Copper, [29H,31H-phthalocyaninato(2-)-N29,N30,N31,N32]-, sulfonated

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

22 October 2017 to 30 October 2017

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

other: ASTM Standard E729-96

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

not specified

Details on sampling:

- Concentrations: single WAF loading rate of 4.0 mg active ingredient/L and a negative control (dilution water).

Vehicle:

no

Details on test solutions:

A single water accommodated fraction (WAF) was prepared for the 4.0 mg a.i./L loading rate in a 13.2 L Pyrex™ aspirator bottle with tubulation and spigot approximately 2-3 cm from the bottom. Dilution water was measured (12 L) and added into the labeled WAF bottle. 0.0480 grams of the test material was weighed into tared glass beaker. The test material was rinsed with a portion of the dilution water into the appropriate WAF bottle and stirred on a magnetic stir plate for 48 hours with a Teflon-lined stir bar, with a vortex depth of approximately 30% of the test solution height. The negative control solution was prepared in exactly the same manner as the test solution was prepared, but with dilution water only and no test substance. At the beginning of the stirring process, the negative control appeared clear and colorless while the 4.0 mg a.i./L solution appeared clear and blue with blue precipitate on the walls of the WAF bottle just above the water surface. At the termination of stirring process, the negative control appeared clear and colorless and the 4.0 mg a.i./L test solution appeared clear and blue with blue precipitate on the water surface and at the bottom of the WAF bottle. The solutions were allowed to settle for approximately 4 hours to allow phase separation before decanting (~11 L each) the aqueous phase. The solutions were filtered into each test chamber through glass wool. At the end of the settling period, the negative control appeared clear and colorless and the 4.0 mg a.i./L solution appeared clear and blue with blue precipitate on the walls of the test chamber on the water surface and at the bottom of the WAF bottle.

Test organisms (species):

Pimephales promelas

Details on test organisms:

TEST ORGANISM
- Common name: Fathead minnow
- Strain: Pimephales promelas
- Source: Osage Catfisheries Inc., Missouri.
- Age at study initiation (mean and range, SD): Juvenile
- Length at study initiation (length definition, mean, range and SD): The average total length of 7 negative control fish measured at the end of the test was 3.1 cm, with a range of 2.5 to 3.3 cm.
- Weight at study initiation (mean and range, SD): The average wet weight (blotted dry) of 7 negative control fish measured at the end of the test was 0.25 grams, with a range of 0.11 to 0.30 grams.
- Method of breeding: Not applicable
- Maintenance of the brood fish: Not reported

ACCLIMATION
- Acclimation period: at least 14 days prior to the test
- Acclimation conditions (same as test or not): same as test conditions; water temperatures in the cultures ranged from 20.9 to 23.3 degrees Celcius, the pH of the water ranged from 8.3 to 8.6, dissolved oxygen concentrations were ≥8.3 mg/L (≥95% of saturation).
- Type and amount of food during acclimation: brine shrimp nauplii (Artemia sp.).
- Feeding frequency during acclimation: The fish were not fed for two days prior to the test or during the test.
- Health during acclimation (any mortality observed): During this 2-week period, the fish in the lot used for the test showed no signs of disease or stress and there were no mortalities.


FEEDING DURING TEST :
- Frequency: The fish were not fed for two days prior to the test or during the test.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Post exposure observation period:

Observations were made at 24, 48, 72 and 96 hours.

Hardness:

141 mg/L as CaCO3

Test temperature:

22 ± 1 degrees Celcius

pH:

8.1

Dissolved oxygen:

≥8.3 mg/L (≥95% of saturation)

Salinity:

Not applicable

Conductivity:

Specific Conductance: 344 µS/cm

Nominal and measured concentrations:

A single WAF loading rate of 4.0 mg active ingredient/L.

Details on test conditions:

TEST SYSTEM
- Test vessel: 25-L stainless steel aquaria
- Material, size, headspace, fill volume: The depth of the test water in a representative chamber was 11.9 cm.
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1 test chamber per concentration
- No. of vessels per control (replicates): 1 test chamber per concentration
- Biomass loading rate: 0.1572 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Freshwater obtained from a well approximately 40 meters deep located on the EAG Laboratories-Easton site.
- Total organic carbon: <2 mg C/L
- Particulate matter: The well water was passed through a sand filter to remove particles greater than approximately 25 microns, and pumped into a 37,800-L storage tank where the water was aerated with spray nozzles. Prior to use, the water was filtered to 0.45 microns to remove fine particles
- Pesticides: none, UV-sterilized
- Alkalinity: 179 mg/L as CaCO3

Key result

Duration:

96 h

Dose descriptor:

LL50

Effect conc.:

> 4 other: a.i./L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: no mortality

Key result

Duration:

96 h

Dose descriptor:

other: NOEL

Effect conc.:

4 other: a.i./L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: No mortality

Key result

Duration:

96 h

Dose descriptor:

other: Maximum WAF Loading Rate Causing 0% Mortality

Effect conc.:

4 other: a.i./L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: No mortality

Key result

Duration:

96 h

Dose descriptor:

other: Minimum WAF Loading Rate Causing 100% Mortality

Effect conc.:

> 4 other: a.i./L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: No mortality

Details on results:

Not reported

Results with reference substance (positive control):

Not applicable

Validity criteria fulfilled:

yes

Conclusions:

A 96-Hour Static Acute Toxicity Test with the Fathead Minnow (Pimephales promelas) exposed to WAF loading rate of 4.0 mg a.i./L., performed according to OECD guideline 203; U.S. EPA method 850.1075, and ASTM Standard E729-96. Based on nominal water accommodated fraction (WAF) loading rate: 96-Hour LL50: >4.0 mg a.i./L No-Observed-Effect Level: 4.0 mg a.i./L Maximum WAF Loading Rate Causing 0% Mortality 4.0 mg a.i./L Minimum WAF Loading Rate Causing 100% Mortality >4.0 mg a.i./L.

Executive summary:

A 96-Hour Static Acute Toxicity Test with the Fathead Minnow (Pimephales promelas) exposed to WAF loading rate of 4.0 mg a.i./L., performed according to OECD guideline 203; U.S. EPA method 850.1075, and ASTM Standard E729-96. Based on nominal water accommodated fraction (WAF) loading rate: 96-Hour LL50: >4.0 mg a.i./L No-Observed-Effect Level: 4.0 mg a.i./L Maximum WAF Loading Rate Causing 0% Mortality 4.0 mg a.i./L Minimum WAF Loading Rate Causing 100% Mortality >4.0 mg a.i./L.

Description of key information

A 96-Hour Static Acute Toxicity Test with the Fathead Minnow (Pimephales promelas) exposed to WAF loading rate of 4.0 mg a.i./L., performed according to OECD guideline 203; U.S. EPA method 850.1075, and ASTM Standard E729-96. Based on nominal water accommodated fraction (WAF) loading rate: 96-Hour LL50: >4.0 mg a.i./L No-Observed-Effect Level: 4.0 mg a.i./L Maximum WAF Loading Rate Causing 0% Mortality 4.0 mg a.i./L Minimum WAF Loading Rate Causing 100% Mortality >4.0 mg a.i./L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

4 mg/L