2-Naphthalenesulfonic acid, 7-amino-4-hydroxy-3-[2-[4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-8-[2-[2-sulfo-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-, sodium salt (1:4)

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Remarks:

Experimental data of read across chemical

Justification for type of information:

Data for the target chemical is summarized based on the structurally similar read across chemicals.

Reason / purpose for cross-reference:

read-across source

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: As mention in below principle

Principles of method if other than guideline:

WoE report is prepared based on toxicity to fish study:1st, 2nd study

GLP compliance:

not specified

Specific details on test material used for the study:

- Name of test material: 2-Naphthalenesulfonic acid, 7-amino-4-hydroxy-3-[2-[4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-8-[2-[2-sulfo-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-, sodium salt (1:4)- Molecular formula: C26H25N5O19S6.4Na- Molecular weight: 995.85 g/mole- Smiles : [Na][Na][Na][Na]Nc1ccc2c(cc(S(O)(=O)=O)c(N=Nc3ccc(S(=O)(=O)CCOS(O)(=O)=O)cc3)c2O)c1N=Nc1ccc(S(=O)(=O)CCOS(O)(=O)=O) cc1S(O)(=O)=O- Substance type: Organic- Physical state: Solid powder

Analytical monitoring:

not specified

Vehicle:

not specified

Details on test solutions:

1. The test substance was poorly soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with 48 hr stirring for achieving test concentrations of 100mg/L ,respectively.2. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with 2 hrs continuous stirring for achieving test concentrations of 100mg/L,respectively.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

1. TEST ORGANISM- Common name:ZEBRA FISH - Source:UNIQUE AQUARIUM, NAGPUR- Length at study initiation (length definition, mean, range and SD): average 1.58cm- Weight at study initiation (mean and range, SD): 0.0836 g (average)ACCLIMATION- Acclimation period: 7 days- Type and amount of food during acclimation: Standard brand feed- Feeding frequency during acclimation: Daily once- Health during acclimation (any mortality observed):During last 48 hours = noFEEDING DURING TEST- Food type: Standard brand feed- Frequency: Withdrawal of food 1 day before and during exposure

Test type:

static

Water media type:

freshwater

Total exposure duration:

96 h

Post exposure observation period:

24, 48, 72 and 96hrs

Hardness:

1. 138.6 (mg of CaCO3)2. 148.5 (mg of CaCO3)

Test temperature:

1. 23 °C

pH:

1. 7.25- 7.62 2. 7.04

Dissolved oxygen:

1. 8.0 mg/l2. 7.8 (mg/l)

Nominal and measured concentrations:

Nominal 100 mg/l

Details on test conditions:

TEST SYSTEM - Test vessel: Plastic aquaria - Type (delete if not applicable): open / closed: No data - Material, size, headspace, fill volume: The test vessels used were all plastic aquaria having the water capacity of 5liters filled with 2 liter of water. - Aeration: Aeration in test vessels was provided 1 day before the start of experiment - No. of organisms per vessel: 8 - No. of vessels per concentration (replicates): 2 - No. of vessels per control (replicates): 2 - No. of vessels per vehicle control (replicates): 2 OTHER TEST CONDITIONS - Adjustment of pH: yes - Photoperiod: 16 hours light and 8 hours dark EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Symptoms, pH, tempterature and Dissolved oxygen

Reference substance (positive control):

not specified

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: 1st study and 2nd study

Sublethal observations / clinical signs:

1. Mortality and symptoms:

Nominal Concentration (mg/l)	Number of fish		Mortality (cumulated) after
			3 h		6 h		24h		48h		72h		96h
			V1	V2	V1	V2	V1	V2	V1	V2	V1	V2	V1	V2
Control	4	4	-	-	-	-	-	-	-	-	-	-	-	-
100	4	4	-	-	-	-	-	-	-	-	-	-	-	-

 

Visible Symptoms:

Nominal Concentration (mg/l)

Number of fish

symptom (cumulated) after

3 h

6 h

24h

48h

72h

96h

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

V1

V2

Control

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

100

4

4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

NS4

Explanation of symptoms:

A: apathy

N: Narcotic-like state

C: Swimming near water surface

M: Slow movements compared to control

NS: No abnormal symptoms

S: Surfacing behaviour

L: Loss of equilibrium

SM: Shimming movement

H1: Hyperactivity

H2: Hypo activity

B: Bending behavior

F: Fin rot

IS: Invert Swimming

SB: Swimming behaviour

RF: Respiratory Function

P: Pigmentation

Validity criteria fulfilled:

not specified

Conclusions:

1. Based on mortality of fishes due to the exposure of test chemical at nominal concentration 100 mg/l, experimental median lethal Concentrations [LC-50 (96 h)] on Zebra fish(Danio rerio) was determine to be > 100 mg/l.2. Based on mortality of fishes due to the exposure of test chemical at nominal concentration 100 mg/l, experimental median lethal Concentrations [LC-50 (96 h)] on Zebra fish (Danio rerio) was determine to be > 100 mg/l.Thus based on the above studies, 2-Naphthalenesulfonic acid, 7-amino-4-hydroxy-3-[2-[4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-8-[2-[2-sulfo-4-[[2-(sulfooxy)ethyl]sulfonyl] phenyl]diazenyl]-, sodium salt (1:4) (CAS no 607724 -37 -8) consider to be nontoxic to fish and not classified as per the CLP classification criteria.

Executive summary:

Various studies available for the test chemical were reviewed to determine the toxic nature of 2-Naphthalenesulfonic acid, 7-amino-4-hydroxy-3-[2-[4-[[2-(sulfooxy)ethyl]sulfonyl] phenyl]diazenyl]-8-[2-[2-sulfo-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-, sodium salt (1:4) (CAS no 607724 -37 -8) on the mortality of fish. The studies are as mentioned below:

In the first study Fish Acute Toxicity test was performed to evaluate the toxic nature of test chemical. Test was performed according to OECD Guideline 203. The test substance was poorly soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with 48 hr stirring for achieving test concentrations of 100mg/L ,respectively. The test was performed on the limit concentration i.e nominal concentration selected for the experiment were 100 mg/L and test fish were exposed to these concentration for 96 hours. After 96hrs of exposure LC0, LC50 and LC100 was observed. Effect on the symptoms and the normal activity was checked in the interval of 24rs. pH, tempterature and dissolved oxygen was also checked.

The lethal concentrations LC50 was determine to be >100 mg/L. LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L. LC50 (96 hours) Experimental = >100 mg/L. Thus based on the LC50 it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Similarly Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with 2 hrs continuous stirring for achieving test concentrations of 100mg/L, respectively. The test was performed on the limit concentration i.e nominal concentration selected for the experiment were 100 mg/L and test fish were exposed to these concentration for 96 hours. After 96hrs of exposure LC0, LC50 and LC100 was observed. Effect on the symptoms and the normal activity was checked in the interval of 24rs. pH, tempterature and dissolved oxygen was also checked. The lethal concentrations LC50 was determine to be >100 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L

LC50 (96 hours) Experimental = >100 mg/L

Thus based on the LC50 it can be concluded that the chemical was nontoxic to fish and can be consider to be not classified as per the CLP classification criteria.

Thus based on the above studies, 2-Naphthalenesulfonic acid, 7-amino-4-hydroxy-3-[2-[4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-8-[2-[2-sulfo-4-[[2-(sulfooxy)ethyl]sulfonyl] phenyl]diazenyl]-, sodium salt (1:4) (CAS no 607724 -37 -8) consider to be nontoxic to fish and not classified as per the CLP classification criteria.

Description of key information

1. Based on mortality of fishes due to the exposure of test chemical at nominal concentration 100 mg/l, experimental median lethal Concentrations [LC-50 (96 h)] on Zebra fish(Danio rerio) was determine to be > 100 mg/l.

2. Based on mortality of fishes due to the exposure of test chemical at nominal concentration 100 mg/l, experimental median lethal Concentrations [LC-50 (96 h)] on Zebra fish (Danio rerio) was determine to be > 100 mg/l.

Thus based on the above studies, 2-Naphthalenesulfonic acid, 7-amino-4-hydroxy-3-[2-[4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-8-[2-[2-sulfo-4-[[2-(sulfooxy)ethyl]sulfonyl] phenyl]diazenyl]-, sodium salt (1:4) (CAS no 607724 -37 -8) consider to be nontoxic to fish and not classified as per the CLP classification criteria.

Additional information

Various studies available for the test chemical were reviewed to determine the toxic nature of 2-Naphthalenesulfonic acid, 7-amino-4-hydroxy-3-[2-[4-[[2-(sulfooxy)ethyl]sulfonyl] phenyl]diazenyl]-8-[2-[2-sulfo-4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-, sodium salt (1:4) (CAS no 607724 -37 -8) on the mortality of fish. The studies are as mentioned below:

In the first study Fish Acute Toxicity test was performed to evaluate the toxic nature of test chemical. Test was performed according to OECD Guideline 203. The test substance was poorly soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with 48 hr stirring for achieving test concentrations of 100mg/L ,respectively. The test was performed on the limit concentration i.e nominal concentration selected for the experiment were 100 mg/L and test fish were exposed to these concentration for 96 hours. After 96hrs of exposure LC0, LC50 and LC100 was observed. Effect on the symptoms and the normal activity was checked in the interval of 24rs. pH, tempterature and dissolved oxygen was also checked.

The lethal concentrations LC50 was determine to be >100 mg/L. LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L. LC50 (96 hours) Experimental = >100 mg/L. Thus based on the LC50 it can be concluded that the chemical was nontoxic and can be consider to be not classified as per the CLP classification criteria.

Similarly Fish Acute Toxicity test according to OECD Guideline 203 was conducted for test chemical. The test substance was soluble in water. Therefore, the test solution was prepared by dissolving 400 mg of the test substance in 4 liters of potable water (passed through reverse osmosis system) with 2 hrs continuous stirring for achieving test concentrations of 100mg/L, respectively. The test was performed on the limit concentration i.e nominal concentration selected for the experiment were 100 mg/L and test fish were exposed to these concentration for 96 hours. After 96hrs of exposure LC0, LC50 and LC100 was observed. Effect on the symptoms and the normal activity was checked in the interval of 24rs. pH, tempterature and dissolved oxygen was also checked. The lethal concentrations LC50 was determine to be >100 mg/L

LC0 (96 hours) (highest loading at which no mortality was observed) = 100 mg/L

LC50 (96 hours) Experimental = >100 mg/L

Thus based on the LC50 it can be concluded that the chemical was nontoxic to fish and can be consider to be not classified as per the CLP classification criteria.

Thus based on the above studies, 2-Naphthalenesulfonic acid, 7-amino-4-hydroxy-3-[2-[4-[[2-(sulfooxy)ethyl]sulfonyl]phenyl]diazenyl]-8-[2-[2-sulfo-4-[[2-(sulfooxy)ethyl]sulfonyl] phenyl]diazenyl]-, sodium salt (1:4) (CAS no 607724 -37 -8) consider to be nontoxic to fish and not classified as per the CLP classification criteria.