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Environmental fate & pathways

Hydrolysis

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Administrative data

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Reference
Endpoint:
hydrolysis
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Guideline study with GLP.
Qualifier:
according to guideline
Guideline:
EU Method C.7 (Degradation: Abiotic Degradation: Hydrolysis as a Function of pH)
Deviations:
no
GLP compliance:
yes
Radiolabelling:
no
Analytical monitoring:
yes
Details on sampling:
According to the EC-method.
Buffers:
Buffer pH 4: 250 mL 0.1 mol/L potassium dihydrogencitrate and 45 mL 0.1 mol/L sodium hydroxide filled up to 500 mL with water.
Buffer pH 7: 250 mL 0.1 mol/L potassium dihydrogenphosphate and 148.2 mL 0.1 mol/L sodium hydroxide filled up to 500 mL with water.
Buffer pH 9: 250 mL 0.1 mol/L boric acid in 0.1 mol/L potassium chloride and 106.5 mL 0.1 mol/L sodium hydroxide filled up to 500 mL with water.
The water (for analysis) was autoclaved before use.
Estimation method (if used):
The water solubility of the test substance is 0.08 mg/L at 20 °C. A stock suspension of the test substance was prepared by dissolving 10.6 mg of the test substance in 250 mL autoclaved water (for analysis). The stock suspension was placed in a ultrasonic bath for 30 minutes. Afterwards the suspension was filtered trough a 0.2 µm filter. For each pH, 50 mL of the stock solution was diluted with 50 mL of the appropriate buffer. Afterwards nitrogen was bubbled through each solution to expel oxygen. The concentrations of the resulting solutions were in the range of the half of the saturation concentration. The solutions were kept in a water bath at the test temperature in the dark.
Portions of the solutions were taken from the flasks and the concentration of the test substance was analysed by HPLC.
Temperatures were recorded several times during the incubation.
The pH of each solution was determined at each sampling time with a pH meter.
Details on test conditions:
No main test was performed as the hydrolysis rate in the preliminary test was high and an estimation of the half life at 25 °C was possible.
Duration:
0.47 h
pH:
3.98
Temp.:
50 °C
Initial conc. measured:
0 g/L
Duration:
0.36 h
pH:
6.99
Temp.:
50 °C
Initial conc. measured:
0 g/L
Duration:
0.33 h
pH:
8.98
Temp.:
50 °C
Initial conc. measured:
0 g/L
Number of replicates:
1
Positive controls:
no
Negative controls:
no
Statistical methods:
Not applicable.
Preliminary study:
More than 50 % of the test substance at pH 4, 7 and 9 hydrolysed in 2.4 hours at 50 °C. According to the EU method it is estimated that the half-life of T15-AE in aqueous solution at 25 °C is less than one day.
Transformation products:
not measured
Details on hydrolysis and appearance of transformation product(s):
More than 50 % of the test substance at pH 4, 7 and 9 hydrolysed in 2.4 hours at 50 °C. According to the EU method it is estimated that the half-life of T15-AE in aqueous solution at 25 °C is less than one day. No main test was therefore performed.
pH:
4
Temp.:
50 °C
Hydrolysis rate constant:
3 h-1
DT50:
0.23 h
Type:
(pseudo-)first order (= half-life)
Remarks on result:
other: Pseudo-first order was assumed.
pH:
4
Temp.:
25 °C
DT50:
< 24 h
Remarks on result:
other: According to EU-method.
pH:
7
Temp.:
50 °C
Hydrolysis rate constant:
3.3 h-1
DT50:
0.21 h
Type:
(pseudo-)first order (= half-life)
Remarks on result:
other: Pseudo-first order was assumed.
pH:
7
Temp.:
25 °C
DT50:
< 24 h
Remarks on result:
other: According to the EU-method.
pH:
9
Temp.:
50 °C
Hydrolysis rate constant:
> 6.2 h-1
DT50:
< 0.11 h
Type:
(pseudo-)first order (= half-life)
Remarks on result:
other: Pseudo-first order was assumed.
pH:
9
Temp.:
25 °C
DT50:
< 24 h
Remarks on result:
other: According to the EU-method.
Validity criteria fulfilled:
not applicable
Conclusions:
The half-life of T15-AE in aqueous solution at 25 °C at pH 4, 7 and 9 is less than one day
Executive summary:

The rate of hydrolysis of the substance was determined as a function of pH, according to EU method C.7. The rate constants at pH 4, pH 7 and pH 9 were determined at 50 °C.

Results:

At pH4, 50 °C: rate constant is 3.0 h-1; half life is 0.23 h

At pH7, 50 °C: rate constant is 3.3 h-1; half life is 0.21 h

At pH9, 50 °C: rate constant is >6.2 h-1; half life is <0.11 h

More than 50 % of the test substance at pH 4, 7 and 9 hydrolysed in 2.4 hours at 50 °C. According to the EU method it is estimated that the half-life of T15-AE in aqueous solution at 25 °C is less than one day.

Description of key information

The rate constants at pH 4, pH 7 and pH 9 were determined at 50 °C.
Results:
At pH4, 50 °C: rate constant is 3.0 h-1; half life is 0.23 h
At pH7, 50 °C: rate constant is 3.3 h-1; half life is 0.21 h
At pH9, 50 °C: rate constant is >6.2 h-1; half life is <0.11 h
More than 50 % of the test substance at pH 4, 7 and 9 hydrolysed in 2.4 hours at 50 °C.

According to the EU method it is estimated that the half-life of T15-AE in aqueous solution at 25 °C is less than one day.

Key value for chemical safety assessment

Half-life for hydrolysis:
0.21 h
at the temperature of:
50 °C

Additional information