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EC number: 613-953-8 | CAS number: 66603-10-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Begin of the study: 27 Feb 2002; experimental phase: 05 Mar 2002 - 07 Mar 2002
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Version / remarks:
- 4 April 1984
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Cyclohexylhydroxydiazene 1-oxide, potassium salt
- EC Number:
- 613-953-8
- Cas Number:
- 66603-10-9
- Molecular formula:
- C6H11KN2O2
- IUPAC Name:
- Cyclohexylhydroxydiazene 1-oxide, potassium salt
- Test material form:
- liquid
- Details on test material:
- Xyligen 30F is a 30% aqueous solution of K-HDO.
Constituent 1
- Specific details on test material used for the study:
- see table below
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- The analyses were carried out as a separate study at the test facility Analytical Department of BASF Aktiengesellschaft under the responsibility of the Study Director of this test facility. The study was carried out in compliance with the Principles of Good Laboratory Practice. The concentration control analyses were performed with test solutions of the following nominal concentrations (mg/L) of the test substance: 0 – 100 – 6.25
Test solutions
- Vehicle:
- yes
- Remarks:
- "M4 water"
- Details on test solutions:
- PREPARATION OF THE STOCK SOLUTION AND DILUTION:
The test substance (Xyligen 30 F) was stirred in M4 medium for about 10 minutes at 20 ± 2°C. The nominal concentration of the stock solution was 100 mg/L.
By diluting this stock solution with M4 medium the following nominal concentrations (mg/L) were prepared: 0 (control) - 100 – 50 – 25 – 12.5 – 6.25
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM:
- Common name: Daphnia magna Strauss
- Strain/clone: The clone of Daphnia magna STRAUS 1820 used was supplied by the Institut National de Recherche Chimique Appliquée, France, in 1978. From this date on this clone was cultured and bred continuously in the Laboratory Experimental Toxicology and Ecology of BASF AG in Ludwigshafen.
- Source: Institut National de Recherche Chimique Appliquée
- Age of parental stock (mean and range, SD): 2-4 weeks
- Age of the animals at the begin of exposition: 2 - 24 h (starting with the 3rd breed of parent animals)
- Feeding during test: no
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Not required for this kind of test.
Test conditions
- Hardness:
- Total hardness: 2.20 – 3.20 mmol/L
- Test temperature:
- 18 - 22 °C (max. temperature difference 2 °C)
- pH:
- pH value: 7.5 -8.5
- Dissolved oxygen:
- Test medium was saturated with oxygen
- Salinity:
- Molar ratio Ca : Mg: about 4 : 1
- Conductivity:
- Conductivity: 550 - 650 µS/cm
- Nominal and measured concentrations:
- Measured concentrations correspond to the nominal concentrations (recovery rate ≥ 98 %). Details are presented in the table below.
- Details on test conditions:
- TEST SYSTEM
see table below
RANGE-FINDING STUDY
- Test concentrations: The test concentrations were determined in a range finding test. - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate
Results and discussion
Effect concentrationsopen allclose all
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC0
- Effect conc.:
- >= 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC100
- Effect conc.:
- > 100 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- See table below
- Results with reference substance (positive control):
- The EC50(24h) of the control substance potassium dichromate (non-GLP) was 1.07 mg/L (Date of the last control experiment : 18 Feb 2002, project number: 97/0242/50/72).
- Reported statistics and error estimates:
- Due to the results of the test no statistical evaluation of the data was performed.
Any other information on results incl. tables
Number of mobile Daphnids in the different test concentrations:
Conc. |
Parallel 1 |
Parallel 2 |
Parallel 3 |
Parallel 4 |
∑ |
∑ |
||||||||
[mg/L] |
[mg/L] |
[mg/L] |
[mg/L] |
[mg/L] |
h |
|||||||||
0 |
24 |
48 |
0 |
24 |
48 |
0 |
24 |
48 |
0 |
24 |
48 |
24 |
48 |
|
Control |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
20 |
20 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
100 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
4 |
5 |
4 |
4 |
19 |
18 |
50 |
5 |
5 |
5 |
5 |
4 |
4 |
5 |
5 |
5 |
5 |
5 |
5 |
19 |
19 |
25 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
20 |
20 |
12.5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
20 |
20 |
6.25 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
5 |
20 |
20 |
Number of test animals per replicate: 5
Number of test animals per concentration: 20
Concentration control analysis:
Analytically measured relative minimum and maximum concentrations of the test substance in the test solutions.
Time [h] |
Stocked with daphnids |
Minimum [%] |
Maximum [%] |
0 |
No |
100.5 |
102.0 |
48 |
No |
98.0 |
100.0 |
The values are given in percent of the nominal concentrations:
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- EC50 (48 h) and EC100 (48 h) > 100 mg/L Xyligen 30 F (K-HDO 30 % in water) .
VALIDITY CRITERIA:
• In the control the immobilization was ≤ 10%.
• In the control no daphnids were captured in a surface film.
• The O2-concentration was > 3 mg/L in all tested replicates.
• The EC50(24h) of the control substance potassium dichromate (non-GLP) was 1.07 mg/L
(Date of the last control experiment: 18 Feb 2002, project number: 97/0242/50/72).
The test is valid. - Executive summary:
The test substance was tested in the range of concentrations between 100 mg/L and 6.25 mg/L. The dilution factor was 2. Instead of mortality the test criterion of acute toxicity is immobilization of the daphnids after 48 hours of exposure which is expressed as EC (effect concentration). Those animals were considered to be immobile which were unable to swim within 15 seconds even after gentle agitation of the test vessel.
According to the results of the test the EC50 and EC100 value is greater than 100 mg/L.
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