1,6-dichloro-1,6-dideoxy-β-D-fructofuranosyl 4-chloro-4-deoxy-α-D-galactose

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1983-06-03 to 1984-04-27

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Version / remarks:

version adopted 12 May 1981

GLP compliance:

yes

Specific details on test material used for the study:

SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 153006
- Purity test date: 97.7%

STABILITY AND STORAGE CONDITIONS OF TEST MATERIAL
- Storage condition of test material:
-- sample 1: in the dark at ambient temperature
-- sample 2: wrapped in aluminium at 4°C

Analytical monitoring:

yes

Details on sampling:

- Concentrations: all concentrations in the definitive test (nominal: 0, 180, 560, 1000 and 1800 mg/L)
- Sampling method: samples at the start of the test were taken from preparation flasks, samples at the end of the test were taken from pooled solution for each concentration and the control; samples were analysed by the sponsor
- Sample storage conditions before analysis: frozen

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: a stock solution of 10000 mg/L was prepared for dilution using borehole water
- Evidence of undissolved material: minute black particulate matter was observed in the stock solution prior to the start of the preliminary test

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Source: Shell Toxicology Laboratory, Sittingbourne
- Feeding during test: non

ACCLIMATION
- Type and amount of food: unicellular green alga (Chlorelle) and yeast ("Allinsons" dried baking yeast)
- Feeding frequency: daily

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
Gravid female Daphnia were removed from culture before the start of the test and placed in borehole water at approx. 20°C. Juveniles were removd each day. On the day of the test less than 24h old juvenile Daphnia were separated from the females by careful pipetting.

Test type:

static

Water media type:

freshwater

Remarks:

chalk borehole water

Limit test:

no

Total exposure duration:

48 h

Hardness:

start of the test: 244 - 266 mg/L as CaCO3
end of the test: 190 - 206 mg/L as CaCO3

Test temperature:

start of the test: 20.1 - 21.0°C
end of the test: 20.0 - 20.9°C

pH:

start of the test: 7.7 - 7.8
end of the test: 7.9 - 8.0

Dissolved oxygen:

start of the test: 98 - 100%
end of the test: 81 - 90%

Nominal and measured concentrations:

nominal: 0, 180, 560, 1000 and 1800 mg/L
measured: not detectable, not given, 538 - 562 mg/L, not given, 1716 - 1789 mg/L

Details on test conditions:

TEST SYSTEM
- Material, size, headspace, fill volume: 120 mL Pyrex glass crystallising dishes covered by a loose fitting watch glass, previously washed in 2% "Decon 90" and rinsed with borehole water
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: chalk borehole water
- Culture medium different from test medium: no

OTHER TEST CONDITIONS
- Photoperiod: 14 h light:10 h dark

RANGE-FINDING STUDY
- Test concentrations: control, 180, 560 and 1000 mg/L, with each two replicates containing each 5 Daphnia

Reference substance (positive control):

not specified

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1 800 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

exposure concentration [mg/L]	No. Daphnia exposed	No. Daphnia mobile		No. Daphnia immobile
		24h	48h	24h	48h
control	40	40	39	0	1
180	40	40	38	0	2
560	40	40	40	0	0
1000	40	40	38	0	2
1800	40	40	40	0	0

Validity criteria fulfilled:

yes

Conclusions:

The toxicity of the test item on Daphnia magna was determined in a GLP study according to OECD 202. The 48-h EC50 was determined to be >1800 mg/L (nominal).

Description of key information

The toxicity of the test item on Daphnia magna was determined in a GLP study according to OECD 202. The 48-h EC50 was determined to be >1800 mg/L (nominal).

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1 800 mg/L

Additional information

The toxicity of test item on Daphnia magna was determined in a GLP study according to OECD 202. Individuals of D. magna were exposed to 0 (control), 180, 560, 1000 and 1800 mg/L of the test substance in chalk borehole water, equivalent to geometric mean measured concentrations of <LOQ, not analysed, 546, not analysed and 1738 mg/L after 48 h. No immobility or mortality of D. magna was observed after 48 h at 1800 mg/L. The 48 h EC50 value of >1800 mg/L (nominal) was concluded.