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EC number: 218-254-8 | CAS number: 2094-98-6
The study was performed using 5 concentrations ranging from 1 to 100 mg/L (nominal). Incubation time (test system Desmodesmus subspicatus)was 72 hours. The cell concentration of each replicate was determined by measuring the cell numbers every 24 hours with an electronic particle counter. Growth rate µ and the yield were determined from the cell number at the respective observation times.
Significant inhibition of algal growth was observed at the following concentrations:
3.2 – 100 mg/L (nominal concentrations)
At the beginning and at the end of the test, the content of the test item in the test solutions was determined using HPLC-UV-determination. Because of the poor solubility of the test item the measured concentrations lay between 1 % and 4 % of the nominal concentration during the test. Therefore, the determination of the results was based on the geometric mean of the measured concentrations.
The 72h-EC50values of potassium dichromate (K2Cr2O7, CAS No. 7778-50-9) were determined in a separate reference test. The values lay within the range of the laboratory (growth rate 0.73 - 1.10 mg/L, yield 0.21 – 0.66 mg/L).
The following results for the test item 1,1'-Azobis(cyclohexane-1-carbonitrile) were determined:
Table 3‑a Results of the test item
> 1.85 mg/L
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