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EC number: 237-323-3 | CAS number: 13746-66-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 09-Aug-2019 - 18-Sep-2019
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Reason / purpose for cross-reference:
- reference to other study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Details on sampling:
- During the limit test samples for possible analysis were taken from the limit concentration and the control.
Frequency: at t=0 h and t=96 h
Volume: 4.8 mL
Storage: Samples were stored in a freezer (≤-15°C) until analysis at the analytical laboratory of the Test Facility.
Additionally, reserve samples of 4.8 mL were taken from all test solutions for possible analysis. If not already used, these samples were stored in a freezer (≤-15°C) for a maximum of three months after delivery of the draft report, pending on the decision of the sponsor for additional analysis. - Vehicle:
- no
- Details on test solutions:
- Preparation of test solutions started with the highest concentration of 100 mg/L. No other treatment than vigorous shaking was needed to completely dissolve the test item in medium. In the range-finding test, lower test concentrations were prepared by subsequent dilutions of the highest concentration in test medium. Coloration of test solutions increased with increasing test item concentrations from colorless to yellow. All test solutions were clear at the end of the preparation procedure.
- Test organisms (species):
- Cyprinus carpio
- Details on test organisms:
- TEST ORGANISM
- Common name: Carp (Cyprinus carpio, Teleostei, Cyprinidae) Linnaeus, 1758.
- Source: ‘De Haar Vissen’, Zodiac proefacc., Wageningen University and Research Centre, The Netherlands.
- Length at study initiation: Range-finding test: 2.8 ± 0.2 cm, Final test: 3.1 ± 0.2 cm.
- Weight at study initiation: Range-finding test: 0.21 ± 0.05 g, Final test: 0.29 ± 0.09 g.
- Characteristics: F1 from a single parent-pair bred in UV-treated water.
HOLDING
Quarantine/Acclimatisation: At least 12 days after delivery.
Holding Medium: The same medium as test medium.
Feeding: Daily with pelleted fish food (Essence 0.5-0.8 mm, Coppens International B.V., Helmond, The Netherlands).
Validity of batch: In the batch of fish used for the test, mortality during the seven days prior to the start of the test was less than 5%.
FEEDING DURING TEST
No feeding during the test period. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Hardness:
- 180 mg/L expressed as CaCO3
- Test temperature:
- 20 - 21 °C
- pH:
- 7.4 - 8.2
- Dissolved oxygen:
- 6.5 - 8.3
- Salinity:
- Not applicable
- Conductivity:
- Not applicable
- Nominal and measured concentrations:
- Nominal concentrations: 100 mg/L, the regulatory limit concentration.
Measured concentration: Measured concentrations were at the level of nominal throughout the test (i.e. 101 - 103% relative to the nominal concentration).
Based on these results, effect parameters were expressed based on analytically confirmed nominal concentrations.
It should be noted that a response was measured in the control samples at the start and at the end of the test. The origin of the detected response is unknown. Since the acceptability criteria were met, and no adverse effects were observed in the control or the limit
concentration, this response has no impact on the study results or conclusions. - Details on test conditions:
- TEST SYSTEM
- Test vessel: 10 litres, all-glass, containing 8 litres of test solution.
- Aeration: The test solutions were not aerated during the test.
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
- Loading rate: 0.25 g fish/litre, i.e. 7 fish per 8 litres of test medium.
TEST MEDIUM / WATER PARAMETERS :
- Test Medium: The following salts (analytical grade) were added to tap water purified by Reverse Osmosis (RO-water, GEON Waterbehandeling, Berkel-Enschot, The Netherlands):
CaCl2.2H2O: 211.5 mg/L
MgSO4.7H2O: 88.8 mg/L
NaHCO3: 46.7 mg/L
KCl: 4.2 mg/L
OTHER TEST CONDITIONS
- Adjustment of pH: no
- Photoperiod / intensity: A daily low light photoperiod of 16 hours at a light intensity of approximately 7 lux.
- Intervals of water quality measurement: Dissolved oxygen content, pH and temperature: Daily in all vessels with surviving fish, beginning at the start of the test (day 0).
EFFECT PARAMETERS MEASURED (96h-LC50) :
- Mortality and other effects: At 3, 24, 48, 72 and 96 hours following the start of exposure.
- In addition, every afternoon from day 0 and every morning from day 1 to observe for any dead or severely distressed fish.
TEST CONCENTRATIONS
- Spacing factor for test concentrations: a single concentration was tested (100 mg/L).
- Justification for using less concentrations than requested by guideline: Based on the results of the range-finding test.
- Range finding study
- Test concentrations: 0.1, 1.0, 1, 10 and 100 mg/L.
- Results used to determine the conditions for the definitive study: yes. - Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities: no behavioral abnormalities were observed in the control and the limit concentration throughout the test.
- Mortality of control: no - Results with reference substance (positive control):
- - Under the conditions of the present test, PCP induced no lethal effects in carp at 0.10 mg/L.
- The 96h-LC50 for carp exposed to PCP was 0.15 mg/L (95 % confidence interval between 0.10 and 0.22 mg/L).
- This effect was already reached within 24 hours of exposure. -
- Results with reference substance valid?: Yes - Reported statistics and error estimates:
- No LC50 could be calculated because the test item proved to be non-toxic (LC50 > maximum concentration).
- Validity criteria fulfilled:
- yes
- Conclusions:
- In conclusion, the 96h-LC50 for carp (Cyprinus carpio) exposed to Potassium Ferricyanide was beyond the range of concentrations tested, i.e. exceeded the analytically confirmed concentration of 100 mg/L.
- Executive summary:
In a 96-h acute toxicity study conducted according to OECD guideline 203 and GLP principles, carp (Cyprinus carpio) were exposed to Potassium Ferricyanide under static conditions at the following nominal concentrations in a limit test: 0 (blank control) and 100 mg/L. The measured concentrations were at the level of nominal throughout the test (i.e. 101 - 103% relative to the nominal concentration). Based on these results, effect parameters were expressed based on analytically confirmed nominal concentration. The test substance did not induce visible or lethal effects in carp at 100 mg/L. The 96h-LC50 was >100 mg/L. The study is considered to be reliable without restrictions.
Reference
Description of key information
The 96-h LC50 for acute toxicity to fish was determined to be >100 mg/L (analytically confirmed nominal exposure concentration).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 100 mg/L
Additional information
In a 96-h acute toxicity study conducted according to OECD guideline 203 and GLP principles, carp (Cyprinus carpio) were exposed to Potassium Ferricyanide under static conditions at the following nominal concentrations in a limit test: 0 (blank control) and 100 mg/L. The measured concentrations were at the level of nominal throughout the test (i.e. 101 - 103% relative to the nominal concentration). Based on these results, effect parameters were expressed based on analytically confirmed nominal concentration. The testsubstance did not induce visible or lethal effects in carp at 100 mg/L. The 96h-LC50 was >100 mg/L. The study is considered to be reliable without restrictions.
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