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Diss Factsheets
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EC number: 945-069-6 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 20 - 22 Jan 2004
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study with acceptable restrictions: The analytical purity is only 77.2%.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- GLP compliance:
- yes
- Remarks:
- according to the Japanese GLP Standard
- Analytical monitoring:
- yes
- Details on sampling:
- - Sampling method: Samples were taken directly from each vessel.
- Sample storage conditions before analysis: no storage before analysis - Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test substance was measured and mixed with vehicle. Water was added bit by bit to prepare the stock solution (1000 mg/L). Stock solution was diluted with test water and stored in the dark and cool. The stability during 48 hours is confirmed.
- Controls: blank control and vehicle control
- Chemical name of vehicle: HCO-40
- Concentration of vehicle in test medium: 1000 mg/L in a stock solution, 1 mg/L in final concentration
- Evidence of undissolved material: Test water was clear and colourless after preparation. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: water flea
- Source: Incorporated Administrative Agency, National Institute for Environmental Studies, Tsukuba, Japan
- Age at study initiation: ≤ 24 hours
- Feeding during test: no food
ACCLIMATION
- Acclimation period for parental animals: 13 Jan - 04 Feb 2004
- Acclimation conditions: same as test
- Type and amount of food: Chlorella vulgaris, 0.2 mg C/day/animal
- Health during acclimation: Only healthy stock was used (mortality of parental animal was < 0%). - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Hardness:
- 248 - 250 mg/L as CaCO3
- Test temperature:
- 19.6 - 19.9 °C
- pH:
- 8.3 - 8.4
- Dissolved oxygen:
- 8.6 - 8.7 mg/L
- Nominal and measured concentrations:
- Nominal concentration: 0.1 mg/L
Measured concentration: 0.0931 mg/L (geometric mean) - Details on test conditions:
- TEST SYSTEM
- Test vessel: beaker
- Type: closed with a teflon sheet
- Material: glass; Size: 100 mL; Fill volume: 100 mL
- Renewal rate of test solution: every 24 hours
- No. of organisms per vessel: 5
- No. of vessels per concentration: 4
- No. of vessels per control: 4
- No. of vessels per vehicle control: 4
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Elendt M4
- Culture medium different from test medium: no
- Intervals of water quality measurement: Temperature, water hardness, pH and oxygen were measured at the study inition and after 24 h exposure time.
OTHER TEST CONDITIONS
- Adjustment of pH: no adjustment
- Photoperiod: 16 hours in the light, 8 hours in the dark
- Light intensity: < 800 lux
EFFECT PARAMETERS MEASURED: General condition and immobility were observed after 24 hours and 48 hours exposure time.
TEST CONCENTRATIONS
- Justification for using less concentrations than requested by guideline: 0.1 mg/L is the maximum attainable concentration under the present test conditions and preparation methods.
- Range finding study: performed
- Test concentrations: 0.0006, 0.006, and 0.06 mg/L
- Results used to determine the conditions for the definitive study: Immobilisation was not observed in all groups. - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (analytical grade)
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- > 0.093 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Details on results:
- - Behavioural abnormalities: No abnormality was found.
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 0.76 ± 0.16 mg/L
Reference
Table 1: Measured concentration
Nominal Concentration [mg/L] |
Measured Concentration [mg/L]; (Percent of nominal) |
||
0 h (new)
|
24 h (old) |
Mean |
|
Control |
<0.0002 |
<0.0002 |
- |
Solvent Control |
<0.0002 |
<0.0002 |
- |
0.1 |
0.0922 (92) |
0.0940 (94) |
0.0931 |
new: freshly prepared test solutions
old: test solutuions after 24 hours
Table 2: The number of immobilized Daphnia magna (percent immobility)
Nominal Concentration [mg/L] |
Geometric measured Concentration [mg/L] |
Cumulative Number of Immobilized Daphnia (Percent Immobility) |
|
24 h |
48 h |
||
Control |
- |
1 (5) |
1 (5) |
Solvent Control |
- |
0 (0) |
0 (0) |
0.1 |
0.0931 |
0 (0) |
0 (0) |
Description of key information
In a 48 hours test daphnia were exposed to bis(2-ethylhexyl) azelate at a nominal concentration of 0.1 mg/L (measured 0.093 mg/L). At this concentration, which is above the maximum water solubility of bis(2-ethylhexyl) azelate no effects on mobility were observed. Therefore it can be concluded that the EC50 is > 0.1 mg/L (MOE 2004).
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 0.1 mg/L
Additional information
The substance tested is considered a close analogue of diesters of alcohols, C7-9-iso-, C8-rich, 2-ethylhexyl and nonanedioic acid and a worst case due to the presence of 2-ethylhexan-1-ol as part of the starting materials of both diesters of alcohols, C7-9-iso-, C8-rich, 2-ethylhexyl and nonanedioic acid and bis(2-ethylhexyl) azelate. Therefore the outcome of the study that indicates no toxicity at the water solubility level is considered representative for diesters of alcohols, C7-9-iso-, C8-rich, 2-ethylhexyl and nonanedioic acid (see rationale for read-across)
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