Registration Dossier
Registration Dossier
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EC number: 272-062-9 | CAS number: 68683-16-9
Endpoint summary
Administrative data
Description of key information
waiving
Additional information
The test substance is highly insoluble in water therefore studies on aquatic toxicity can be waived according to the REACH Regulation N. 1907 -2006 .
Data on range finding tests carried out both on daphnia and alga species are however available.
Range finding on Daphnia
A non-GLP preliminary range finding tests under semi-static conditions over a period of 48 hours for Daphnia was conducted with a saturated solution of the test item prepared at a loading rate of 100 mg/L and three further dilution levels prepared by dilution of the saturated solution by factor 10, 100 and 1000 with dilution water.
The saturated solution was prepared four days prior to the start of the exposure (at day -4). No Tyndall effect was observed in the saturated solution at the start of the exposure (0 hours) and at renewal of the test solutions (24 hours).
In the range finding test, two replicates per concentration level and control, each with five daphnids, were tested. The preliminary range finding test was conducted under diffuse light conditions (light intensity of max. 1500 lx, 16/8 hours light/dark cycle).
The measured concentrations of chromium were below the limit of detection (0.005 mg Cr/L) in all samples.
Under this experimental condition no effects were observed on Daphnia.
Range finding on Alga
A non-GLP preliminary range finding test under static conditions over a period of 72 hours was conducted. A saturated solution with a nominal loading of 100 mg test item/L was freshly prepared. Out of this saturated solution three concentration levels were prepared (by serial dilution) with the concentrations 0.100, 1.00 and 10.0% of the saturated solution.
The concentrations of the test item were analytically verified via photometer at the start and at the end of the exposure. The measured concentrations were below the lowest calibration level (0.05 mg/L) in all test concentrations.
All concentration levels were clear throughout the exposure period.
Under this experimental considitions no effects on alga were observed.
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