sodium hydrogen [5-(diethylamino)-2-[(2-hydroxy-4-nitrophenyl)azo]phenolato(2-)][5-hydroxy-6-[(2-hydroxy-4-nitrophenyl)azo]-1-naphthalenesulfonato(3-)]-, chromate(2-)

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: screening test, other

Remarks:

BOD5/COD

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

From June 6 to 7, 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Remarks:

The test was conducted by means of Read Across approach. The reliability of the source study report is 1. Further information was attached at section 13

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

other: DEV DIN 38*409 - H 41-1 (1980)

Qualifier:

equivalent or similar to guideline

Guideline:

EU Method C.6 (Degradation: Chemical Oxygen Demand)

Deviations:

yes

Remarks:

Mean of 2 determinations instead of 3

Principles of method if other than guideline:

Internal Method: SAV 02 000 02

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

not specified

Duration of test (contact time):

ca. 2 h

Initial conc.:

ca. 10 other: mg

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 148 ± 3 °C

Reference substance:

other: Potassium hydrogen phthalate

Parameter:

% degradation (O2 consumption)

Remarks on result:

not determinable

Parameter:

COD

Value:

ca. 496 mg O2/g test mat.

Parameter:

BOD5*100/COD

Value:

ca. 0.8

Remarks on result:

other: 0.8 is a dimensionless value

Results with reference substance:

COD = 198 mg O2/l

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

COD = 496 mg O2/g
BOD5/COD = 0.008

Executive summary:

Methods:

The Chemical Oxygen Demand of test substance was determined according to the DEV DIN 38*409 - H 41-1 (1980), similar to EU Method C.6 (Degradation: Chemical Oxygen Demand).

Results:

COD = 496 mg O2/g

BOD5/COD = 0.008

Reference 2

Endpoint:

biodegradation in water: inherent biodegradability

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

From April 20 to May 18, 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study

Remarks:

The test was conducted by means of Read Across approach. The reliability of the source study report is 1. Further information was attached at section 13

Qualifier:

according to guideline

Guideline:

OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: EEC-Directive, L133 (pp. 99-105) Volume 31, May 88.

Deviations:

no

Principles of method if other than guideline:

Internal method: SAV:13 000 02

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

other: Sludge of a biological sewage treatment plant

Details on inoculum:

- Source of inoculum: Sludge of a biological sewage treatment plant
- Laboratory culture: (ARA-Reinach) collected on 19.4.94
- Initial cell/biomass concentration: 930 mg/l

Duration of test (contact time):

ca. 28 d

Initial conc.:

ca. 150 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Details on study design:

TEST CONDITIONS
- Water / Nutrient: according to the specification of the EEC L133 (pp. 99-105) Volume 31 resp. OECD Method 302B.
- Test temperature: 22.0 ± 3°C
- Lighting: indirect daylight
- Estimation Technique: TOC/DOC Analyzer/ SHIMADZU TOC-5000

Reference substance:

diethylene glycol

Parameter:

% degradation (DOC removal)

Value:

ca. 22.9

Sampling time:

28 d

Parameter:

% degradation (DOC removal)

Value:

ca. 25.3

Sampling time:

3 h

Remarks on result:

other: adsorption

Results with reference substance:

bioelimination of the reference substance after 14 days is : 99.7 %

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

Bioelimination of test substance measured as DOC after 28 days corrected by the blank control is: 22.9 %
Adsorption after 3 hours: 25.3 %

Executive summary:

Methods:

The inherent biodegradability of test substance was determined in a 28 days Zahn-Wellens -test according to the OECD Guideline for Testing of Chemicals, No. 302B, 1981.

Results:

Bioelimination of test substance measured as DOC after 28 days corrected by the blank control is: 22.9%.

Adsorption after 3 hours: 25.3%

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Remarks:

BOD5/COD

Type of information:

other: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Study period:

From June 8 to 14, 1994

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Remarks:

The test was conducted by means of Read Across approach. The reliability of the source study report is 1. Further information was attached at section 13

Reason / purpose for cross-reference:

reference to other study

Qualifier:

according to guideline

Guideline:

ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method)

Deviations:

yes

Remarks:

Aerate over night after addition of the inoculum

Principles of method if other than guideline:

Internal Method: SAV 01 000 03

GLP compliance:

yes

Oxygen conditions:

aerobic

Inoculum or test system:

other: Seeding water of an urban waste water

Duration of test (contact time):

ca. 5 d

Initial conc.:

ca. 6 mg/L

Based on:

test mat.

Initial conc.:

ca. 13 mg/L

Based on:

test mat.

Initial conc.:

ca. 26 mg/L

Based on:

test mat.

Initial conc.:

ca. 51.9 mg/L

Based on:

test mat.

Initial conc.:

ca. 103.9 mg/L

Based on:

test mat.

Initial conc.:

ca. 207.7 mg/L

Based on:

test mat.

Initial conc.:

ca. 415.5 mg/L

Based on:

test mat.

Initial conc.:

ca. 830.9 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Details on study design:

TEST CONDITIONS
- Test temperature: 20 ± 1 °C

Reference substance:

other: D(+) - Glucose / L-Glutamic acid

Parameter:

% degradation (O2 consumption)

Remarks on result:

not determinable

Parameter:

BOD5

Value:

ca. 4 mg O2/g test mat.

Parameter:

BOD5*100/COD

Value:

ca. 0.8

Remarks on result:

other: dimensionless value

Results with reference substance:

207 mg O2/l

Validity criteria fulfilled:

yes

Interpretation of results:

not readily biodegradable

Conclusions:

BOD5 = 4 mg O2/g
BOD5/COD= 0.008

Executive summary:

Methods:

The Biological Oxygen Demand of test substance was determined according to the ISO 5815 (Water quality - Determination of Biochemical Oxygen Demand after 5 Days (BOD5) - Dilution and Seeding Method).

Results:

BOD5 = 4 mg O₂/g

BOD5/COD= 0.008

Description of key information

DOC removal (28 d) = 22.9%; adsorption after 3h = 25.3%.

BOD5/COD= 0.008  

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable