Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 443-010-4 | CAS number: 53641-10-4
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2000-01-18 to 2000-03-01
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 000
- Report date:
- 2000
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- not specified
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- -
- EC Number:
- 443-010-4
- EC Name:
- -
- Cas Number:
- 53641-10-4
- Molecular formula:
- C14H15ClN2O4
- IUPAC Name:
- N-[3-chloro-4-(3-oxobutanamido)phenyl]-3-oxobutanamide
- Test material form:
- solid
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Aquaeous solutions
Volume: 20 mL
Nominal test substance concentrations: 0 mg/L; 22 mg/L; 50 mg/L;100 mg/L and
220 mg/L
Test solutions
- Vehicle:
- yes
- Remarks:
- Water
- Details on test solutions:
- A solubilizer was not used. The test substance was weighed,
water for dilution was added and was homogenized using an Ultra-Turrax.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- Species
Daphnia magna - Clone 5 (IRChA, France)
Origin
Stock culture of Aventis Pharma Deutschland GmbH, ProTox
Breeding conditions
100 mL beakers (height 90 mm, diameter 50 mm), filled with 70 mL test water (ac-cording to Elendt, M4) served as culture vessels. One daphnid was used per culture vessel. The animals were used for breeding up to an age of 42 days. The reproduction rate and the state of health of the animals were monitored daily apart from weekends. The animals were fed three times a week with monocellular green algae (Scenedes-mus subspicatus).
The supplied diet was based on the amount of organic carbon (C). The ration level is 0.2 mg carbon / day / animal.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 2.3 mmol Ca2+ + Mg2+ / L
- Test temperature:
- 20.2 - 20.6
- pH:
- 7.7 - 8.0
- Dissolved oxygen:
- 8.5 - 8.8
- Nominal and measured concentrations:
- Nominal: 22, 50, 100, 200 mg/L
Measured: 22.0, 54.3, 113.5, 223 mg/L - Details on test conditions:
- The study was conducted in a static system. As test chambers 250 mL beakers were used (height 110 mm, diameter 70 mm, water level about 75 mm), each filled with 200 mL test water. The room temperature was about 21°C. The room was illuminated by fluorescent tubes for 16 hours each day.
- Reference substance (positive control):
- no
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 69.9 mg/L
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 50.9 mg/L
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- 100 mg/L
- Duration:
- 48 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 22 mg/L
- Reported statistics and error estimates:
- Calculation of the effect concentration (EC50) after 24 and 48 hours was carried out by probit analysis (after the method according to LINDER und WEBER, confidence limit according to FIELLER). Due to the steep dose-response curve it was not possible to calculate confidence intervals for the 48 hours EC50.
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Under the conditions of this test the EC50 of CL-DAEP after 48 hours was 50.9 mg/L.
- Executive summary:
CL-DAEP was tested in Daphnia magna (water flea) for 48 hours in a static system. The concentrations tested were 22, 50, 100, 220 mg/L and a negative control (0 mg/L).
Before insertion of the test organisms the chamber contents were stirred for approxi-mately 24 hours with a magnetic-stirrer to ensure that the solubility limit of the test substance in the test water was reached.
All tested concentrations were visually present as clear solutions. No particulate matter was observed.
The substance concentration was determined in all tested groups. The values deter-mined were in a range of + 20 % of the theoretical value.
In this 48-hour acute toxicity study of CL-DAEP the following immobility values were determined:24h:
EC0 = 22.0 mg/L
EC50 = 69.9 mg/L (CI: 51.6 - 94.5 mg/L)
EC100 = > 100.0 mg/L
48h:
EC0 = 22.0 mg/L
EC50 = 50.9 mg/L
EC100 = 100.0 mg/L
In the control group no immobility was observed. Immobility was observed at 50, 100 and 220 mg/L. The 22 mg/L group showed no effect in comparison to the negative control.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.