Registration Dossier

Environmental fate & pathways

Biodegradation in water: screening tests

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
From August 9, 2017 to September 28, 2017
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to
Guideline:
OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: Dihua sewage treatment plant, Taiwan.
Duration of test (contact time):
29 d
Initial conc.:
20 mg/L
Based on:
TOC
Reference substance:
acetic acid, sodium salt
Remarks:
Sodium acetate
Parameter:
% degradation (CO2 evolution)
Value:
< 60
Sampling time:
29 d
Results with reference substance:
At Day 14 during the study, the biodegradation was 72.68 %.

Table 1. The CO2 production of blank control group, test group, reference control group and toxicity control group

Time

(Day)

Blank control group (mg)

Test group (mg)

Reference control group (mg)

Toxicity control group (mg)

(1)

(2)

Mean

(1)

(2)

2

3.41

2.75

3.08

3.63

4.18

20.46

19.80

5

3.85

1.21

2.53

2.31

2.53

23.21

23.76

7

1.54

0.88

1.21

2.09

0.88

14.30

14.85

9

3.74

3.52

3.63

2.75

3.30

10.56

9.68

14

4.51

4.62

4.57

4.84

4.62

10.45

9.79

19

3.08

2.97

3.03

3.30

3.30

7.15

7.81

23

4.84

4.95

4.90

3.96

5.17

5.50

6.60

27

7.70

6.82

7.26

6.05

7.59

9.57

8.91

29

5.39

5.17

5.28

4.18

4.95

7.26

6.16

Table 2. The cumulative CO2 production and percentage biodegradation of blank control group, test group, reference control and toxicity control group

Time

(Day)

Cumulative CO2 production (mg)

Percentage biodegradation (% ThCO2)

Blank control group

Test group

Reference control group

Toxicity control group

Test group

Reference control group

Toxicity control group

Mean

(1)

(2)

(1)

(2)

Mean

2

3.08

0.55

1.10

17.38

16.72

0.62

1.25

0.94

19.75

11.39

5

5.61

0.33

1.10

38.06

37.95

0.37

1.25

0.81

43.24

25.85

7

6.82

1.21

0.77

51.15

51.59

1.37

0.87

1.12

58.12

35.14

9

10.45

0.33

0.44

58.08

57.64

0.37

0.50

0.44

65.99

39.26

14

15.02

0.61

0.49

63.97

62.87

0.69

0.56

0.62

72.68

42.82

19

18.04

0.88

0.77

68.09

67.65

1.00

0.87

0.94

77.37

46.08

23

22.94

-0.06

1.04

68.70

69.36

-0.06

1.19

0.56

78.05

47.24

27

30.20

-1.27

1.37

71.01

71.01

-1.44

1.56

0.06

80.68

48.37

29

35.48

-2.37

1.04

72.99

71.89

-2.69

1.19

-0.75

82.93

48.97

Validity criteria fulfilled:
yes
Interpretation of results:
not readily biodegradable
Conclusions:
According to OECD 301B test method, CR SB87 was not be considered readily biodegradable.
Executive summary:

This test using the procedures outlined in the SuperLab Study Plan for M62-170700011001EN which is based on the SOP for the OECD 301B (SOPE-503) and OECD 301B (OECD, 1992). The results of this OECD 301B test for CR SB87 show that all validity criteria were met. CR SB87 can not be considered readily biodegradable under the test conditions and the percentage biodegradation of CR SB87 was less than 60 %.

Description of key information

Not readily biodegradable (OECD TG 301B).

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

The total amount of evolved CO2 in the blank control group was 35.48 mg/1.2 L. The difference between replicates of test group was less than 20%. At Day 14 during the study, the biodegradation of the reference control article was 72.68 % and the percent biodegradation in the toxicity control was 42.82 %. All test validity criteria were met. CR SB87 can not be considered readily biodegradable under the test conditions and the percentage biodegradation of CR SB87 was less than 60 %.