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EC number: 268-211-2 | CAS number: 68037-36-5
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 22 October 2017 to 30 October 2017
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- other: ASTM Standard E729-96
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- not specified
- Details on sampling:
- - Concentrations: single WAF loading rate of 4.0 mg active ingredient/L and a negative control (dilution water).
- Vehicle:
- no
- Details on test solutions:
- A single water accommodated fraction (WAF) was prepared for the 4.0 mg a.i./L loading rate in a 13.2 L Pyrex™ aspirator bottle with tubulation and spigot approximately 2-3 cm from the bottom. Dilution water was measured (12 L) and added into the labeled WAF bottle. 0.0480 grams of the test material was weighed into tared glass beaker. The test material was rinsed with a portion of the dilution water into the appropriate WAF bottle and stirred on a magnetic stir plate for 48 hours with a Teflon-lined stir bar, with a vortex depth of approximately 30% of the test solution height. The negative control solution was prepared in exactly the same manner as the test solution was prepared, but with dilution water only and no test substance. At the beginning of the stirring process, the negative control appeared clear and colorless while the 4.0 mg a.i./L solution appeared clear and blue with blue precipitate on the walls of the WAF bottle just above the water surface. At the termination of stirring process, the negative control appeared clear and colorless and the 4.0 mg a.i./L test solution appeared clear and blue with blue precipitate on the water surface and at the bottom of the WAF bottle. The solutions were allowed to settle for approximately 4 hours to allow phase separation before decanting (~11 L each) the aqueous phase. The solutions were filtered into each test chamber through glass wool. At the end of the settling period, the negative control appeared clear and colorless and the 4.0 mg a.i./L solution appeared clear and blue with blue precipitate on the walls of the test chamber on the water surface and at the bottom of the WAF bottle.
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- TEST ORGANISM
- Common name: Fathead minnow
- Strain: Pimephales promelas
- Source: Osage Catfisheries Inc., Missouri.
- Age at study initiation (mean and range, SD): Juvenile
- Length at study initiation (length definition, mean, range and SD): The average total length of 7 negative control fish measured at the end of the test was 3.1 cm, with a range of 2.5 to 3.3 cm.
- Weight at study initiation (mean and range, SD): The average wet weight (blotted dry) of 7 negative control fish measured at the end of the test was 0.25 grams, with a range of 0.11 to 0.30 grams.
- Method of breeding: Not applicable
- Maintenance of the brood fish: Not reported
ACCLIMATION
- Acclimation period: at least 14 days prior to the test
- Acclimation conditions (same as test or not): same as test conditions; water temperatures in the cultures ranged from 20.9 to 23.3 degrees Celcius, the pH of the water ranged from 8.3 to 8.6, dissolved oxygen concentrations were ≥8.3 mg/L (≥95% of saturation).
- Type and amount of food during acclimation: brine shrimp nauplii (Artemia sp.).
- Feeding frequency during acclimation: The fish were not fed for two days prior to the test or during the test.
- Health during acclimation (any mortality observed): During this 2-week period, the fish in the lot used for the test showed no signs of disease or stress and there were no mortalities.
FEEDING DURING TEST :
- Frequency: The fish were not fed for two days prior to the test or during the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Observations were made at 24, 48, 72 and 96 hours.
- Hardness:
- 141 mg/L as CaCO3
- Test temperature:
- 22 ± 1 degrees Celcius
- pH:
- 8.1
- Dissolved oxygen:
- ≥8.3 mg/L (≥95% of saturation)
- Salinity:
- Not applicable
- Conductivity:
- Specific Conductance: 344 µS/cm
- Nominal and measured concentrations:
- A single WAF loading rate of 4.0 mg active ingredient/L.
- Details on test conditions:
- TEST SYSTEM
- Test vessel: 25-L stainless steel aquaria
- Material, size, headspace, fill volume: The depth of the test water in a representative chamber was 11.9 cm.
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1 test chamber per concentration
- No. of vessels per control (replicates): 1 test chamber per concentration
- Biomass loading rate: 0.1572 g/L
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Freshwater obtained from a well approximately 40 meters deep located on the EAG Laboratories-Easton site.
- Total organic carbon: <2 mg C/L
- Particulate matter: The well water was passed through a sand filter to remove particles greater than approximately 25 microns, and pumped into a 37,800-L storage tank where the water was aerated with spray nozzles. Prior to use, the water was filtered to 0.45 microns to remove fine particles
- Pesticides: none, UV-sterilized
- Alkalinity: 179 mg/L as CaCO3 - Key result
- Duration:
- 96 h
- Dose descriptor:
- LL50
- Effect conc.:
- > 4 other: a.i./L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: no mortality
- Key result
- Duration:
- 96 h
- Dose descriptor:
- other: NOEL
- Effect conc.:
- 4 other: a.i./L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: No mortality
- Key result
- Duration:
- 96 h
- Dose descriptor:
- other: Maximum WAF Loading Rate Causing 0% Mortality
- Effect conc.:
- 4 other: a.i./L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: No mortality
- Key result
- Duration:
- 96 h
- Dose descriptor:
- other: Minimum WAF Loading Rate Causing 100% Mortality
- Effect conc.:
- > 4 other: a.i./L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: No mortality
- Details on results:
- Not reported
- Results with reference substance (positive control):
- Not applicable
- Validity criteria fulfilled:
- yes
- Conclusions:
- A 96-Hour Static Acute Toxicity Test with the Fathead Minnow (Pimephales promelas) exposed to WAF loading rate of 4.0 mg a.i./L., performed according to OECD guideline 203; U.S. EPA method 850.1075, and ASTM Standard E729-96. Based on nominal water accommodated fraction (WAF) loading rate: 96-Hour LL50: >4.0 mg a.i./L No-Observed-Effect Level: 4.0 mg a.i./L Maximum WAF Loading Rate Causing 0% Mortality 4.0 mg a.i./L Minimum WAF Loading Rate Causing 100% Mortality >4.0 mg a.i./L.
- Executive summary:
A 96-Hour Static Acute Toxicity Test with the Fathead Minnow (Pimephales promelas) exposed to WAF loading rate of 4.0 mg a.i./L., performed according to OECD guideline 203; U.S. EPA method 850.1075, and ASTM Standard E729-96. Based on nominal water accommodated fraction (WAF) loading rate: 96-Hour LL50: >4.0 mg a.i./L No-Observed-Effect Level: 4.0 mg a.i./L Maximum WAF Loading Rate Causing 0% Mortality 4.0 mg a.i./L Minimum WAF Loading Rate Causing 100% Mortality >4.0 mg a.i./L.
Reference
Description of key information
A 96-Hour Static Acute Toxicity Test with the Fathead Minnow (Pimephales promelas) exposed to WAF loading rate of 4.0 mg a.i./L., performed according to OECD guideline 203; U.S. EPA method 850.1075, and ASTM Standard E729-96. Based on nominal water accommodated fraction (WAF) loading rate: 96-Hour LL50: >4.0 mg a.i./L No-Observed-Effect Level: 4.0 mg a.i./L Maximum WAF Loading Rate Causing 0% Mortality 4.0 mg a.i./L Minimum WAF Loading Rate Causing 100% Mortality >4.0 mg a.i./L.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 4 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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