Reaction product of 4-aminophenol with 2-ethyl-6-methylbenzenamine and sodium polysulfide

Water solubility

Link to relevant study record(s)

Reference

Endpoint:

water solubility

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2017-06-06 to 2017-08-09

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 105 (Water Solubility)

Version / remarks:

July 27, 1995

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method A.6 (Water Solubility)

Version / remarks:

2008

Deviations:

no

Qualifier:

according to guideline

Guideline:

EPA OPPTS 830.7840 (Water Solubility)

Version / remarks:

March 1998

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Type of method:

flask method

Remarks:

shake flask method

Key result

Water solubility:

>= 0.53 - <= 0.61 mg/L

Conc. based on:

act. ingr.

Incubation duration:

>= 24 - <= 72 h

Temp.:

20 °C

pH:

>= 2.54 - <= 2.57

Remarks on result:

other: the water solubility of Blue Sema was determined to be 0.57 +/- 0.04 mg/L (95% confidence interval) .

Remarks:

The measured concentrations are constant within ± 15 % and thus within the range required by the guidelines.

Details on results:

The measured concentrations were constant within ± 15 % and thus within the range required by the guidelines.

Table 1: Results of the Method Validation

Measurement repeatability (7 parallels)	CV% < 1 %
Repeatability of the Preparation (n=5)	< 3 %
Linear range	0.1 - 20 µg/mL
Correlation coefficients	> 0.999
Limit of Quantification	0.1 µg/mL
Limit of Detection	0.1 µg/mL
Recovery from ISO medium	100 %
Recovery from20X AAPmedium	99 %
Recovery from ultra-pure water	110 %
Accuracy(samples used for calibration)	≤ 5 %
Precision	≤ 7 % (10 % at LOQ)
Stability of the test item inISO mediumat the temperature 18-22°C for 2 days	28 % at the test conditions
Stability of the test item in20X AAPmediumat the temperature 22-26°C for 7 days	58 % at the test conditions
Stock solution stability (1 mg/ml), after dissolution, dye complexation and color development	for at least 5 days at 5±3 °C

Table 3: Regression data

Analytical occasion	Constant	X Coefficient	R. Squared
June 20, 2017	0.0028	0.0943	0.9994

Table 3: Results of the determination of Blue Sema concentrations

Sampling time	Measured concentrationofBlue Sema(mg/L )		Measured pH
	Test vessels	Mean withthe 95% confidence intervals
After 24h	0.57	0.55 ± 0.09	2.57
	0.56		2.55
	0.51		2.56
After 48h	0.53	0.54 ± 0.09	2.54
	0.58		2.55
	0.51		2.55

Table 4: Results of the determination of Blue Sema concentrations (continued)

Sampling time	Measured concentrationofBlue Sema(mg/L )				Measured pH
	Test vessels		Mean withthe 95% confidence intervals
After 72h	0.60		0.61 ± 0.13		2.54
	0.67				2.54
	0.56				2.54
Solubility ofthe test item (mg/L)	0.57 ± 0.04	-
RSD%	9	-
Ultra-pure water			< LOQ	5.83

Conclusions:

The water solubility of the test item was determined to be 0.57 ± 0.04 mg/L (95% confidence interval) at 20 °C ± 1 °C using the shake-flask method for the performance.

Executive summary:

The water solubility of the test item was dertermined at 20 ± 1 °C according to OECD TG 105 using the shake-flask method. The water solubility: 0.57 ± 0.04 mg/L (95% confidence interval).

 

Description of key information

The water solubility of Blue Sema was determined to be 0.57 ± 0.04 mg/L (95% confidence interval)  at 20 °C ± 1 °C using the shake-flask method for the performance 

Key value for chemical safety assessment

Water solubility:

0.57 mg/L

at the temperature of:

20 °C

Additional information

The water solubility of the test item was dertermined at 20 ± 1 °C according to OECD TG 105 using the shake-flask method. The water solubility is 0.57 ± 0.04 mg/L (95% confidence interval).