Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 812-222-2 | CAS number: 4443-23-6
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2018-06-18 to 2018-10-16
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Municipal sewage treatment plant, 31137 Hildesheim, Germany
- Pretreatment: The activated sludge was washed twice with chlorine free tap water. After the second washing the settled sludge was resuspended in mineral salts medium and was maintained in an aerobic condition by aeration with CO2 free air until test start (2 days).
- Concentration of sludge: The amount of lnoculum used to initiate inoculation was 4.0 mL/L (25.0 mg/L dw).
- Initial cell/biomass concentration: 1E+07 to 1E+08 CFU/L; 1.36E+09/L at start - Duration of test (contact time):
- 28 d
- Initial conc.:
- 38 mg/L
- Based on:
- test mat.
- Remarks:
- Corresponding to an oxygen demand of 50.2 mg O2/L (69.2 mg O2/L with nitrification)
- Parameter followed for biodegradation estimation:
- O2 consumption
- Details on study design:
- TEST CONDITIONS
- Composition of medium: Mineral salts medium according to OECD 301 F
- Test temperature: Nominal: 20 - 24 ± 1 °C; Actual: 21.0 - 21.2 °C
- pH: 7.68 to 7.53
- pH adjusted: no
- Suspended solids concentration: 25 mg/L
- Continuous darkness: yes
TEST SYSTEM
- Number of culture flasks/concentration:
two for the lnoculum control (C1, C2)
one for the functional control (R1)
two for the test item (P1, P2)
one for the toxicity control (T1)
- Measuring equipment:
OxiTop® OC110 controller with software Achat OC, WTW
OxiTop® measuring heads, WTW
Stirring platform, WTW
Climatic exposure test cabinet, RUMED RUBARTH APPARATE GMBH
pH-Meter, Multi 350i, WTW
Datalogger 174 T, TESTO
Analytical balance, SARTORlUS
Balance, KERN
Magnetic stirrer, M83000, lab 4 you
Spectralphotometer NANOCOLORP UV/VlS, MACHEREY-NAGEL
Test Kits: Nitrat 50, Nitrit 2, all by MACHEREY-NAGEL
Multipette X-Stream, EPPENDORF
Various pipettes
- Test performed in open system: yes
- Details of trap for CO2 and volatile organics if used: A rubber sleeve with soda lime was hung into the opening of the bottles to absorb evolved CO2. The bottles were closed with OxiTop® measuring heads and the measuring system was activated.
SAMPLING
- Sampling frequency: 360 measuring points (every 112 minutes) during the 28 d incubation period.
CONTROL AND BLANK SYSTEM
- Inoculum blank: The inoculated test medium, consisting of the required volumes of mineral medium stock solutions, ultrapure water and lnoculum, was prepared in a measuring flask. 250 mL of this solution were filled in the brown glass bottles of the lnoculum control, using a
250 mL measuring flask.
- Functional control: The reference item was weighed out and transferred into a measuring flask, the required volumes of mineral medium stock solutions, ultrapure water and lnoculum, were added. 250 mL of this solution were filled in the brown glass bottle of the functional control, using a 250 mL measuring flask.
- Toxicity control: The reference item was weighed out and directly transferred into the test vessel with 250 mL test item medium (see above), using a 250 mL measuring flask.
STATISTICAL METHODS: - Reference substance:
- benzoic acid, sodium salt
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- Functional control
- Value:
- 10
- Sampling time:
- 1 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- Functional control
- Value:
- 93
- Sampling time:
- 10 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- Functional control
- Value:
- 100
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- Test item P1
- Value:
- 14
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- Test item P2
- Value:
- 17
- Sampling time:
- 28 d
- Key result
- Parameter:
- % degradation (O2 consumption)
- Remarks:
- Toxicity control
- Value:
- 40
- Sampling time:
- 28 d
- Details on results:
- Results of the Toxicity Control
ln the toxicity control the biodegradation achieved 38% after 14 days (Table 3, Figure 1), with 40% after 28 days. The validity criterion that the test item should not inhibit the biodegradation of the reference item was fulfilled.
Course and Stages of Biodegradation of Functional Control and Test item: Both test item replicates did not reach the 60% pass level within the 28-day-period of the study. The mean biodegradation on day 28 was 16%. ThOD NO3 of 69.2 mg O2/L (i.e. with nitrification) was considered, since the concentration of nitrate increased during the test. - Results with reference substance:
- Results of the Functional Control
The pass level for ready biodegradation (> 60% degradation) was reached within 3 days. The validity criterion that the degradation should be > 60% after 14 d was fulfilled. Stages of Biodegradation: The adaptation phase changed to the degradation phase within 1 day (degradation > 10%), with a maximum of 100% on day 15. - Validity criteria fulfilled:
- yes
- Interpretation of results:
- not readily biodegradable
- Conclusions:
- In the present study conducted according to OECD guideline 301F (1992) the biodegradability of the test item was measured in a Manometric Respirometry Test for 28 days. Biodegradation reached the 10% level on day 26 and a final biodegradation of 16% on day 28, thus the test item is not readily biodegradable under the conditions of the present test.
Reference
Nitrite and Nitrate Concentrations at Test End
|
Nitrite-N [mg/L] |
Nitrate-N [mg/L] |
corresponding oxygen consumption [mg/L] |
Biodegradation calculated without nitrification [%] |
Biodegradation calculated with nitrification [%] |
Test item P1 |
0 |
2.30 |
10.51 |
19 |
14 |
Test item P2 |
0 |
2.19 |
10.00 |
23 |
17 |
Description of key information
According to a OECD 301F (1992) test the test item reached the 10% level on day 26 and a final biodegradation of 16% on day 28, thus the test item is not readily biodegradable under the conditions of the present test.
Key value for chemical safety assessment
- Biodegradation in water:
- under test conditions no biodegradation observed
- Type of water:
- freshwater
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.