N,N'-[(methylimino)bis(trimethylene)]bis(stearamide)

Solubility in organic solvents / fat solubility

Link to relevant study record(s)

Reference

Endpoint:

solubility in organic solvents / fat solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2016-03-31 to 2016-04-15

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

other: OECD Guideline 105 (Water Solubility)

Version / remarks:

1995-07-27

Deviations:

no

GLP compliance:

no

Key result

Medium:

n-octanol

Solubility:

5.972 g/L

Temp.:

20 °C

PRELIMINARY TEST

Different amounts of the test item were suspended in n-octanol and the respective mixture was stirred at room temperature for 24 h.

The cloud point of the test item is about 0.01 g/2 mL. A 5-fold concentration of the test item was used for the investigation.

CONDUCT OF THE STUDY

About 500 mg of the test item were suspended in 20 ml n-octanol and stirred in a heated water bath at 30 °C for 24 h, 48 h, and 72 h, respectively.

Time Interval	Mass Test Item
24 h	501.39 mg
48 h	500.24 mg
72 h	500.88 mg

After incubation, the sarnple suspensions were cooled to 20 °C and kept at this temperature for 24 h without stirring. Afterwards the samples were centrifuged (3500 U/min) at 20 °C for 30 minutes. Above the sediments of all samples clear liquids were obtained. The clear liquids of the three test solutions (24 h, 48 h and 72h) were isolated, diluted 2.0 ml/50 mL tetrahydrofuran and analysed by liquid chromatography.

STUDY RESULTS

The concentrations of the different test solutionswere calculated automatically using the equation of the calibration curve. The results are summarised in the table below:

Test mixture	Time [h]	Concentration of test item [mg/50 mL]	Average of test item [mg/50 mL]
1	24	11.843	11.88
		11.912
2	48	11.865	12.00
		12.140
3	72	11.888	11.95

These analytical results allow the calculation of the n-octanol solubility of the test item.

Test mixture	Time [h]	Average of test item [g/L]	Total average of test item [g/L]
1	24	5.940	5.972 ± 0.0301
2	48	6.000
3	72	5.975

n-Octanol solubility of the test item is 5.972 g/L ± 0.0301 g/L.

It was integrated over all peaks (retention time 20 -38 min) and the external standard method was used. Additionally an accumulation of the peaks at retention times from 21 min to 26 min is observed (cp. overlay of the chromatograms).

The n-octanol solubility was determined at 20 °C.

Conclusions:

The n-Octanol solubility of N,N'-[(methylimino)bis(trimethylene)]bis(stearamide) was determined to be 5.972 g/L ± 0.0301 g/L.

Executive summary:

The solubility of N,N'-[(methylimino)bis(trimethylene)]bis(stearamide) in n-Octanol was measured according to OECD Guideline 105 (27 July 1995) using the flask method at 20 °C.

It was integrated over all peaks (retention time: 20 —38 min) and the extemal standard method was used. Additionally an accumulation of the peaks at retention times from 21 min to 26 min is observed (cp. overlay of the chromatograms).

The n-Octanol solubility of N,N'-[(methylimino)bis(trimethylene)]bis(stearamide) was determined to be 5.972 g/L ± 0.0301 g/L.

Description of key information

The n-Octanol solubility of N,N'-[(methylimino)bis(trimethylene)]bis(stearamide) was determined to be 5.972 g/L ± 0.0301 g/L.

Key value for chemical safety assessment

Solubility in organic solvents at 20°C:

5.972 g/L