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EC number: 680-413-6 | CAS number: 217437-44-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 02 - 04 Jul 2018
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 018
- Report date:
- 2018
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 13 Apr 2004
- Qualifier:
- according to guideline
- Guideline:
- other: OECD series 23 Guidance on aquatic toxicity testing of difficult substances and mixtures
- Version / remarks:
- 2000
- GLP compliance:
- yes
Test material
- Reference substance name:
- 2-[(3,5-dimethyl-1H-pyrazole-1-carbonyl)amino]ethyl 2-methylprop-2-enoate
- EC Number:
- 680-413-6
- Cas Number:
- 217437-44-0
- Molecular formula:
- C12H17N3O3
- IUPAC Name:
- 2-[(3,5-dimethyl-1H-pyrazole-1-carbonyl)amino]ethyl 2-methylprop-2-enoate
Constituent 1
Sampling and analysis
- Analytical monitoring:
- yes
- Remarks:
- UPLC
- Details on sampling:
- - Concentrations: All test concentrations and the control at test start (0 h) and test end (48 h).
- Sampling method: 1.2 mL were sampled from the approximate centre of the test vessels. At the end of the exposure period, the replicates were pooled at each concentrations before sampling. Reserve samples of 1.2 mL were additionally taken from all test solutions.
- Sample storage conditions before analysis: In a freezer (≤ - 15 °C) for a maximum of 3 months after delivery of the draft report.
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The preparation of test solutions started with a concentration of 100 mg/L test item in test medium and a 3-d period of magnetic stirring. The obtained mixture was allowed to settle for 3 h. Then, the aqueous saturated solution (SS) was collected by means of siphoning and used as the highest test concentration. Lower test concentrations were prepared by subsequent dilutions of the saturated solution in test medium.
- Controls: Test medium without test item
- Evidence of undissolved material: All test solutions were clear and colorless at the end of the preparation procedure.
- Other: The test item was a colourless to light yellow liquid and was not completely soluble in test medium at the loading rate initially prepared. No correction was made for the purity/composition of the test item.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Source: In-house laboratory culture with a known history
- Age at test start: < 24 h from parental daphnids of more than 2 weeks old. The daphnids originated from a healthy stock, 2nd to 5th brood, showing no signs of stress (i.e. > 20% mortality, presence of males, ephippia or discoloration, delay in 1st brood).
- Breeding conditions: Each batch was started with approximately 250 newborn daphnids, i.e. < 3 d old, which were placed in 5 L M7 medium (Elendt 1990) in an all-glass culture vessel. After 7 d of cultivation, half of the medium was renewed twice a week. Temperature: 18 - 22 °C.
- Feeding during breeding: Daily, a suspension of fresh water algae.
- Feeding during test: No
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
Test conditions
- Hardness:
- 180 mg/L CaCO3 (test medium)
- Test temperature:
- 19 - 20 °C
- pH:
- 7.7 - 8.1
- Dissolved oxygen:
- 8.5 - 9.0 mg/L
- Nominal and measured concentrations:
- Control and 100% saturated solution prepared at 100 mg/L (nominal, limit concentration)
1.0, 10% of the saturated solution (nominal, intermediate range-finding concentrations)
85 mg/L (48 h) and 89 mg/L (0 h) (measured in 100 mg/L solution) - Details on test conditions:
- TEST SYSTEM
- Test vessel: 60 mL all-glass test vessels filled with 50 mL test medium
- Aeration: No aeration of test solutions
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2 (low concentrations: range-finding test) and 4 (limit concentration: limit test)
- No. of vessels per control (replicates):4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The test medium was prepared by adding analytical grade salts to tap water purified by Reverse Osmosis (RO-water, GEON Waterbehandeling, Berkel-Enschot, The Netherlands)
- Culture medium different from test medium: Culture medium: M7 (Elendt, 1990); Test medium: Tap water and salts
- Intervals of water quality measurement: pH and dissolved oxygen were measured at the beginning and at the end of the test, for the highest test concentration and the control. The temperature of the medium was measured continuously in a temperature control vessel.
OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h dark
- Feeding: No feeding during test
EFFECT PARAMETERS MEASURED:
- Immobilization (including mortality): After 24 and 48 h
RANGE-FINDING STUDY
- Test concentrations: A combined limit/range-finding test was performed with nominal concentrations of 1.0, 10 and 100% of saturated solution prepared at a loading rate of 100 mg/L - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: No
- Mortality of control: 0% - Results with reference substance (positive control):
- - Results with reference substance valid?
Yes, the effect values are within the historical ranges.
- EC50 (48 h) = 0.59 mg/L, 95% confidence interval: 0.53 - 0.72 mg/L
- Other: The reference test was performed on 02 - 04 Jul 2018 according to OECD guideline 202. - Reported statistics and error estimates:
- No EC50 could be calculated because no effects were observed (EC50 > maximum concentration tested).
Any other information on results incl. tables
VALIDITY CRITERIA
The study fulfilled the criteria defined by the guideline (Table 1) and is thus considered valid and reliable.
Table 1: Validity criteria for OECD 202.
Criterion from the guideline |
Outcome |
Validity criterion fulfilled |
In the control, including the control containing the solubilising agent, not more than 10% of the daphnids should have been immobilized. |
In the control, no daphnids were immobilized or showed no other signs of disease or stress. |
Yes |
The dissolved oxygen concentration at the end of the test should be ≥ 3 mg/L in control and test vessels. |
The dissolved oxygen concentration at the end was ≥ 3 mg/L in control and test vessels. |
Yes |
ANALYTICAL RESULTS
The measured test item concentration in the undiluted saturated solution prepared at a nominal concentration of 100 mg/L was 89 mg/L at test start (0 h) and 85 mg/L at test end (48 h). Thus, the test item concentration was 96% of the initial concentration at test end (48 h) and thus remained stable for the duration of the test (Table 2). Therefore, it was concluded that the undissolved material removed during the preparation of the test samples was not test item related and the effect concentrations were based on the analytically confirmed nominal concentrations.
Table 2. Analytical results.
Sampling time |
Percentage of saturated solution1 |
Analyzed concentration |
Relative to initial |
[h] |
[%] |
[mg/L] |
[%] |
0 |
0 100 |
n.d. 89.1 |
|
48 |
0 100 |
n.d. 85.2 |
n.a. 96 |
1 Percentage of a saturated solution prepared at a loading rate of 100 mg/L
n.d. not detected
n.a. not applicable
BIOLOGICAL RESULTS
No immobility was observed in the control and at any of the test concentrations throughout the exposure period. The obtained effect values are summarized in Table 3.
Table 3. Summary of effect values.
Paramter |
Nominal test item concentration [mg/L] |
EC50 (24 h) and EC50 (48 h) |
> 100 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Remarks:
- For further details please refer to “Any other information on results incl. tables”.
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