (CAS No. 7778-50-9) was used as positive control in a
current reference study to assure that the test conditions are reliable.
At the beginning and at the end of the test, the content of the test item in the test solutions
was determined using LC/MS-MS-determination. The measured concentrations lay be-
tween 96 % and 101 % of the nominal concentrations at the beginning of the test and be-
tween 108 % and 118 % of the nominal concentrations at the end of the test. Therefore,
the determination of the biological results was based on the nominal concentration (see
Guidance Doc. No.23 (§176)).
The following results were determined for the test item 1,12-Diaminododecane (species:
Daphnia magna).
48h-NOEC = < 4.6 mg/L
48h-LOEC = ≤ 4.6 mg/L
24h-EC50 = 25.19 mg/L
48h-EC50 = 13.21 mg/L
Reference
Description of key information
The following results were determined for the test item 1,12-Diaminododecane (species:
Daphnia magna).
48h-EC50 = 13.21 mg/L
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 13.21 mg/L
Additional information
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