[(8R,9S,10R,13S,14S,17R)-3-ethoxy-17-ethynyl-13-methyl-2,7,8,9,10,11,12,14,15,16-decahydro-1H-cyclopenta[a]phenanthren-17-yl] acetate

Surface tension

Administrative data

Endpoint:

surface tension

Data waiving:

study scientifically not necessary / other information available

Justification for data waiving:

the study does not need to be conducted because surface activity is not a desired property of the material

the study does not need to be conducted because water solubility is below 1 mg/L at 20°C

Justification for type of information:

JUSTIFICATION FOR DATA WAIVING
The water solubility of the test item is 0.0106 mg/L (see endpoint 4.8).
According to the guidelines, substances with a water solubility < 1 mg/L need not to be tested. The test on the surface tension of the test item was therefore not required.

Cross-reference

Reason / purpose for cross-reference:

data waiving: supporting information

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

17 september 2018 - 08 November 2018

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Version / remarks:

2016

Deviations:

no

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7840 (Water Solubility)

Version / remarks:

1998

Deviations:

no

GLP compliance:

yes

Type of method:

column elution method

Key result

Water solubility:

0.001 mg/L

Conc. based on:

test mat.

Temp.:

20 °C

pH:

>= 7.4 - <= 7.6

Remarks on result:

other: average of two flow rates

Details on results:

Mean concentration in the water samples (n=10) obtained at the flowrate of 24 mL/h was 0.00036 mg/L. CV = 19%.
Mean concentration in the water samples (n=10) obtained at the flowrate of 12 mL/h was 0.00058 mg/L. CV = 16%.
Mean concentration in the water samples (n=10) obtained at the flowrate of 6 mL/h was 0.00052 mg/L. CV = 23%.
The maximum difference on the mean values at flow rates 24 and 12 mL/h was > 30% (i.e. 47%). The maximum difference on the mean values at flow rates 12 and 6 mL/h was ≤ 30% (i.e. 11%). Therefore the water solubility of the test item is based on the results of the 12 and 6 mL/h samples and is reported as 0.00055 mg/L.

The turbidity of the eluates was < 0.2 NTU. According to this, no undissolved particles were detected in the eluates.

No test item was detected in the samples from the blank column.

Conclusions:

The water solubility of the substance at 20°C was determined to be 0.00055 mg/L.

Executive summary:

The water solubility of the substance was determined using the column elution method in a GLP study according to EC A.6, OECD 105 and OPPTS 830.7840.

Quantification was performed by UPLC-MS/MS. The water solubility of the substance at 20°C was determined to be 0.00055 mg/L (average of two flow rates: 12 mL/h and 6 mL/h).

The coefficient of variation (n=10) was ≤ 30% at both flow rates. The maximum difference on the mean values at both flow rates was ≤ 30%.

The pH of the aqueous samples was 7.6 and 7.4.